SETMAR (NM_001276325) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mar1; METNASE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233886 representing NM_001276325 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCGGAAGCGGCAAAGACGACACGGCCTTGTGGGATGGCGGAGTTTAAGGAGAAGCCTGAGGCCC CGACTGAGCAGCTGGATGTCGCGTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCCGC GCCGGCGCCCTTCCAGTACACTCCTGATCATGTAGTTGGACCTGGAGCAGACATTGATCCCACTCAAATA ACCTTTCCCGGATGCATTTGTGTCAAAACTCCCTGCCTCCCTGGCACTTGCTCCTGTCTCCGCCATGGAG AGAACTATGATGATAACTCATGCCTTAGAGATATAGGATCTGGAGGAAAGTATGCAGAGCCTGTTTTTGA ATGCAATGTCCTGTGCCGATGCAGTGACCACTGCAGAAACAGAGTGGTCCAGAAAGGTCTACAGTTCCAC TTCCAAGTGTTCAAGACGCATAAAAAAGGCTGGGGACTTCGTACCTTGGAATTTATACCGAAAGGAAGGT TTGTCTGTGAATATGCTGGTGAGGTTTTAGGATTCTCTGAAGTTCAGAGAAGAATTCACTTACAAACAAA ATCCGACTCCAATTACATTATAGCCATCAGGGAACATGTTTATAATGGGCAGGTAATGGAAACATTTGTT GACCCTACTTATATAGGAAATATTGGAAGATTCCTTAATCATTCTTGTGAGCCAAACCTTTTGATGATTC CTGTCCGAATTGACTCAATGGTACCTAAGTTGGCACTTTTTGCAGCCAAAGATATTGTGCCAGAAGAAGA ACTCTCTTATGATTATTCAGGAAGATATCTTAATCTAACAGTCAGTGAAGACAAAGAAAGGCTAGATCAT GGGAAACTAAGGAAACCTTGTTACTGTGGTGCCAAATCATGTACTGCTTTCCTGCCTTTTGACAGTTCTC TGTACTGCCCCGTAGAAAAGTCGAACATCAGTTGTGGAAATGAGAAGGAACCCAGCATGTGTGGCTCAGC CCCTTCTGTGTTCCCCTCCTGCAAGCGATTGACCCTTGAGGTGAGTCTGTTCAGTGATAAGCAGCTTGCC CCTCCCTATAGTGGAAGACAGTGGTTGGCTAGCTTTACCTCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233886 representing NM_001276325 Red=Cloning site Green=Tags(s) MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQI TFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFH FQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFV DPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDH GKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLEVSLFSDKQLA PPYSGRQWLASFTSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276325
ORF Size	1095 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276325.1, NP_001263254.1
RefSeq Size	1775 bp
RefSeq ORF	1098 bp
Locus ID	6419
UniProt ID	Q53H47
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Lysine degradation
MW	41 kDa
Gene Summary	This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

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