Septin 2 (SEPT2) (NM_001282972) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DIFF6; hNedd5; NEDD-5; NEDD5; Pnutl3; SEPT2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237957 representing NM_001282972 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTGGATAAGCGAGGGGAGAGCGACTAGGCCCTGTCTGCGGGTACCTTCGGCGAGGAGAGGCGACG AAGGTCTGCACCAGCGAGACGAAGCTTCACAAAAGATGTCTAAGCAACAGCCAACTCAGTTTATAAATCC AGAAACACCTGGCTATGTTGGATTTGCAAACCTCCCCAATCAAGTTCACCGAAAATCAGTGAAAAAAGGT TTTGAGTTCACACTGATGGTGGTCGGTGAATCAGGTCTAGGAAAATCGACTCTCATAAACAGCCTATTCC TAACTGATCTGTACCCAGAAAGAGTCATACCTGGAGCAGCAGAAAAAATTGAAAGAACTGTCCAGATTGA GGCTTCAACTGTTGAAATTGAAGAGCGAGGGGTCAAGCTACGCCTGACAGTGGTAGATACCCCTGGCTAT GGTGACGCTATCAACTGCAGAGATTGTTTTAAGACAATTATCTCCTATATTGATGAGCAATTTGAGAGGT ACCTGCATGACGAGAGCGGCTTGAACAGGCGGCACATCATTGATAATAGGGTGCATTGTTGCTTTTACTT TATTTCACCTTTTGGACATGGACTTAAGCCCTTAGATGTGGCGTTTATGAAGGCAATACACAACAAGGTG AATATTGTGCCTGTCATTGCAAAAGCTGACACTCTCACCCTGAAGGAACGGGAGCGGCTGAAGAAAAGGA TTCTGGATGAAATTGAAGAACATAACATCAAAATCTATCACTTACCTGATGCAGAATCAGATGAAGATGA AGATTTTAAAGAGCAGACTAGACTTCTCAAGGCTAGCATCCCATTCTCTGTGGTTGGATCCAATCAGTTG ATTGAAGCCAAAGGAAAGAAGGTCAGAGGCCGCCTCTACCCCTGGGGTGTTGTGGAAGTGGAGAACCCAG AGCACAATGACTTTCTGAAGCTGAGAACCATGCTCATCACCCACATGCAGGATCTCCAGGAGGTGACCCA GGACCTTCATTATGAAAACTTCCGTTCTGAGAGACTCAAGAGAGGCGGCAGGAAAGTGGAGAATGAGGAC ATGAATAAAGACCAGATCTTGCTGGAAAAAGAAGCTGAGCTCCGCCGCATGCAAGAGATGATTGCAAGGA TGCAGGCGCAGATGCAGATGCAGATGCAGGGCGGGGATGGCGATGGCGGGGCTCTCGGGCACCACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237957 representing NM_001282972 Red=Cloning site Green=Tags(s) MPWISEGRATRPCLRVPSARRGDEGLHQRDEASQKMSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKG FEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGY GDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKV NIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQL IEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENED MNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282972
ORF Size	1188 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282972.1, NP_001269901.1
RefSeq Size	3457 bp
RefSeq ORF	1191 bp
Locus ID	4735
UniProt ID	Q15019
MW	45.9 kDa
Gene Summary	Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.[UniProtKB/Swiss-Prot Function]

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