ATP7A (NM_001282224) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DSMAX; MK; MNK; SMAX3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240136 representing NM_001282224 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTATGGGTGTGAATTCTGTTACCATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTT GGACCATTGAGCAGCAGATTGGAAAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAA TGCAACTATTATTTATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGC TTTGATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACTGTTACGG CGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAGGGTGTGACAGACATTAA AATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCTTCTATAGTGAATGCCAATCAGATAAAA GAGCTGGTTCCAGAACTCAGTTTAGATACTGGGACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATA GTATGGCTCAAGCTGGTGAAGTCGTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG CACTATTGAAGGAAAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAA GCTACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCTATGGGCT TTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGATGTAGAACGTCTAAAGAA CACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCATCATATACCAATGATTCAACAGCCACT TTCATCATTGATGGCATGCATTGTAAATCATGTGTGTCAAATATTGAAAGTACTTTATCTGCACTCCAAT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCAC TCCAGAATCCCTGAGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAA GTTGAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTTAGCCAGC CTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGTGTGCAGTCTATTGAGGG TGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCCCTTGCAAATAGCAATGGGACTGTTGAG TATGATCCTCTACTAACCTCTCCAGAAACGTTGAGAGGAGCAATAGAAGACATGGGATTTGATGCTACCT TGTCAGACACGAATGAGCCGTTGGTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAAC TAATGAATTTTATACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGT TACATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGGCGGGAAG AAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGGTATAATCCTGCTGTTAT ACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTTGGAGCCACTGTGATAGAAAATGCTGAT GAAGGAGATGGTGTTTTGGAACTTGTTGTGAGGGGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGT CTAGTCTCACAAAACACAGAGGGATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAA ATATGACCCAGAAATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCT TTGGTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGACGGTCTT TTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATGATGGTTATGGACCACCA CTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAAATGATCAACCTTCATTCTTCTATGTTC CTGGAGCGCCAGATTCTTCCAGGATTGTCTGTTATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTAC AGGGCAAAACATCAGAGGCTCTTGCAAAGTTAATTTCACTACAAGCTACAGAAGCAACTATTGTAACTCT TGATTCTGATAATATCCTCCTCAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT AAAGTAGTTCCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTGAAGGACATTCTATGGTAGATGAGT CCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACAGTGATTGCTGGTTCCATTAA CCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGACACAACCCTTTCTCAAATTGTCAAA CTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTATTTTG TTCCTTTTATTGTTTTTGTTTCCATTGCCACCCTCTTGGTATGGATTGTAATTGGATTTCTGAATTTTGA AATTGTGGAAACCTACTTTCCTGGCTACAATAGAAGTATCTCCCGAACAGAAACGATAATACGATTTGCT TTCCAAGCCTCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCACTGGGACTGGCCACTCCAACTGCTG TGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAAAGGTGGAGAGCCATTGGAGATGGC TCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACTCACGGAACCCCAGTGGTGAATCAA GTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCATAAAATCTTGGCCATTGTGGGAACTGCTGAAA GTAACAGTGAACACCCTCTAGGAACAGCCATAACCAAATATTGCAAACAGGAGCTGGACACTGAAACCTT GGGTACCTGCATAGATTTCCAGGTTGTGCCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGC TTGCTACATAAGAATAACTGGAATATAGAGGACAATAATATTAAAAATGCATCCCTGGTTCAAATTGATG CCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGCCCAGATCTCAAATGCTCTTAATGC TCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGAAATGGTCTTGTCATTAATAACGAT GTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGGACTGCTGTATTAGTAGCAGTTGATGATGAGC TGTGTGGCTTGATAGCCATTGCAGACACAGTGAAGCCTGAAGCAGAACTGGCTATCCATATTCTGAAATC TATGGGCTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTAGATCTATTGCTTCTCAGGTT GGCATTACTAAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTGCTAAAGTGAAGCAACTTCAAGAGG AGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATGGCTAATGTGGG AATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTGGTTTTGATAAGGAATGATCTT CTGGATGTAGTGGCAAGTATTGACTTATCAAGAAAGACAGTCAAGAGGATTCGGATAAATTTTGTCTTTG CTCTAATTTATAATCTGGTTGGAATTCCCATAGCTGCTGGAGTTTTTATGCCCATTGGTTTGGTTTTGCA GCCCTGGATGGGATCTGCAGCAATGGCTGCTTCATCTGTTTCTGTAGTACTTTCTTCTCTCTTCCTTAAA CTTTACAGGAAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGAGCCAGATAGGACAGAAGAGTCCTT CAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTCTCCTAAACTGGGTTTGCTGGACCG GATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGATAAACGCTCCCTAAACAGTGTTGTT ACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGACTTCAGGGAAGATGATGACACTGCATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240136 representing NM_001282224 Red=Cloning site Green=Tags(s) MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMG FDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIK ELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQE ATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTAT FIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSE VESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVE YDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKC YIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENAD EGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEAS LVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMF LERQILPGLSVMNLLSFLLCVPVQGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDII KVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVK LVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFA FQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQ VKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEG LLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINND VNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQV GITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDL LDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLK LYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVV TSEPDKHSLLVGDFREDDDTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282224
ORF Size	4266 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282224.1, NP_001269153.1
RefSeq Size	8306 bp
RefSeq ORF	4269 bp
Locus ID	538
UniProt ID	Q04656
Protein Families	Druggable Genome, Transmembrane
MW	154.8 kDa
Gene Summary	This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013]

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