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TMIE (NM_147196) Human Tagged ORF Clone
CAT＃: RC220050
TMIE (Myc-DDK-tagged)-Human transmembrane inner ear (TMIE)
ORF Plasmid: tGFP
"NM_147196" in other vectors (7)
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CNY 1,200.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNB6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220050 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGTGGCCGGGCGCGGGTCCCCTCTGCGTGCTGGGCGGCGCCGCACTCGGGGTGTGCCTCGCGG GGGTTGCCGGGCAGCTGGTGGAGCCCAGCACGGCCCCACCCAAGCCCAAGCCGCCTCCGCTGACCAAGGA GACAGTGGTGTTCTGGGACATGCGCCTGTGGCACGTGGTGGGCATCTTTTCGCTCTTCGTGTTGTCCATC ATCATCACGCTGTGCTGTGTCTTCAACTGTCGTGTGCCACGGACCCGGAAGGAGATCGAAGCCCGGTACC TGCAGCGAAAGGCAGCCAAGATGTACACAGACAAGCTGGAGACTGTGCCACCCCTCAATGAGCTCACAGA AGTCCCAGGAGAGGATAAGAAGAAGAAGAAGAAGAAGAAGGACAGTGTGGACACAGTGGCCATCAAAGTA GAGGAGGATGAGAAGAATGAGGCCAAGAAGAAGAAAGGAGAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220050 protein sequence
Red=Cloning site Green=Tags(s) MAGWPGAGPLCVLGGAALGVCLAGVAGQLVEPSTAPPKPKPPPLTKETVVFWDMRLWHVVGIFSLFVLSI IITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKDSVDTVAIKV EEDEKNEAKKKKGEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147196 |
| ORF Size | 465 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147196.1 |
| RefSeq Size | 1861 bp |
| RefSeq ORF | 471 bp |
| Locus ID | 259236 |
| UniProt ID | Q8NEW7 |
| Protein Families | Transmembrane |
| MW | 17.1 kDa |
| Gene Summary | This gene encodes a transmembrane inner ear protein. Studies in mouse suggest that this gene is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles. This gene is one of multiple genes responsible for recessive non-syndromic deafness (DFNB), also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220050L1 | Lenti ORF clone of Human transmembrane inner ear (TMIE), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC220050L2 | Lenti ORF clone of Human transmembrane inner ear (TMIE), mGFP tagged |
CNY 5,890.00 |
|
| RC220050L3 | Lenti ORF clone of Human transmembrane inner ear (TMIE), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220050L4 | Lenti ORF clone of Human transmembrane inner ear (TMIE), mGFP tagged |
CNY 5,890.00 |
|
| RG220050 | TMIE (tGFP-tagged) - Human transmembrane inner ear (TMIE) |
CNY 2,800.00 |
|
| SC306309 | TMIE (untagged)-Human transmembrane inner ear (TMIE) |
CNY 3,990.00 |
|
| SC317254 | TMIE (untagged)-Human transmembrane inner ear (TMIE) |
CNY 1,200.00 |
