CACNA1F (NM_001256789) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AIED; Cav1.4; Cav1.4alpha1; COD3; COD4; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; JM8; JMC8; OA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235352 representing NM_001256789 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGGCGGGAAAGACACCACCCCAGAGCCCAGTCCAGCCAATGGGGCAGGCCCTGGTC CCGAATGGGGGCTGTGCCCCGGGCCCCCAGCTGTGGAAGGTGAAAGCAGTGGGGCATCAGGCCTAGGGAC CCCTAAGCGAAGAAACCAGCACAGCAAGCACAAGACAGTGGCAGTGGCCAGTGCCCAGCGGTCACCTCGG GCACTCTTCTGCCTCACCCTGGCCAATCCTCTGCGACGGTCCTGCATCAGCATCGTGGAGTGGAAGCCCT TCGACATCCTCATCCTGCTGACCATCTTTGCCAACTGCGTGGCCCTGGGAGTTTACATCCCCTTCCCTGA GGACGACTCCAACACTGCCAACCACAACCTGGAGCAGGTGGAGTACGTATTCCTGGTGATTTTCACTGTG GAGACGGTGCTCAAGATCGTGGCCTACGGGCTGGTGCTCCACCCCAGCGCCTACATCCGCAATGGCTGGA ACCTACTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTTCTGCTGGAGCAGGGCCCCGGACGGCC AGGCGACGCCCCGCACACCGGGGGAAAGCCAGGAGGCTTCGATGTGAAGGCATTGAGGGCGTTTCGGGTG CTGCGGCCACTGAGGCTGGTGTCTGGGGTCCCGAGCCTGCACATAGTGCTCAATTCCATCATGAAGGCTC TGGTGCCGCTGCTGCACATTGCACTGCTCGTGCTCTTCGTCATCATCATTTATGCCATCATTGGGCTCGA GCTGTTCCTTGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAAGCGGAGGAGGACCCA TCGCCCTGTGCGTCTTCGGGATCAGGGCGTGCGTGCACGCTGAACCAGACTGAGTGCCGCGGGCGCTGGC CAGGGCCCAATGGAGGCATCACCAACTTTGACAACTTCTTCTTCGCCATGCTGACAGTCTTCCAGTGTGT CACCATGGAAGGCTGGACCGATGTGCTCTACTGGATGCAAGATGCCATGGGGTATGAACTGCCCTGGGTG TACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTCGTCCTCAACCTTGTGCTTGGCGTCCTGAGTGGGG AGTTCTCCAAGGAGAGAGAGAAAGCGAAAGCTCGCGGGGACTTCCAGAAGCAGCGGGAGAAGCAGCAGAT GGAGGAAGACCTGCGGGGCTACCTGGACTGGATCACTCAAGCCGAAGAGCTGGACATGGAGGACCCCTCC GCCGATGACAACCTTGGGCCACAGCTGGCCGAGCTGACCAATAGGAGGCGTGGACGTCTGCGCTGGTTCA GTCATTCTACTCGCTCCACACACTCCACCAGCAGCCATGCCAGCCTCCCAGCCAGTGACACCGGTTCCAT GACAGAGACCCAAGGCGATGAGGATGAGGAGGAGGGGGCTCTGGCCAGCTGTACACGCTGCCTAAACAAG ATCATGAAAACCAGAGTCTGCCGCCGCCTCCGCCGAGCCAACCGGGTCCTTCGGGCACGCTGCCGTCGGG CAGTGAAGTCCAATGCCTGCTACTGGGCTGTGCTGTTGCTCGTCTTCCTCAACACGTTGACCATCGCCTC TGAGCACCACGGGCAGCCTGTGTGGCTCACCCAGATCCAGGAGTATGCCAACAAAGTGTTGCTCTGTCTG TTCACGGTGGAGATGCTTCTCAAATTGTACGGTCTGGGCCCCTCTGCCTATGTGTCTTCCTTCTTCAACC GCTTTGACTGCTTTGTGGTCTGTGGGGGCATCCTAGAGACCACCTTGGTGGAGGTGGGTGCCATGCAGCC CTTGGGCATCTCAGTGCTCCGATGTGTGCGCCTCCTCAGGATCTTTAAGGTCACCAGACACTGGGCTTCT CTGAGCAATCTGGTGGCATCCCTGCTCAATTCAATGAAATCCATCGCATCCTTGCTGCTTCTCCTCTTCC TCTTCATCATTATCTTCTCCCTGCTTGGCATGCAGCTGTTTGGGGGCAAGTTCAACTTTGACCAGACCCA CACCAAGCGAAGCACCTTTGACACGTTCCCCCAGGCCCTCCTCACTGTCTTTCAGATCCTGACAGGTGAG GACTGGAACGTGGTCATGTATGATGGTATCATGGCATATGGTGGCCCCTTCTTCCCAGGAATGTTGGTGT GCATCTATTTCATCATTCTCTTCATCTGTGGCAACTACATCCTGTTGAACGTGTTTCTTGCCATTGCTGT GGACAACCTGGCCAGTGGAGATGCAGGCACTGCCAAGGACAAGGGCGGGGAGAAGAGCAATGAGAAGGAT CTCCCACAGGAGAATGAAGGCCTGGTGCCTGGTGTGGAGAAAGAGGAAGAGGAGGGTGCAAGGAGGGAAG GAGCAGACATGGAGGAGGAGGAGGAGGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAGGGTGCAGG GGGTGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAGAAGGTGGTACCCATCCCTGAGGGCAGCGCCTTC TTCTGCCTCAGCCAAACCAACCCGCTGAGGAAGGGCTGCCACACCCTCATCCACCATCATGTCTTCACCA ATCTTATCCTGGTGTTCATCATCCTCAGCAGTGTGTCCCTGGCCGCTGAGGACCCCATCCGAGCCCACTC CTTCCGCAACCATATTCTGGGTTACTTCGATTATGCCTTCACCTCCATTTTCACTGTGGAGATTCTACTA AAGATGACAGTGTTTGGGGCCTTCCTGCACCGCGGCTCCTTCTGCCGTAGCTGGTTTAATATGTTGGATC TGCTGGTGGTCAGTGTGTCCCTCATCTCCTTTGGCATCCACTCCAGCGCCATCTCGGTGGTGAAGATTCT GCGAGTACTCCGAGTACTGCGGCCCCTCCGAGCCATCAACAGGGCCAAGGGACTCAAGCATGTGGTGCAG TGTGTATTTGTGGCCATCCGGACCATCGGAAACATCATGATTGTCACCACACTTCTGCAATTTATGTTCG CCTGCATCGGGGTGCAGCTCTTCAAGGGGAAATTCTACACCTGCACGGACGAGGCCAAACACACCCCTCA AGAATGCAAGGGCTCCTTCCTGGTATACCCAGATGGAGACGTGTCACGGCCCCTGGTCCGGGAGCGGCTC TGGGTCAACAGTGATTTCAACTTTGACAATGTCCTTTCAGCCATGATGGCCCTGTTCACTGTCTCCACCT TTGAAGGCTGGCCTGCACTGCTATACAAGGCCATCGATGCATATGCAGAGGACCACGGCCCCATCTATAA TTACCGTGTGGAGATCTCAGTGTTCTTCATTGTCTACATCATCATCATTGCGTTCTTCATGATGAACATC TTCGTGGGCTTCGTCATCATCACTTTCCGTGCCCAGGGCGAGCAGGAGTACCAAAACTGTGAGCTGGACA AGAACCAGCGTCAATGTGTGGAATATGCCCTCAAGGCCCAGCCACTCCGCCGTTACATCCCCAAGAACCC GCATCAGTATCGTGTGTGGGCCACTGTGAACTCTGCTGCCTTTGAGTACCTGATGTTCCTGCTCATCCTG CTCAACACAGTTGCCCTAGCCATGCAGCACTATGAGCAGACTGCTCCCTTCAACTATGCCATGGACATCC TCAACATGGTCTTCACTGGCCTCTTCACTATTGAGATGGTGCTCAAAATCATCGCCTTCAAGCCCAAGCA TTACTTCACTGATGCCTGGAACACGTTTGACGCTCTTATTGTGGTGGGCAGCATAGTGGATATTGCCGTC ACTGAAGTCAATAATGGTGGCCACCTTGGCGAGAGCTCTGAGGACAGCTCCCGCATTTCCATTACCTTCT TTCGCCTCTTCCGAGTTATGCGGCTGGTCAAGCTTCTCAGTAAGGGTGAAGGGATCCGCACATTGCTCTG GACATTCATCAAGTCCTTCCAGGCCTTGCCCTATGTGGCTCTTCTCATCGCAATGATATTCTTCATCTAT GCCGTCATTGGCATGCAGATGTTCGGCAAGGTGGCTCTTCAGGATGGCACACAGATAAACCGAAACAACA ACTTCCAGACCTTTCCACAGGCTGTGCTGCTTCTGTTCAGGTGTGCCACTGGTGAGGCATGGCAGGAGAT AATGCTTGCCAGCCTTCCCGGAAATCGGTGTGATCCTGAGTCTGACTTCGGCCCTGGTGAAGAGTTTACC TGTGGTAGCAATTTTGCCATCGCCTATTTCATCAGCTTCTTCATGCTCTGTGCCTTCCTGATCATAAATC TCTTTGTGGCTGTGATCATGGACAACTTTGATTATCTCACCAGAGATTGGTCCATCCTGGGCCCCCATCA CCTTGATGAATTCAAGAGGATCTGGTCTGAATATGACCCTGGGGCCAAGGGCCGCATCAAACACTTGGAT GTGGTTGCCCTGCTGAGACGTATCCAGCCCCCTCTGGGATTTGGGAAGCTGTGCCCACACCGAGTGGCCT GCAAGAGACTTGTGGCAATGAACATGCCCCTCAACTCAGATGGGACGGTGACATTCAACGCCACACTCTT TGCCCTGGTCCGGACATCCCTGAAGATCAAAACAGAAGGGAACCTGGAGCAAGCCAACCAGGAGCTGCGG ATTGTCATCAAAAAGATCTGGAAGCGGATGAAACAGAAGCTGCTAGATGAGGTCATCCCCCCACCAGACG AGGAGGAGGTCACCGTGGGCAAATTCTACGCCACATTTCTGATCCAGGACTATTTCCGCAAATTCCGGCG GAGGAAAGAAAAAGGGCTACTAGGCAACGACGCCGCCCCTAGCACCTCTTCCGCCCTTCAGGCTGGTCTG CGGAGCCTGCAGGACTTGGGTCCTGAGATGCGGCAGGCCCTCACCTGTGACACAGAGGAGGAGGAAGAAG AGGGGCAGGAGGGAGTGGAGGAGGAAGATGAAAAGGACTTGGAAACTAACAAAGCCACGATGGTCTCCCA GCCCTCAGCTCGCCGGGGCTCCGGGATTTCTGTGTCTCTGCCTGTCGGGGACAGACTTCCAGATTCACTC TCCTTTGGGCCCAGTGATGATGACAGGGGGACTCCCACCTCCAGTCAGCCCAGTGTGCCCCAGGCTGGAT CCAACACCCACAGGAGAGGCTCTGGGGCTCTCATTTTCACCATCCCAGAAGAAGGAAATTCTCAGCCCAA GGGAACCAAAGGGCAAAACAAGCAAGATGAGGATGAGGAAGTCCCTGATCGGCTTTCCTACCTAGATGAG CAGGCAGGGACTCCCCCGTGCTCAGTCCTTTTGCCACCTCACAGAGCTCAGAGATACATGGATGGGCACC TGGTACCACGCCGCCGTCTGCTGCCCCCCACACCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCT GCAGCGCCAGGGCAGTTGTGAGGATTTACCCATCCCAGGCACCTATCATCGTGGGCGAAATTCAGGGCCC AATAGGGCTCAGGGTTCCTGGGCAACACCACCTCAGCGGGGTCGGCTCCTGTATGCCCCGCTGTTGTTGG TGGAAGAGGGCGCAGCGGGGGAGGGGTACCTCGGCAGATCCAGTGGCCCACTGCGCACCTTCACCTGTCT GCACGTGCCTGGAACCCACTCGGACCCCAGCCATGGGAAGAGGGGCAGTGCCGACAGCTTGGTGGAGGCT GTGCTTATCTCAGAGGGTCTGGGCCTCTTTGCTCGAGACCCACGTTTCGTGGCCCTGGCCAAGCAGGAGA TTGCAGATGCGTGTCGCCTGACGCTGGATGAGATGGACAATGCTGCCAGTGACCTGCTGGCACAGGGAAC CAGCTCTCTCTATAGCGACGAGGAGTCCATCCTCTCCCGCTTCGATGAGGAGGACTTGGGAGACGAGATG GCCTGCGTCCACGCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235352 representing NM_001256789 Red=Cloning site Green=Tags(s) MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPR ALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTV ETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRV LRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDP SPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWV YFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPS ADDNLGPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEGALASCTRCLNK IMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQIQEYANKVLLCL FTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWAS LSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGE DWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGGEKSNEKD LPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKEKVVPIPEGSAF FCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILL KMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQ CVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERL WVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVYIIIIAFFMMNI FVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLIL LNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAV TEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIY AVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFT CGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLD VVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELR IVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAAPSTSSALQAGL RSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVSLPVGDRLPDSL SFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDEEVPDRLSYLDE QAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIPGTYHRGRNSGP NRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHGKRGSADSLVEA VLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILSRFDEEDLGDEM ACVHAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256789
ORF Size	5898 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256789.3
RefSeq Size	6037 bp
RefSeq ORF	5901 bp
Locus ID	778
UniProt ID	O60840
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
MW	219.9 kDa
Gene Summary	This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013]

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