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NASP (NM_152298) Human Tagged ORF Clone
CAT＃: RG208783
- TrueORF®
NASP (tGFP-tagged) - Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3
ORF Plasmid: DDK
"NM_152298" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FLB7527; HMDRA1; PRO1999 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208783 representing NM_152298
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAAATTGAAGATG TTCCTGCTCCTTCTACATCTGCAGATAAAGTGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTATT GGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCAGCAGCTGTCAATGCATTCCAGGAAGCAGCT AGTCTTTTAGGTAAGAAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAAT CACTTCTGGAGTTGGCAAGAATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGA GGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGAAACTGAAGGC TCAGAAGAGGATGATAAAGAAAATGATAAGACCGAAGAAATGCCAAATGATTCAGTCCTTGAAAACAAGT CTCTTCAAGAAAATGAGGAGGAGGAGATTGGGAACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAA GATCATTTTTAAAAGGCAAGAAACAAAAGAAGCACAGCTTTATGCTGCCCAGGCACATCTTAAACTCGGA GAAGTTAGTGTTGAATCTGAAAACTATGTGCAAGCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTGCAGG AACAGTACCTGGAAGCCCACGACCGTCTCCTTGCAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTA CAACTCTCAGTATGATGAGGCAGTGGCACAGTTCAGCAAATCTATTGAAGTCATTGAGAACAGAATGGCT GTACTAAACGAGCAGGTGAAGGAGGCTGAAGGATCGTCTGCTGAATACAAGAAAGAAATTGAGGAACTAA AGGAACTGCTACCCGAAATTAGAGAGAAGATAGAAGATGCAAAGGAGTCTCAGCGTAGTGGGAATGTAGC TGAACTGGCTCTGAAAGCTACTCTGGTGGAGAGTTCTACTTCAGGTTTCACTCCTGGTGGAGGAGGCTCT TCAGTCTCCATGATTGCCAGTAGAAAGCCAACAGACGGTGCTTCCTCATCAAATTGTGTGACTGATATTT CCCACCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCCAA ACAAGAGCCGGAGGTGAACGGAGGCAGTGGGGATGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATG GAGGAGGAGGCTGAGAATCAGGCTGAAAGCCGGGCAGCAGTGGAGGGGACAGTGGAGGCTGGAGCTACAG TTGAAAGCACTGCATGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208783 representing NM_152298
Red=Cloning site Green=Tags(s) MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDETEG SEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLG EVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMA VLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGS SVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENM EEEAENQAESRAAVEGTVEAGATVESTAC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152298 |
| ORF Size | 1347 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152298.4 |
| RefSeq Size | 2284 bp |
| RefSeq ORF | 1350 bp |
| Locus ID | 4678 |
| UniProt ID | P49321 |
| Domains | TPR |
| Gene Summary | This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208783 | NASP (Myc-DDK-tagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3 |
CNY 3,990.00 |
|
| RC208783L3 | Lenti-ORF clone of NASP (Myc-DDK-tagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3 |
CNY 5,890.00 |
|
| RC208783L4 | Lenti-ORF clone of NASP (mGFP-tagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3 |
CNY 5,890.00 |
|
| SC109477 | NASP (untagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3 |
CNY 2,950.00 |
