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TRPC1 (NM_003304) Human Tagged ORF Clone
CAT＃: RC219662
- TrueORF®
TRPC1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1)
ORF Plasmid: tGFP
"NM_003304" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HTRP-1; TRP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219662 representing NM_003304
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGGCCCTGTACCCGAGCACGGACCTCTCGGGCGCCTCCTCCTCCTCCCTGCCTTCCTCTCCAT CCTCTTCCTCGCCGAACGAGGTGATGGCGCTGAAGGATGTGCGGGAGGTGAAGGAGGAGAATACGCTGAA TGAGAAGCTTTTCTTGCTGGCGTGCGACAAGGGTGACTATTATATGGTTAAAAAGATTTTGGAGGAAAAC AGTTCAGGTGACTTGAACATAAATTGCGTAGATGTGCTTGGGAGAAATGCTGTTACCATAACTATTGAAA ACGAAAACTTGGATATACTGCAGCTTCTTTTGGACTACGGTTGTCAGAAACTAATGGAACGAATTCAGAA TCCTGAGTATTCAACAACTATGGATGTTGCACCTGTCATTTTAGCTGCTCATCGTAACAACTATGAAATT CTTACAATGCTCTTAAAACAGGATGTATCTCTACCCAAGCCCCATGCAGTTGGCTGTGAATGCACATTGT GTTCTGCAAAAAACAAAAAGGATAGCCTCCGGCATTCCAGGTTTCGTCTTGATATATATCGATGTTTGGC CAGTCCAGCTCTAATAATGTTAACAGAGGAGGATCCAATTCTGAGAGCATTTGAACTTAGTGCTGATTTA AAAGAACTAAGTCTTGTGGAGGTGGAATTCAGGAATGATTATGAGGAACTAGCCCGGCAATGTAAAATGT TTGCTAAGGATTTACTTGCACAAGCCCGGAATTCTCGTGAATTGGAAGTTATTCTAAACCATACGTCTAG TGACGAGCCTCTTGACAAACGGGGATTATTAGAAGAAAGAATGAATTTAAGTCGTCTAAAACTTGCTATC AAATATAACCAGAAAGAGTTTGTCTCCCAGTCTAACTGCCAGCAGTTCCTGAACACTGTTTGGTTTGGAC AGATGTCGGGTTACCGACGCAAGCCCACCTGTAAGAAGATAATGACTGTTTTGACAGTAGGCATCTTTTG GCCAGTTTTGTCACTTTGTTATTTGATAGCTCCCAAATCTCAGTTTGGCAGAATCATTCACACACCTTTT ATGAAATTTATCATTCATGGAGCATCATATTTCACATTTCTGCTGTTGCTTAATCTATACTCTCTTGTCT ACAATGAGGATAAGAAAAACACAATGGGGCCAGCCCTTGAAAGAATAGACTATCTTCTTATTCTGTGGAT TATTGGGATGATTTGGTCAGACATTAAAAGACTCTGGTATGAAGGGTTGGAAGACTTTTTAGAAGAATCT CGTAATCAACTCAGTTTTGTCATGAATTCTCTTTATTTGGCAACCTTTGCCCTCAAAGTGGTTGCTCACA ACAAGTTTCATGATTTTGCTGATCGGAAGGATTGGGATGCATTCCATCCTACACTGGTGGCAGAAGGGCT TTTTGCATTTGCAAATGTTCTAAGTTATCTTCGTCTCTTTTTTATGTATACAACCAGCTCTATCTTGGGT CCATTACAGATTTCAATGGGACAGATGTTACAAGATTTTGGAAAATTTCTTGGGATGTTTCTTCTTGTTT TGTTTTCTTTCACAATTGGACTGACACAACTGTATGATAAAGGATATACTTCAAAGGAGCAGAAGGACTG TGTAGGCATCTTCTGTGAACAGCAAAGCAATGATACCTTCCATTCGTTCATTGGCACCTGCTTTGCTTTG TTCTGGTATATTTTCTCCTTAGCGCATGTGGCAATCTTTGTCACAAGATTTAGCTATGGAGAAGAACTGC AGTCCTTTGTGGGAGCTGTCATTGTTGGTACATACAATGTCGTGGTTGTGATTGTGCTTACCAAACTGCT GGTGGCAATGCTTCATAAAAGCTTTCAGTTGATAGCAAATCATGAAGACAAAGAATGGAAGTTTGCTCGA GCAAAATTATGGCTTAGCTACTTTGATGACAAATGTACGTTACCTCCACCTTTCAACATCATTCCCTCAC CAAAGACTATCTGCTATATGATTAGTAGCCTCAGTAAGTGGATTTGCTCTCATACATCAAAAGGCAAGGT CAAACGGCAAAACAGTTTAAAGGAATGGAGGAATTTGAAACAGAAGAGAGATGAAAACTATCAAAAAGTG ATGTGCTGCCTAGTGCATCGTTACTTGACTTCCATGAGACAGAAGATGCAAAGTACAGATCAGGCAACTG TGGAAAATCTAAACGAACTGCGCCAAGATCTGTCAAAATTCCGAAATGAAATAAGGGATTTACTTGGCTT TCGGACTTCTAAATATGCTATGTTTTATCCAAGAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219662 representing NM_003304
Red=Cloning site Green=Tags(s) MMAALYPSTDLSGASSSSLPSSPSSSSPNEVMALKDVREVKEENTLNEKLFLLACDKGDYYMVKKILEEN SSGDLNINCVDVLGRNAVTITIENENLDILQLLLDYGCQKLMERIQNPEYSTTMDVAPVILAAHRNNYEI LTMLLKQDVSLPKPHAVGCECTLCSAKNKKDSLRHSRFRLDIYRCLASPALIMLTEEDPILRAFELSADL KELSLVEVEFRNDYEELARQCKMFAKDLLAQARNSRELEVILNHTSSDEPLDKRGLLEERMNLSRLKLAI KYNQKEFVSQSNCQQFLNTVWFGQMSGYRRKPTCKKIMTVLTVGIFWPVLSLCYLIAPKSQFGRIIHTPF MKFIIHGASYFTFLLLLNLYSLVYNEDKKNTMGPALERIDYLLILWIIGMIWSDIKRLWYEGLEDFLEES RNQLSFVMNSLYLATFALKVVAHNKFHDFADRKDWDAFHPTLVAEGLFAFANVLSYLRLFFMYTTSSILG PLQISMGQMLQDFGKFLGMFLLVLFSFTIGLTQLYDKGYTSKEQKDCVGIFCEQQSNDTFHSFIGTCFAL FWYIFSLAHVAIFVTRFSYGEELQSFVGAVIVGTYNVVVVIVLTKLLVAMLHKSFQLIANHEDKEWKFAR AKLWLSYFDDKCTLPPPFNIIPSPKTICYMISSLSKWICSHTSKGKVKRQNSLKEWRNLKQKRDENYQKV MCCLVHRYLTSMRQKMQSTDQATVENLNELRQDLSKFRNEIRDLLGFRTSKYAMFYPRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003304 |
| ORF Size | 2277 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003304.4 |
| RefSeq Size | 4085 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 7220 |
| UniProt ID | P48995 |
| Domains | ANK, ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Huntington's disease, Parkinson's disease |
| MW | 87.4 kDa |
| Gene Summary | The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219662L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1), Myc-DDK-tagged |
CNY 10,736.00 |
|
| RC219662L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1), mGFP tagged |
CNY 10,736.00 |
|
| RC219662L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1), Myc-DDK-tagged |
CNY 10,736.00 |
|
| RC219662L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1), mGFP tagged |
CNY 10,736.00 |
|
| RG219662 | TRPC1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1) |
CNY 9,936.00 |
|
| SC121934 | TRPC1 (untagged)-Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1) |
CNY 8,352.00 |
