CFAP298 (NM_021254) Human Tagged ORF Clone

CAT#: RG200169

  • TrueORF®

C21orf59 (tGFP-tagged) - Human chromosome 21 open reading frame 59 (C21orf59), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_021254" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


C21orf59 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms C21orf48; C21orf59; CILD26; FBB18; Kur
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG200169 representing NM_021254
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTGCTGCACGTGAAGCGGGGCGACGAGAGCCAGTTCCTGCTGCAGGCGCCTGGGAGTACCGAGC
TGGAGGAGCTCACGGTGCAGGTGGCCCGGGTCTATAATGGGCGGCTCAAGGTGCAGCGCCTCTGCTCAGA
AATGGAAGAATTAGCCGAACATGGCATATTTCTCCCTCCTAATATGCAAGGACTGACCGATGATCAGATT
GAAGAATTGAAATTGAAGGATGAATGGGGTGAAAAATGCGTACCCAGCGGAGGTGCAGTGTTTAAAAAGG
ATGATATTGGACGAAGGAATGGGCAAGCTCCAAATGAGAAGATGAAGCAAGTGTTAAAGAAGACTATAGA
AGAAGCCAAAGCAATAATATCTAAGAAACAAGTGGAAGCCGGTGTCTGTGTTACCATGGAGATGGTGAAA
GATGCCTTGGACCAGCTTCGAGGCGCGGTGATGATTGTTTACCCCATGGGGTTGCCACCGTATGATCCCA
TCCGCATGGAGTTTGAAAATAAGGAAGACTTGTCGGGAACACAGGCAGGGCTCAACGTCATTAAAGAGGC
AGAGGCGCAGCTGTGGTGGGCAGCCAAGGAGCTGAGAAGAACGAAGAAGCTTTCAGACTACGTGGGGAAG
AATGAAAAAACCAAAATTATCGCCAAGATTCAGCAAAGGGGACAGGGAGCTCCAGCCCGAGAGCCTATTA
TTAGCAGTGAGGAGCAGAAGCAGCTGATGCTGTACTATCACAGAAGACAAGAGGAGCTCAAGAGATTGGA
AGAAAATGATGATGATGCCTATTTAAACTCACCATGGGCGGATAACACTGCTTTGAAAAGACATTTTCAT
GGAGTGAAAGACATAAAGTGGAGACCAAGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG200169 representing NM_021254
Red=Cloning site Green=Tags(s)

MVLLHVKRGDESQFLLQAPGSTELEELTVQVARVYNGRLKVQRLCSEMEELAEHGIFLPPNMQGLTDDQI
EELKLKDEWGEKCVPSGGAVFKKDDIGRRNGQAPNEKMKQVLKKTIEEAKAIISKKQVEAGVCVTMEMVK
DALDQLRGAVMIVYPMGLPPYDPIRMEFENKEDLSGTQAGLNVIKEAEAQLWWAAKELRRTKKLSDYVGK
NEKTKIIAKIQQRGQGAPAREPIISSEEQKQLMLYYHRRQEELKRLEENDDDAYLNSPWADNTALKRHFH
GVKDIKWRPR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021254
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021254.4
RefSeq Size 1064 bp
RefSeq ORF 873 bp
Locus ID 56683
UniProt ID P57076
Gene Summary This gene encodes a protein that plays a critical role in dynein arm assembly and motile cilia function. Mutations in this gene result in primary ciliary dyskinesia. Naturally occuring readthrough transcription occurs from this locus to the downstream t-complex 10 like (TCP10L) gene. [provided by RefSeq, Apr 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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