GPRASP1 (NM_001184727) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GASP; GASP-1; GASP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC230712 representing NM_001184727 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGGGGCAGAGATTGAGTCTGGTGCCCAGGTCAAGCCTGAAAAGAAGCCTGGGGAAGAGGTTGTAG GTGGGGCTGAGATAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCCCAGATAAT GCCTGGGGCAAGGCCCAAGAATAAGTCCAAGGTTATGCCTGGAGCAAGCACCAAAGTTGAGACAAGTGCA GTGGGTGGGGCACGCCCTAAGAGTAAGGCCAAGGCAATACCTGTTTCACGATTTAAGGAAGAAGCCCAGA TGTGGGCTCAGCCCAGGTTTGGTGCTGAAAGATTGTCTAAGACAGAGAGAAACTCCCAGACCAATATCAT AGCCTCTCCACTTGTCAGTACTGATTCTGTCTTGGTTGCTAAAACAAAGTACCTGTCTGAGGATAGAGAA CTGGTTAATACAGACACTGAGAGCTTTCCTAGAAGGAAGGCCCATTACCAAGCAGGATTCCAGCCTTCTT TTAGGTCAAAGGAGGAGACCAATATGGGGTCCTGGTGCTGTCCTAGGCCTACATCCAAACAAGAAGCCTC TCCTAATTCTGATTTCAAATGGGTAGACAAATCTGTGAGTTCCTTGTTCTGGAGTGGAGATGAGGTCACT GCAAAATTTCATCCTGGGAATAGGGTAAAAGACAGTAACAGATCCATGCACATGGCCAATCAAGAGGCTA ATACCATGTCTAGGTCCCAAACTAACCAGGAGCTCTATATTGCATCTAGTTCTGGTTCTGAGGATGAGTC TGTTAAGACACCCTGGTTCTGGGCCAGAGATAAAACCAATACCTGGTCTGGGCCCAGGGAAGATCCCAAT AGCAGGTCCAGGTTTAGGTCTAAGAAAGAAGTCTATGTTGAATCAAGTTCTGGATCTGAGCATGAAGACC ATTTGGAGTCCTGGTTTGGGGCTGGAAAGGAGGCCAAATTCAGGTCCAAAATGAGAGCTGGGAAGGAGGC CAATAACAGGGCCAGGCACAGGGCCAAGCGAGAAGCTTGCATTGATTTCATGCCTGGGTCTATAGATGTA ATTAAAAAAGAGTCCTGTTTCTGGCCTGAAGAAAATGCTAATACCTTTTCAAGGCCCATGATCAAGAAAG AGGCCAGGGCCAGAGCAATGACAAAGGAAGAGGCCAAAACCAAGGCCCGAGCCAGGGCCAAGCAAGAAGC CAGGTCAGAGGAGGAAGCCCTCATTGGGACCTGGTTCTGGGCTACAGACGAGTCCAGCATGGCAGATGAA GCCAGCATAGAGTCCAGTCTACAAGTGGAGGATGAGTCCATAATTGGGAGTTGGTTCTGGACTGAAGAAG AGGCCAGTATGGGGACTGGGGCTAGCAGTAAATCCAGACCAAGGACTGATGGGGAGCGTATTGGTGATTC CTTATTTGGGGCTAGGGAAAAGACCAGTATGAAAACTGGGGCTGAGGCCACCTCTGAATCTATACTAGCA GCTGATGATGAACAGGTCATTATTGGTTCCTGGTTCTGGGCTGGTGAAGAGGTCAACCAAGAGGCTGAGG AAGAGACCATTTTTGGGTCGTGGTTCTGGGTCATTGATGCGGCCAGTGTGGAATCTGGTGTTGGGGTCAG CTGTGAGTCCAGGACAAGGTCTGAGGAAGAAGAGGTCATTGGTCCCTGGTTTTGGTCTGGAGAACAAGTT GATATAGAGGCTGGAATCGGAGAAGAGGCCAGGCCAGGAGCTGAAGAAGAGACAATATTCGGGTCCTGGT TTTGGGCTGAAAACCAGACCTATATGGATTGTAGGGCTGAAACTAGCTGTGACACCATGCAAGGGGCTGA GGAGGAGGAGCCCATTATTGGGTCCTGGTTTTGGACCAGAGTAGAAGCTTGTGTGGAGGGTGATGTCAAC AGCAAGTCTAGCCTGGAGGACAAGGAAGAGGCCATGATACCATGTTTTGGAGCCAAAGAAGAGGTCAGTA TGAAGCATGGGACTGGTGTCAGATGCAGATTTATGGCAGGGGCTGAGGAGACCAATAATAAGTCTTGCTT CTGGGCAGAAAAAGAACCCTGTATGTATCCTGCCGGTGGAGGAAGTTGGAAGTCTAGGCCAGAGGAGGAA GAGGACATTGTCAATTCGTGGTTCTGGTCCAGAAAATACACAAAGCCAGAGGCCATTATAGGGTCCTGGT TATGGGCTACAGAAGAGAGTAATATAGATGGGACTGGAGAAAAGGCCAAGTTACTGACTGAAGAGGAGAC CATAATCAATTCCTGGTTCTGGAAAGAAGATGAAGCCATTTCAGAGGCTACTGACAGAGAAGAGTCCAGG CCAGAAGCTGAGGAGGGGGACATTATTGGTTCTTGGTTCTGGGCTGGAGAAGAGGACAGACTAGAGCCAG CTGCTGAGACTAGAGAAGAAGACAGGCTAGCAGCTGAGAAAGAAGGTATTGTTGGGTCCTGGTTTGGGGC CAGAGAAGAGACCATTAGAAGAGAGGCTGGGTCTTGCAGCAAATCCAGTCCTAAAGCTGAAGAGGAAGAA GTCATTATTGGGTCCTGGTTCTGGGAAGAAGAGGCCAGTCCGGAGGCAGTGGCAGGAGTCGGCTTTGAGT CAAAGCCTGGGACTGAGGAGGAAGAAATCACTGTTGGGTCCTGGTTCTGGCCTGAAGAAGAAGCCAGTAT ACAGGCTGGATCTCAGGCAGTAGAGGAAATGGAGTCAGAGACTGAAGAGGAAACCATTTTTGGGTCCTGG TTCTGGGATGGAAAAGAAGTCAGTGAAGAAGCAGGACCATGCTGTGTATCCAAGCCAGAGGATGATGAAG AGATGATTGTTGAGTCCTGGTTCTGGTCTAGAGACAAAGCCATTAAGGAAACTGGAACTGTGGCCACCTG TGAGTCCAAGCCAGAAAATGAGGAAGGGGCCATTGTTGGGTCTTGGTTTGAGGCTGAAGATGAGGTAGAT AACAGGACTGACAATGGAAGCAACTGTGGGTCCAGGACATTAGCTGATGAAGATGAGGCCATAGTGGGGT CCTGGTTCTGGGCAGGAGATGAGGCCCATTTTGAATCAAATCCTAGCCCCGTGTTCAGGGCCATTTGCAG GTCCACGTGTTCAGTTGAACAGGAGCCTGATCCTTCACGCAGGCCTCAGAGTTGGGAGGAGGTCACTGTT CAGTTCAAGCCTGGTCCATGGGGTAGGGTCGGCTTCCCATCTATAAGCCCCTTTAGATTTCCGAAAGAGG CAGCATCTTTATTCTGTGAAATGTTTGGGGGCAAACCCAGGAACATGGTACTTAGCCCAGAAGGGGAAGA TCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCCCATTTCAGTATGATCCTTCCTACAGG TCAGTCCAGGAAATTCGAGAGCATCTTAGGGCCAAGGAGAGTACAGAGCCTGAGAGTTCATCCTGTAACT GCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAACTCCTTTTATTAATGGAAAAAATTCG GGATCCTTTTATTCATGAAATATCTAAAATCGCAATGGGTATGAGAAGTGCTTCTCAATTTACCCGAGAT TTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCGTCCTCCCGAGTTAGAA CAAGTTTTTTGGAAAATATGATTCGCATGGCCCCACCTTATCCGAATCTAAACATAATTCAGACATACAT ATGTAAAGTGTGTGAGGAAACCCTTGCTTATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATG ATTAGACATCTCACTACTACTACTGACTATCACACACTGGTTGCCAATTATATGTCTGGGTTTCTCTCCT TATTAGCTACAGGCAATGCCAAAACAAGGTTTCATGTTTTGAAAATGCTACTGAATTTGTCTGAAAATCT TTTCATGACAAAAGAACTACTCAGTGCTGAAGCAGTGTCAGAATTTATAGGCCTCTTTAACAGGGAAGAG ACAAATGACAATATTCAAATTGTTCTTGCAATATTTGAGAATATTGGCAACAATATCAAAAAAGAAACAG TGTTCTCTGATGATGATTTCAATATTGAGCCGCTTATTTCTGCATTCCACAAAGTTGAGAAATTTGCTAA GGAACTGCAAGGCAAAACAGACAATCAAAATGACCCTGAAGGGGACCAAGAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC230712 representing NM_001184727 Red=Cloning site Green=Tags(s) MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSA VGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRE LVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVT AKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPN SRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDV IKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADE ASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILA ADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQV DIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVN SKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEE EDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESR PEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEE VIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSW FWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVD NRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTV QFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYR SVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRD FIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRM IRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREE TNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001184727
ORF Size	4185 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001184727.2
RefSeq ORF	4188 bp
Locus ID	9737
UniProt ID	Q5JY77
Protein Families	Druggable Genome
MW	157.3 kDa
Gene Summary	This gene encodes a member of the GPRASP (G protein-coupled receptor associated sorting protein) family. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R). Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.