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Gemin 5 (GEMIN5) (NM_015465) Human Tagged ORF Clone
CAT＃: RC217516
GEMIN5 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 5 (GEMIN5)
ORF Plasmid: tGFP
"NM_015465" in other vectors (4)
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CNY 10,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GEMIN-5; NEDCAM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217516 representing NM_015465
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGGAGCCGCGGACGCTGCCGCCCTCCCCCAACTGGTACTGCGCCCGCTGCAGCGATGCCGTGC CCGGGGGCCTCTTTGGCTTCGCCGCGCGGACCTCCGTCTTCCTTGTCCGCGTGGGCCCGGGCGCAGGCGA GAGTCCAGGGACACCCCCGTTTCGAGTCATAGGAGAGTTGGTGGGACACACCGAAAGGGTCTCTGGCTTC ACATTTTCTCATCACCCTGGTCAGTACAACCTCTGTGCCACCAGCTCCGACGATGGGACTGTGAAAATAT GGGATGTAGAGACAAAAACAGTTGTGACAGAACATGCACTCCATCAGCATACGATATCAACATTACATTG GTCTCCTCGAGTAAAGGACTTAATAGTATCTGGGGATGAAAAAGGAGTAGTTTTCTGTTACTGGTTTAAC AGAAATGACAGCCAGCACCTCTTTATAGAACCCAGGACAATTTTCTGTCTTACTTGTTCACCTCATCATG AAGATTTAGTAGCCATTGGCTACAAGGATGGCATAGTGGTGATAATTGACATCAGTAAGAAAGGAGAAGT TATTCATAGGCTTCGAGGCCATGATGATGAAATCCACTCCATAGCCTGGTGTCCCCTGCCTGGTGAAGAT TGTTTATCTATAAACCAAGAGGAAACTTCAGAAGAAGCTGAAATTACCAACGGGAATGCTGTAGCACAAG CTCCAGTAACAAAAGGTTGCTACTTAGCCACTGGAAGCAAAGATCAAACCATTCGAATCTGGAGCTGTTC TAGAGGCCGAGGGGTGATGATTTTGAAATTGCCCTTTCTGAAGAGAAGAGGAGGGGGTATAGACCCAACT GTTAAAGAGCGCCTTTGGTTGACACTCCATTGGCCCAGCAATCAACCAACACAGCTGGTATCTAGCTGTT TTGGAGGTGAACTGTTGCAATGGGATCTCACTCAATCTTGGAGACGGAAATACACCCTCTTCAGTGCCTC ATCAGAAGGGCAAAATCATTCAAGAATTGTGTTTAATTTATGTCCTTTACAAACAGAGGATGACAAACAG CTATTACTTTCTACATCAATGGATAGAGATGTAAAATGTTGGGACATAGCCACCTTGGAGTGCAGCTGGA CCCTTCCTTCCCTTGGTGGGTTTGCATACAGCCTGGCTTTCTCTTCTGTGGACATAGGCTCTTTGGCCAT AGGTGTTGGGGATGGCATGATCCGTGTATGGAATACACTCTCCATAAAGAACAACTATGATGTGAAAAAT TTTTGGCAAGGCGTGAAGTCCAAGGTTACAGCGCTGTGCTGGCACCCAACCAAGGAAGGTTGCTTAGCTT TTGGAACTGATGATGGAAAAGTGGGATTGTATGACACCTACTCCAACAAGCCTCCACAGATTTCTAGCAC ATATCATAAGAAGACTGTATATACTTTAGCCTGGGGGCCACCAGTACCCCCCATGTCACTTGGAGGAGAA GGAGACAGACCTTCCCTTGCTTTATACAGCTGTGGAGGAGAAGGGATTGTCTTACAGCATAATCCCTGGA AGCTTAGTGGAGAAGCCTTTGACATCAACAAACTCATCAGGGACACCAATTCAATCAAATACAAATTGCC TGTACACACAGAGATAAGTTGGAAAGCAGATGGCAAAATCATGGCTCTTGGCAATGAAGATGGATCAATA GAAATATTTCAGATTCCCAACCTGAAACTGATCTGTACTATCCAACAGCATCACAAGCTTGTGAATACCA TTAGCTGGCATCATGAGCATGGCAGCCAGCCAGAATTGAGCTATCTGATGGCCTCTGGCTCCAACAATGC AGTCATTTACGTGCACAACCTGAAGACTGTCATAGAGAGCAGCCCTGAGTCTCCAGTGACCATTACAGAG CCCTACCGGACCCTCTCAGGGCATACGGCCAAGATTACCAGTGTGGCGTGGAGCCCACATCATGATGGAA GGCTGGTATCTGCTTCCTATGATGGTACAGCCCAGGTGTGGGATGCTCTCCGGGAAGAGCCCCTGTGCAA TTTCCGAGGACATCGAGGTCGACTGCTTTGTGTGGCATGGTCTCCTTTGGATCCAGACTGCATCTATTCA GGGGCAGATGACTTTTGTGTGCACAAGTGGCTCACTTCCATGCAAGATCATTCCCGGCCTCCTCAAGGCA AAAAAAGTATTGAATTGGAGAAAAAACGGCTCTCTCAACCTAAGGCAAAGCCCAAAAAGAAGAAAAAGCC CACCTTGAGAACTCCTGTAAAGCTGGAATCGATTGATGGAAATGAAGAAGAAAGCATGAAGGAGAACTCA GGACCTGTTGAGAATGGTGTGTCAGACCAAGAAGGGGAGGAGCAAGCACGGGAGCCGGAATTACCCTGTG GCCTTGCTCCAGCGGTTTCTAGAGAACCAGTTATCTGCACTCCAGTTTCCTCAGGCTTTGAAAAGTCAAA AGTCACCATTAATAACAAAGTCATTTTACTGAAAAAGGAGCCACCAAAAGAGAAGCCAGAAACCTTAATC AAGAAGAGAAAAGCTCGTTCCTTGCTTCCCCTGAGTACAAGCCTGGACCACAGATCCAAAGAGGAGCTTC ATCAGGACTGTTTGGTACTAGCAACTGCAAAGCACTCCAGAGAGCTGAATGAAGATGTGTCTGCTGATGT TGAGGAAAGATTTCATCTGGGGCTTTTCACAGACAGGGCTACCCTGTATAGAATGATTGATATTGAAGGA AAAGGTCACTTAGAAAATGGCCACCCTGAGTTATTTCACCAGCTTATGCTTTGGAAAGGAGATCTCAAAG GTGTTCTCCAGACTGCAGCAGAAAGAGGGGAGCTGACAGACAACCTTGTGGCTATGGCACCAGCAGCTGG CTACCATGTGTGGCTATGGGCTGTGGAAGCTTTTGCCAAACAGCTGTGTTTTCAGGATCAGTATGTCAAG GCTGCTTCTCACCTACTTTCCATCCACAAAGTGTATGAAGCGGTGGAGCTGCTCAAGTCAAACCATTTTT ACAGGGAAGCTATTGCGATTGCCAAGGCCCGGCTGCGCCCGGAGGACCCAGTCCTGAAGGACTTGTACCT CAGCTGGGGAACCGTCCTAGAAAGAGATGGCCACTATGCTGTAGCTGCCAAATGCTATTTAGGGGCCACT TGTGCTTATGATGCAGCCAAAGTTTTGGCCAAAAAGGGGGATGCGGCATCACTTAGAACGGCTGCAGAGT TGGCTGCCATCGTAGGAGAGGATGAGTTGTCTGCTTCCCTGGCTCTCAGATGTGCCCAAGAGCTGCTTCT GGCCAACAACTGGGTGGGAGCCCAGGAAGCCCTGCAGCTGCATGAAAGTCTACAGGGTCAGAGATTGGTG TTTTGCCTTCTGGAGCTACTGTCCAGGCATCTGGAGGAAAAGCAGCTTTCAGAGGGCAAAAGCTCCTCCT CTTACCACACTTGGAACACGGGCACCGAAGGGCCTTTCGTGGAGAGGGTGACTGCAGTGTGGAAGAGCAT CTTCAGCCTTGACACCCCTGAGCAGTATCAGGAAGCCTTTCAGAAGCTGCAGAACATCAAGTACCCATCT GCTACAAATAACACACCTGCCAAACAGCTCCTGCTTCACATTTGCCATGACTTGACCCTGGCAGTGCTGA GCCAACAGATGGCCTCCTGGGACGAGGCTGTGCAGGCGCTCCTTCGGGCGGTGGTCCGGAGCTATGACTC AGGGAGCTTCACCATCATGCAGGAAGTGTACTCAGCCTTTCTCCCTGATGGCTGTGACCACCTAAGAGAC AAGTTGGGGGACCATCAATCCCCTGCCACACCAGCTTTCAAAAGTTTGGAGGCCTTTTTTCTTTATGGGC GTCTGTATGAATTCTGGTGGTCTCTCTCCAGACCTTGCCCAAATTCCAGTGTCTGGGTAAGGGCTGGTCA CAGAACACTCTCTGTTGAGCCAAGCCAGCAGTTAGACACTGCCAGCACTGAAGAAACGGACCCTGAAACT TCTCAGCCAGAGCCAAACAGGCCTTCAGAACTAGACTTGAGACTCACAGAAGAAGGTGAGCGAATGCTGA GTACTTTTAAGGAGCTCTTTTCAGAAAAGCATGCCAGTCTCCAAAACTCACAGAGAACTGTTGCTGAAGT CCAAGAGACCTTGGCAGAAATGATCCGACAACACCAAAAGAGTCAACTCTGTAAATCCACAGCAAATGGT CCTGATAAGAATGAACCGGAAGTAGAAGCAGAGCAGCCCCTCTGCAGTTCTCAGAGCCAGTGTAAAGAAG AAAAAAATGAGCCACTTTCTCTGCCTGAGTTAACCAAAAGGCTTACCGAGGCAAATCAGAGAATGGCAAA ATTTCCTGAGAGCATTAAGGCCTGGCCCTTCCCAGATGTGCTGGAGTGCTGCCTCGTCCTGCTTCTCATC AGGTCCCACTTTCCTGGCTGTCTGGCCCAGGAAATGCAGCAGCAGGCCCAAGAGCTCCTTCAGAAATACG GCAACACGAAAACTTACAGAAGACACTGCCAGACCTTCTGTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217516 representing NM_015465
Red=Cloning site Green=Tags(s) MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKIWDVETKTVVTEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFN RNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGED CLSINQEETSEEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGGIDPT VKERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHSRIVFNLCPLQTEDDKQ LLLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDIGSLAIGVGDGMIRVWNTLSIKNNYDVKN FWQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGE GDRPSLALYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSI EIFQIPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPESPVTITE PYRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHRGRLLCVAWSPLDPDCIYS GADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAKPKKKKKPTLRTPVKLESIDGNEEESMKENS GPVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLI KKRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEG KGHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVK AASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAAKCYLGAT CAYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLALRCAQELLLANNWVGAQEALQLHESLQGQRLV FCLLELLSRHLEEKQLSEGKSSSSYHTWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPS ATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRD KLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPET SQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANG PDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLI RSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015465 |
| ORF Size | 4524 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015465.3 |
| RefSeq Size | 4527 bp |
| RefSeq ORF | 4527 bp |
| Locus ID | 25929 |
| UniProt ID | Q8TEQ6 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| MW | 168.4 kDa |
| Gene Summary | This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217516L3 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 5 (GEMIN5), Myc-DDK-tagged |
CNY 16,910.00 |
|
| RC217516L4 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 5 (GEMIN5), mGFP tagged |
CNY 12,720.00 |
|
| RG217516 | GEMIN5 (tGFP-tagged) - Human gem (nuclear organelle) associated protein 5 (GEMIN5) |
CNY 16,530.00 |
|
| SC309129 | GEMIN5 (untagged)-Human gem (nuclear organelle) associated protein 5 (GEMIN5) |
CNY 10,450.00 |
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