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SH3GL2 (NM_003026) Human Tagged ORF Clone
CAT＃: RC215806
SH3GL2 (Myc-DDK-tagged)-Human SH3-domain GRB2-like 2 (SH3GL2)
ORF Plasmid: tGFP
"NM_003026" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CNSA2; EEN-B1; SH3D2A; SH3P4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215806 representing NM_003026
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGCCGGCCTCAAGAAGCAGTTCCATAAAGCCACTCAGAAAGTGAGTGAGAAGGTTGGAGGAG CTGAAGGAACCAAGCTAGATGATGACTTCAAAGAGATGGAAAGGAAAGTGGATGTCACCAGCAGGGCTGT GATGGAAATAATGACTAAAACAATTGAATACCTTCAACCCAATCCAGCTTCCAGAGCTAAGCTCAGCATG ATCAACACCATGTCAAAAATCCGTGGCCAGGAGAAGGGGCCAGGCTATCCTCAGGCAGAGGCGCTGCTGG CAGAGGCCATGCTCAAATTTGGAAGAGAGCTTGGAGATGATTGCAACTTTGGCCCAGCACTTGGTGAGGT CGGGGAGGCCATGCGGGAACTGTCGGAGGTCAAAGACTCTTTGGACATAGAAGTGAAGCAGAACTTCATT GACCCTCTTCAGAATCTTCATGACAAAGATCTTAGGGAAATTCAACATCATCTAAAGAAGTTGGAGGGTC GACGCCTGGATTTTGATTATAAGAAGAAACGACAAGGCAAGATTCCGGATGAAGAGCTTCGTCAAGCTCT AGAGAAATTTGATGAGTCTAAGGAAATTGCTGAGTCAAGCATGTTCAATCTCTTGGAGATGGATATTGAA CAAGTGAGCCAGCTCTCTGCACTTGTGCAAGCTCAGCTGGAGTACCACAAGCAGGCAGTCCAGATCCTGC AGCAAGTCACGGTCAGACTGGAAGAAAGAATAAGACAGGCTTCATCTCAGCCTAGAAGGGAATATCAACC TAAACCACGAATGAGCCTGGAGTTTCCAACTGGAGACAGTACTCAGCCCAATGGGGGTCTCTCCCACACA GGCACTCCCAAACCTTCAGGTGTCCAAATGGATCAGCCCTGCTGCCGAGCTCTGTACGACTTTGAACCTG AAAATGAAGGGGAGTTGGGATTTAAAGAGGGCGATATCATCACACTCACTAACCAAATTGATGAGAACTG GTATGAGGGGATGCTGCATGGCCATTCAGGCTTCTTCCCCATCAATTATGTGGAAATTCTGGTTGCCCTG CCCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215806 representing NM_003026
Red=Cloning site Green=Tags(s) MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSM INTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFI DPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIE QVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSHT GTPKPSGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLHGHSGFFPINYVEILVAL PH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003026 |
| ORF Size | 1056 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003026.5 |
| RefSeq Size | 2540 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 6456 |
| UniProt ID | Q99962 |
| Domains | SH3, BAR |
| Protein Pathways | Endocytosis |
| MW | 39.8 kDa |
| Gene Summary | Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215806L1 | Lenti ORF clone of Human SH3-domain GRB2-like 2 (SH3GL2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215806L2 | Lenti ORF clone of Human SH3-domain GRB2-like 2 (SH3GL2), mGFP tagged |
CNY 5,890.00 |
|
| RC215806L3 | Lenti ORF clone of Human SH3-domain GRB2-like 2 (SH3GL2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215806L4 | Lenti ORF clone of Human SH3-domain GRB2-like 2 (SH3GL2), mGFP tagged |
CNY 5,890.00 |
|
| RG215806 | SH3GL2 (tGFP-tagged) - Human SH3-domain GRB2-like 2 (SH3GL2) |
CNY 4,370.00 |
|
| SC118257 | SH3GL2 (untagged)-Human SH3-domain GRB2-like 2 (SH3GL2) |
CNY 3,656.00 |
