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KDM6B (NM_001080424) Human Tagged ORF Clone
CAT＃: RC219461
- TrueORF®
KDM6B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 6B (KDM6B)
ORF Plasmid: tGFP
"NM_001080424" in other vectors (4)
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CNY 16,960.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JMJD3; NEDCFSA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219461 representing NM_001080424
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCGCTGCACGGGAAGCCTTTGCCCTTGGGGGCCTGAGCT GTGCTGGGGCCTGGAGCTCCTGCCCGCCTCATCCCCCTCCTCGTAGCGCATGGCTGCCTGGAGGCAGATG CTCAGCCAGCATTGGGCAGCCCCCGCTTCCTGCTCCCCTACCCCCTTCACATGGCAGTAGTTCTGGGCAC CCCAGCAAACCATATTATGCTCCAGGGGCGCCCACTCCAAGACCCCTCCATGGGAAGCTGGAATCCCTGC ATGGCTGTGTGCAGGCATTGCTCCGGGAGCCAGCCCAGCCAGGGCTTTGGGAACAGCTTGGGCAACTGTA CGAGTCAGAGCACGATAGTGAGGAGGCCACACGCTGCTACCACAGCGCCCTTCGATACGGAGGAAGCTTC GCTGAGCTGGGGCCCCGCATTGGCCGACTGCAGCAGGCCCAGCTCTGGAACTTTCATACTGGCTCCTGCC AGCACCGAGCCAAGGTCCTGCCCCCACTGGAGCAAGTGTGGAACTTGCTACACCTTGAGCACAAACGGAA CTATGGAGCCAAGCGGGGAGGTCCCCCGGTGAAGCGAGCTGCTGAACCCCCAGTGGTGCAGCCTGTGCCT CCTGCAGCACTCTCAGGCCCCTCAGGGGAGGAGGGCCTCAGCCCTGGAGGCAAGCGAAGGAGAGGCTGCA ACTCTGAACAGACTGGCCTTCCCCCAGGGCTGCCACTGCCTCCACCACCATTACCACCACCACCACCACC ACCACCACCACCACCACCACCCCTGCCTGGCCTGGCTACCAGCCCCCCATTTCAGCTAACCAAGCCAGGG CTGTGGAGTACCCTGCATGGAGATGCCTGGGGCCCAGAGCGCAAGGGTTCAGCACCCCCAGAGCGCCAGG AGCAGCGGCACTCGCTGCCTCACCCATATCCATACCCAGCTCCAGCGTACACCGCGCACCCCCCTGGCCA CCGGCTGGTCCCGGCTGCTCCCCCAGGCCCAGGCCCCCGCCCCCCAGGAGCAGAGAGCCATGGCTGCCTG CCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACTCCAGCG TTTCACCAGCAGCAACCACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGGCACCAC CACCAGCAGCAGCAGTAGCAGCAGCAGCAACACTGGTCTCCGGGGCGTGGAGCCGAACCCAGGCATTCCC GGCGCTGACCATTACCAAACTCCCGCGCTGGAGGTCTCTCACCATGGCCGCCTGGGGCCCTCGGCACACA GCAGTCGGAAACCGTTCTTGGGGGCTCCCGCTGCCACTCCCCACCTATCCCTGCCACCTGGACCTTCCTC ACCCCCTCCACCCCCCTGTCCCCGCCTCTTACGCCCCCCACCACCCCCTGCCTGGTTGAAGGGTCCGGCC TGCCGGGCAGCCCGAGAGGATGGAGAGATCTTAGAAGAGCTCTTCTTTGGGACTGAGGGACCCCCCCGCC CTGCCCCACCACCCCTCCCCCATCGCGAGGGCTTCTTGGGGCCTCCGGCCTCCCGCTTTTCTGTGGGCAC TCAGGATTCTCACACCCCTCCCACTCCCCCAACCCCAACCACCAGCAGTAGCAACAGCAACAGTGGCAGC CACAGCAGCAGCCCTGCTGGGCCTGTGTCCTTTCCCCCACCACCCTATCTGGCCAGAAGTATAGACCCCC TTCCCCGGCCTCCCAGCCCAGCACAGAACCCCCAGGACCCACCTCTTGTACCCCTGACTCTTGCCCTGCC TCCAGCCCCTCCTTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCCCGGAGAGCCCCCGGCCC AGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCCGGGGCCACCCCCAGGCCCCCTGAGTAAAGCCCCCC AGCCTGTGCCGCCCGGGGTTGGGGAGCTGCCTGCCCGAGGCCCTCGACTCTTTGATTTTCCCCCCACTCC GCTGGAGGACCAGTTTGAGGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCCAACATCATGAAG ATGCTGGACGAATCCATTCGCAAGGAAGAGGAACAGCAACAACACGAAGCAGGCGTGGCCCCCCAACCCC CGCTGAAGGAGCCCTTTGCATCTCTGCAGTCTCCTTTCCCCACCGACACAGCCCCCACCACTACTGCTCC TGCTGTCGCCGTCACCACCACCACCACCACCACCACCACCACCACGGCCACCCAGGAAGAGGAGAAGAAG CCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTCCCTCCACCCTCTCAGCCACAGCCACCAC CACCCCCACCCCCCAGCCCGGCCAGCCTGCTCAAATCCTTGGCCTCCGTGCTGGAGGGACAAAAGTACTG TTATCGGGGGACTGGAGCAGCTGTTTCCACCCGGCCTGGGCCCTTGCCCACCACTCAGTATTCCCCTGGC CCCCCATCAGGTGCTACCGCCCTGCCGCCCACCTCAGCGGCCCCTAGCGCCCAGGGCTCCCCACAGCCCT CTGCTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCCTGGGCCCGGGAGCGCAGGGCGGGCGAAGA GCCAGTCCCGGGCCCCATGACCCCCACCCAACCGCCCCCACCCCTATCTCTGCCCCCTGCTCGCTCTGAG TCTGAGGTGCTAGAAGAGATCAGCCGGGCTTGCGAGACCCTTGTGGAGCGGGTGGGCCGGAGTGCCACTG ACCCAGCCGACCCAGTGGACACAGCAGAGCCAGCGGACAGTGGGACTGAGCGACTGCTGCCCCCCGCACA GGCCAAGGAGGAGGCTGGCGGGGTGGCGGCAGTGTCAGGCAGCTGTAAGCGGCGACAGAAGGAGCATCAG AAGGAGCATCGGCGGCACAGGCGGGCCTGTAAGGACAGTGTGGGTCGTCGGCCCCGTGAGGGCAGGGCAA AGGCCAAGGCCAAGGTCCCCAAAGAAAAGAGCCGCCGGGTGCTGGGGAACCTGGACCTGCAGAGCGAGGA GATCCAGGGTCGTGAGAAGTCCCGGCCCGATCTTGGCGGGGCCTCCAAGGCCAAGCCACCCACAGCTCCA GCCCCTCCATCAGCTCCTGCACCTTCTGCCCAGCCCACACCCCCGTCAGCCTCTGTCCCTGGAAAGAAGG CTCGGGAGGAAGCCCCAGGGCCACCGGGTGTCAGCCGGGCCGACATGCTGAAGCTGCGCTCACTTAGTGA GGGGCCCCCCAAGGAGCTGAAGATCCGGCTCATCAAGGTAGAGAGTGGTGACAAGGAGACCTTTATCGCC TCTGAGGTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCTGCTGACGTCGTGC GCGCCAGCAGGAATGCCAAGGTGAAAGGGAAGTTTCGAGAGTCCTACCTTTCCCCTGCCCAGTCTGTGAA ACCGAAGATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACACCCAGCATCTATCTG GAGAGCAAACGGGATGCCTTCTCACCTGTCCTGCTGCAGTTCTGTACAGACCCTCGAAATCCCATCACAG TGATCCGGGGCCTGGCGGGCTCCCTGCGGCTCAACTTGGGCCTCTTCTCCACCAAGACCCTGGTGGAAGC GAGTGGCGAACACACCGTGGAAGTTCGCACCCAGGTGCAGCAGCCCTCAGATGAGAACTGGGATCTGACA GGCACTCGGCAGATCTGGCCTTGTGAGAGCTCCCGTTCCCACACCACCATTGCCAAGTACGCACAGTACC AGGCCTCATCCTTCCAGGAGTCTCTGCAGGAGGAGAAGGAGAGTGAGGATGAGGAGTCAGAGGAGCCAGA CAGCACCACTGGAACCCCTCCTAGCAGCGCACCAGACCCGAAGAACCATCACATCATCAAGTTTGGCACC AACATCGACTTGTCTGATGCTAAGCGGTGGAAGCCCCAGCTGCAGGAGCTGCTGAAGCTGCCCGCCTTCA TGCGGGTAACATCCACGGGCAACATGCTGAGCCACGTGGGCCACACCATCCTGGGCATGAACACGGTGCA GCTGTACATGAAGGTGCCCGGCAGCCGAACGCCAGGCCACCAGGAGAATAACAACTTCTGCTCCGTCAAC ATCAACATTGGCCCAGGCGACTGCGAGTGGTTCGCGGTGCACGAGCACTACTGGGAGACCATCAGCGCTT TCTGTGATCGGCACGGCGTGGACTACTTGACGGGTTCCTGGTGGCCAATCCTGGATGATCTCTATGCATC CAATATTCCTGTGTACCGCTTCGTGCAGCGACCCGGAGACCTCGTGTGGATTAATGCGGGGACTGTGCAC TGGGTGCAGGCCACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACC AGCTGGCCCTGGAACGATACGAGTGGAATGAGGTGAAGAACGTCAAATCCATCGTGCCCATGATTCACGT GTCATGGAACGTGGCTCGCACGGTCAAAATCAGCGACCCCGACTTGTTCAAGATGATCAAGTTCTGCCTG CTGCAGTCCATGAAGCACTGCCAGGTGCAACGCGAGAGCCTGGTGCGGGCAGGGAAGAAAATCGCTTACC AGGGCCGTGTCAAGGACGAGCCAGCCTACTACTGCAACGAGTGCGATGTGGAGGTGTTTAACATCCTGTT CGTGACAAGTGAGAATGGCAGCCGCAACACGTACCTGGTACACTGCGAGGGCTGTGCCCGGCGCCGCAGC GCAGGCCTGCAGGGCGTGGTGGTGCTGGAGCAGTACCGCACTGAGGAGCTGGCTCAGGCCTACGACGCCT TCACGCTGGTGAGGGCCCGGCGGGCGCGCGGGCAGCGGAGGAGGGCACTGGGGCAGGCTGCAGGGACGGG CTTCGGGAGCCCGGCCGCGCCTTTCCCTGAGCCCCCGCCGGCTTTCTCCCCCCAGGCCCCAGCCAGCACG TCGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219461 representing NM_001080424
Red=Cloning site Green=Tags(s) MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLPAPLPPSHGSSSGH PSKPYYAPGAPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEATRCYHSALRYGGSF AELGPRIGRLQQAQLWNFHTGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVP PAALSGPSGEEGLSPGGKRRRGCNSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPLPGLATSPPFQLTKPG LWSTLHGDAWGPERKGSAPPERQEQRHSLPHPYPYPAPAYTAHPPGHRLVPAAPPGPGPRPPGAESHGCL PATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSNTGLRGVEPNPGIP GADHYQTPALEVSHHGRLGPSAHSSRKPFLGAPAATPHLSLPPGPSSPPPPPCPRLLRPPPPPAWLKGPA CRAAREDGEILEELFFGTEGPPRPAPPPLPHREGFLGPPASRFSVGTQDSHTPPTPPTPTTSSSNSNSGS HSSSPAGPVSFPPPPYLARSIDPLPRPPSPAQNPQDPPLVPLTLALPPAPPSSCHQNTSGSFRRPESPRP RVSFPKTPEVGPGPPPGPLSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMK MLDESIRKEEEQQQHEAGVAPQPPLKEPFASLQSPFPTDTAPTTTAPAVAVTTTTTTTTTTTATQEEEKK PPPALPPPPPLAKFPPPSQPQPPPPPPPSPASLLKSLASVLEGQKYCYRGTGAAVSTRPGPLPTTQYSPG PPSGATALPPTSAAPSAQGSPQPSASSSSQFSTSGGPWARERRAGEEPVPGPMTPTQPPPPLSLPPARSE SEVLEEISRACETLVERVGRSATDPADPVDTAEPADSGTERLLPPAQAKEEAGGVAAVSGSCKRRQKEHQ KEHRRHRRACKDSVGRRPREGRAKAKAKVPKEKSRRVLGNLDLQSEEIQGREKSRPDLGGASKAKPPTAP APPSAPAPSAQPTPPSASVPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIA SEVEERRLRMADLTISHCAADVVRASRNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYL ESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLT GTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGT NIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVN INIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVH WVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCL LQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRS AGLQGVVVLEQYRTEELAQAYDAFTLVRARRARGQRRRALGQAAGTGFGSPAAPFPEPPPAFSPQAPAST SR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080424 |
| ORF Size | 5046 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080424.2 |
| RefSeq Size | 6704 bp |
| RefSeq ORF | 5049 bp |
| Locus ID | 23135 |
| UniProt ID | O15054 |
| MW | 180.5 kDa |
| Gene Summary | The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways
,Johnstone, AL;Andrade, NS;Barbier, E;Khomtchouk, BB;Rienas, CA;Lowe, K;Van Booven, DJ;Domi, E;Esanov, R;Vilca, S;Tapocik, JD;Rodriguez, K;Maryanski, D;Keogh, MC;Meinhardt, MW;Sommer, WH;Heilig, M;Zeier, Z;Wahlestedt, C;,
Addict Biol
,PubMed ID 31373129
[KDM6B]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219461L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 6B (KDM6B), Myc-DDK-tagged |
CNY 19,360.00 |
|
| RC219461L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 6B (KDM6B), Myc-DDK-tagged |
CNY 19,360.00 |
|
| RG219461 | KDM6B (tGFP-tagged) - Human lysine (K)-specific demethylase 6B (KDM6B) |
CNY 18,530.00 |
|
| SC315893 | KDM6B (untagged)-Human lysine (K)-specific demethylase 6B (KDM6B) |
CNY 18,096.00 |
