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GPR111 (ADGRF2) (NM_153839) Human Tagged ORF Clone
CAT＃: RC218674
- TrueORF®
GPR111 (Myc-DDK-tagged)-Human G protein-coupled receptor 111 (GPR111)
ORF Plasmid: tGFP
"NM_153839" in other vectors (4)
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CNY 7,168.00
CNY 7,505.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR111; hGPCR35; PGR20 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218674 representing NM_153839
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCATATACTTTTGCTGTACTACTTGGTGTTTCTTTTGCCCACAGAGTCCTGTAGGACATTGTATC AGGCTGCAAGCAAAAGCAAGGAGAAGGTGCCTGCCAGGCCACACGGTGTATGCGATGGTGTCTGTACAGA CTACTCCCAGTGTACTCAACCTTGCCCTCCAGACACTCAGGGAAATATGGGGTTTTCATGCAGGCAAAAG ACATGGCACAAGATCACTGACACCTGCCAGACTCTTAATGCCCTCAACATCTTTGAGGAGGATTCACGTT TGGTTCAGCCATTTGAAGACAATATAAAAATAAGTGTATATACTGGAAAGTCTGAGACCATAACAGATAT GTTGCTACAAAAGTGTCCCACAGATCTGTCTTGTGTAATTAGAAACATTCAGCAGTCTCCCTGGATACCA GGAAACATTGCCGTAATTGTGCAGCTCTTACACAACATATCAACAGCAATATGGACAGGTGTTGATGAGG CAAAGATGCAGAGTTACAGCACCATAGCCAACCACATTCTTAACAGCAAAAGCATCTCCAACTGGACTTT CATTCCTGACAGAAACAGCAGCTATATCCTGCTACATTCAGTCAACTCCTTTGCAAGAAGGCTATTCATA GATAAACATCCTGTTGACATATCAGATGTCTTCATTCATACTATGGGCACCACCATATCTGGAGATAACA TTGGAAAAAATTTCACTTTTTCTATGAGAATTAATGATACCAGCAATGAAGTCACTGGGAGAGTGTTGAT CAGCAGAGATGAACTTCGGAAGGTGCCTTCCCCTTCTCAGGTCATCAGCATTGCATTTCCAACTATTGGG GCTATTTTGGAAGCCAGTCTTTTGGAAAATGTTACTGTAAATGGGCTTGTCCTGTCTGCCATTTTGCCCA AGGAACTTAAAAGAATCTCACTGATTTTTGAAAAGATCAGCAAGTCAGAGGAGAGGAGGACACAGTGTGT TGGCTGGCACTCTGTGGAGAACAGATGGGACCAGCAGGCCTGCAAAATGATTCAAGAAAACTCCCAGCAA GCTGTTTGCAAATGTAGGCCAAGCAAATTGTTTACCTCTTTCTCAATTCTTATGTCACCTCACATCTTAG AGAGTCTGATTCTGACTTACATCACATATGTAGGCCTGGGCATTTCTATTTGCAGCCTGATCCTTTGCTT GTCCATTGAGGTCCTAGTCTGGAGCCAAGTGACAAAGACAGAGATCACCTATTTACGCCATGTGTGCATT GTTAACATTGCAGCCACTTTGCTGATGGCAGATGTGTGGTTCATTGTGGCTTCCTTTCTTAGTGGCCCAA TAACACACCACAAGGGATGTGTGGCAGCCACATTTTTTGTTCATTTCTTTTACCTTTCTGTATTTTTCTG GATGCTTGCCAAGGCACTCCTTATCCTCTATGGAATCATGATTGTTTTCCATACCTTGCCCAAGTCAGTC CTGGTGGCATCTCTGTTTTCAGTGGGCTATGGATGCCCTTTGGCCATTGCTGCCATCACTGTTGCTGCCA CTGAACCTGGCAAAGGCTATCTACGACCTGAGATCTGCTGGCTCAACTGGGACATGACCAAAGCCCTCCT GGCCTTCGTGATCCCAGCTTTGGCCATCGTGGTAGTAAACCTGATCACAGTCACACTGGTGATTGTCAAG ACCCAGCGAGCTGCCATTGGCAATTCCATGTTCCAGGAAGTGAGAGCCATTGTGAGAATCAGCAAGAACA TCGCCATCCTCACACCACTTCTGGGACTGACCTGGGGATTTGGAGTAGCCACTGTCATCGATGACAGATC CCTGGCCTTCCACATTATCTTCTCCCTGCTCAATGCATTCCAGGGTTTCTTCATCCTAGTGTTTGGAACC ATCCTGGATCCAAAGATAAGAGAAGCCTTAAAGGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218674 representing NM_153839
Red=Cloning site Green=Tags(s) MTHILLLYYLVFLLPTESCRTLYQAASKSKEKVPARPHGVCDGVCTDYSQCTQPCPPDTQGNMGFSCRQK TWHKITDTCQTLNALNIFEEDSRLVQPFEDNIKISVYTGKSETITDMLLQKCPTDLSCVIRNIQQSPWIP GNIAVIVQLLHNISTAIWTGVDEAKMQSYSTIANHILNSKSISNWTFIPDRNSSYILLHSVNSFARRLFI DKHPVDISDVFIHTMGTTISGDNIGKNFTFSMRINDTSNEVTGRVLISRDELRKVPSPSQVISIAFPTIG AILEASLLENVTVNGLVLSAILPKELKRISLIFEKISKSEERRTQCVGWHSVENRWDQQACKMIQENSQQ AVCKCRPSKLFTSFSILMSPHILESLILTYITYVGLGISICSLILCLSIEVLVWSQVTKTEITYLRHVCI VNIAATLLMADVWFIVASFLSGPITHHKGCVAATFFVHFFYLSVFFWMLAKALLILYGIMIVFHTLPKSV LVASLFSVGYGCPLAIAAITVAATEPGKGYLRPEICWLNWDMTKALLAFVIPALAIVVVNLITVTLVIVK TQRAAIGNSMFQEVRAIVRISKNIAILTPLLGLTWGFGVATVIDDRSLAFHIIFSLLNAFQGFFILVFGT ILDPKIREALKG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153839 |
| ORF Size | 1926 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153839.7 |
| RefSeq Size | 5135 bp |
| RefSeq ORF | 1929 bp |
| Locus ID | 222611 |
| UniProt ID | Q8IZF7 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 71.6 kDa |
| Gene Summary | Orphan receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218674L3 | Lenti-ORF clone of GPR111 (Myc-DDK-tagged)-Human G protein-coupled receptor 111 (GPR111) |
CNY 5,890.00 |
|
| RC218674L4 | Lenti-ORF clone of GPR111 (mGFP-tagged)-Human G protein-coupled receptor 111 (GPR111) |
CNY 5,890.00 |
|
| RG218674 | GPR111 (tGFP-tagged) - Human G protein-coupled receptor 111 (GPR111) |
CNY 4,370.00 |
|
| SC306669 | GPR111 (untagged)-Human G protein-coupled receptor 111 (GPR111) |
CNY 7,176.00 |
