United States
Germany
Japan
United Kingdom
China
DHX36 (NM_001114397) Human Tagged ORF Clone
CAT＃: RC226291
- TrueORF®
DHX36 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 2
ORF Plasmid: tGFP
"NM_001114397" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DDX36; G4R1; MLEL1; RHAU |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226291 representing NM_001114397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTATGACTACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGTGGGGGCTATGGAG GGGGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTCCGGAGGAGGCGGCGGCGGCGGAGGGGGTGGTCG AGGCGGCAGGGGCCGGCATCCCGGGCACCTGAAAGGCCGCGAAATCGGCATGTGGTACGCGAAAAAACAG GGGCAGAAGAACAAGGAAGCGGAGAGGCAAGAGAGAGCTGTAGTACACATGGATGAACGACGAGAAGAAC AAATTGTACAGTTACTGAATTCTGTTCAAGCGAAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTT TGCTCCTGAGGATCATGGATACGGTACTGAAGTTTCTACTAAGAACACACCATGCTCAGAGAACAAACTT GACATCCAGGAAAAGAAGTTGATAAATCAAGAAAAAAAAATGTTTAGAATCAGGAACAGATCATATATTG ACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAGATGGAACTTTAGACCAAAAATTATTGGAAGA TTTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATGCAGCATTTCAGAGAAAAGCTGCCTTCGTAT GGAATGCAAAAGGAATTGGTAAATTTAATTGATAACCATCAGGTAACAGTAATAAGTGGTGAAACTGGTT GTGGCAAAACCACTCAAGTTACTCAGTTCATTTTGGATAACTACATTGAAAGAGGAAAAGGATCTGCTTG CAGAATAGTTTGTACTCAGCCAAGAAGAATTAGTGCCATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAGG GCAGAATCTTGTGGCAGTGGTAATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTGCCAAGGAAAC AGGGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTCCAGTCAGACCCGTATTTGTCCAG TGTTAGTCATATCGTACTTGATGAAATCCATGAAAGAAATCTGCAGTCAGATGTTTTAATGACTGTTGTT AAAGACCTTCTCAATTTTCGATCTGACTTGAAAGTAATATTGATGAGTGCAACATTGAATGCAGAAAAGT TTTCAGAATATTTTGGTAACTGTCCAATGATACATATACCTGGTTTTACCTTTCCGGTTGTGGAATATCT TTTGGAAGATGTAATTGAAAAAATAAGGTATGTTCCAGAACAAAAAGAACACAGATCCCAGTTTAAGAGG GGTTTCATGCAAGGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGAAGCAATATATAAAGAACGTTGGC CAGATTATGTAAGGGAACTGCGAAGAAGGTATTCTGCAAGTACTGTAGATGTTATAGAAATGATGGAGGA TGATAAAGTTGATCTGAATTTGATTGTTGCCCTCATCCGATACATTGTTTTGGAAGAAGAGGATGGTGCG ATACTGGTCTTTCTGCCAGGCTGGGACAATATCAGCACTTTACATGATCTCTTGATGTCACAAGTAATGT TTAAATCAGTTAACCAGACACAGGTGTTTAAAAGAACCCCTCCTGGTGTTCGGAAAATAGTAATTGCTAC CAACATTGCGGAGACTAGCATTACCATAGATGATGTCGTTTATGTGATAGATGGAGGAAAAATAAAAGAG ACGCATTTTGATACTCAGAACAATATCAGTACAATGTCCGCTGAGTGGGTTAGTAAAGCTAATGCCAAAC AGAGAAAAGGTCGAGCTGGAAGAGTTCAACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGAGCAAG TCTTCTAGATGACTATCAACTGCCAGAAATTTTGAGAACTCCTTTGGAAGAACTTTGTTTACAAATAAAG ATTTTAAGGCTAGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGACCCACCATCAAATGAGGCAGTGT TACTCTCCATAAGACACCTGATGGAGCTGAACGCTTTGGATAAACAAGAAGAATTGACACCTCTTGGAGT CCACTTGGCACGATTACCCGTTGAGCCACATATTGGAAAAATGATTCTTTTTGGAGCACTGTTCTGCTGC TTAGACCCAGTACTCACTATTGCTGCTAGTCTCAGTTTCAAAGATCCATTTGTCATTCCACTGGGAAAAG AAAAGATTGCAGATGCAAGAAGAAAGGAATTGGCAAAGGATACTAGAAGTGATCACTTAACAGTTGTGAA TGCGTTTGAGGGCTGGGAAGAGGCTAGGCGACGTGGTTTCAGATACGAAAAGGACTATTGCTGGGAATAT TTTCTGTCTTCAAACACACTGCAGATGCTGCATAACATGAAAGGACAGTTTGCTGAGCATCTTCTTGGAG CTGGATTTGTAAGCAGTAGAAATCCTAAAGATCCAGAATCTAATATAAATTCAGATAATGAGAAGATAAT TAAAGCTGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATTCGACTAAATTTGGGTAAAAAAAGA AAAATGGTAAAAGTTTACACAAAAACCGATGGCCTGGTTGCTGTTCATCCTAAATCTGTTAATGTGGAGC AAACAGACTTTCACTACAACTGGCTTATCTATCACCTAAAGATGAGAACAAGCAGTATATACTTGTATGA CTGCACAGAGGTTTCCCCATACTGTCTCTTGTTTTTTGGAGGTGACATTTCCATCCAGAAGGATAACGAT CAGGAAACTATTGCTGTAGATGAGTGGATTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGG AATTAAGAAAGGAACTAGATATTCTTCTGCAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGA CACTAAATCCAGAGACTGTGCAGTACTGTCAGCTATTATAGACTTGATCAAAACACAGGAAAAGGCAACT CCCAGGAACTTTCCGCCACGATTCCAGGATGGATATTACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226291 representing NM_001114397
Red=Cloning site Green=Tags(s) MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQ GQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKL DIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSY GMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAER AESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVV KDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKR GFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGA ILVFLPGWDNISTLHDLLMSQVMFKSVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKE THFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIK ILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCC LDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEY FLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKR KMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDND QETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKAT PRNFPPRFQDGYYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114397 |
| ORF Size | 2982 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114397.1, NP_001107869.1 |
| RefSeq ORF | 2985 bp |
| Locus ID | 170506 |
| UniProt ID | Q9H2U1 |
| MW | 113 kDa |
| Gene Summary | This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226291L3 | Lenti-ORF clone of DHX36 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 2 |
CNY 9,030.00 |
|
| RC226291L4 | Lenti-ORF clone of DHX36 (mGFP-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 2 |
CNY 9,030.00 |
|
| RG226291 | DHX36 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 2 |
CNY 7,890.00 |
|
| SC319014 | DHX36 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 2 |
CNY 19,100.00 |
