ODAM (NM_017855) Human Tagged ORF Clone

CAT#: RC218743

ODAM (Myc-DDK-tagged)-Human odontogenic, ameloblast asssociated (ODAM)

ORF Plasmid: DDK tGFP



  "NM_017855" in other vectors (7)

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CNY 3,600.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms APIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218743 representing NM_017855
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAATTATAATTCTTCTTGGATTCCTGGGAGCCACATTGTCAGCCCCACTTATCCCACAGCGTCTCA
TGTCTGCCAGCAATAGCAATGAGTTACTTCTTAATCTTAATAATGGTCAACTTTTGCCACTACAACTTCA
GGGCCCACTTAATTCATGGATTCCACCTTTCTCTGGAATTTTACAACAGCAGCAGCAGGCTCAAATTCCA
GGACTCTCCCAGTTCTCTTTATCAGCTCTAGACCAGTTTGCTGGACTGCTCCCAAATCAGATACCCTTAA
CAGGAGAGGCCAGTTTTGCCCAAGGAGCCCAGGCAGGCCAAGTTGATCCCTTACAGCTTCAAACACCGCC
TCAGACACAACCAGGCCCCAGTCACGTGATGCCCTATGTATTCTCCTTCAAAATGCCTCAAGAGCAAGGA
CAGATGTTTCAATACTATCCAGTTTACATGGTCCTACCCTGGGAACAACCTCAGCAAACAGTTCCAAGGT
CACCTCAACAAACAAGACAGCAACAGTATGAGGAGCAGATACCATTCTATGCTCAATTTGGATACATTCC
ACAACTAGCAGAACCTGCTATATCAGGAGGACAGCAGCAACTAGCTTTTGATCCCCAACTAGGCACAGCT
CCTGAAATTGCTGTGATGTCAACAGGAGAAGAGATACCATATTTACAAAAAGAAGCGATCAACTTTAGAC
ATGACAGTGCAGGAGTTTTCATGCCCTCAACTTCACCAAAACCCAGCACAACCAATGTTTTCACTTCTGC
TGTAGACCAAACTATTACCCCAGAGCTCCCAGAAGAGAAGGACAAGACTGACAGCCTAAGGGAACCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218743 representing NM_017855
Red=Cloning site Green=Tags(s)

MKIIILLGFLGATLSAPLIPQRLMSASNSNELLLNLNNGQLLPLQLQGPLNSWIPPFSGILQQQQQAQIP
GLSQFSLSALDQFAGLLPNQIPLTGEASFAQGAQAGQVDPLQLQTPPQTQPGPSHVMPYVFSFKMPQEQG
QMFQYYPVYMVLPWEQPQQTVPRSPQQTRQQQYEEQIPFYAQFGYIPQLAEPAISGGQQQLAFDPQLGTA
PEIAVMSTGEEIPYLQKEAINFRHDSAGVFMPSTSPKPSTTNVFTSAVDQTITPELPEEKDKTDSLREP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017855
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017855.3, NP_060325.3
RefSeq Size 1301 bp
RefSeq ORF 840 bp
Locus ID 54959
UniProt ID A1E959
MW 30.6 kDa
Gene Summary Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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