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ITIH5 (NM_030569) Human Tagged ORF Clone
CAT＃: RC214536
ITIH5 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1
ORF Plasmid: tGFP
"NM_030569" in other vectors (4)
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CNY 7,000.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ITI-HC5; PP14776 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214536 representing NM_030569
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTGCTGCTGGGGCTGTGCCTGGGGCTGTCCCTGTGTGTGGGGTCGCAGGAAGAGGCGCAGAGCT GGGGCCACTCTTCGGAGCAGGATGGACTCAGGGTCCCGAGGCAAGTCAGACTGTTGCAGAGGCTGAAAAC CAAACCTTTGATGACAGAATTCTCAGTGAAGTCTACCATCATTTCCCGTTATGCCTTCACTACGGTTTCC TGCAGAATGCTGAACAGAGCTTCTGAAGACCAGGACATTGAGTTCCAGATGCAGATTCCAGCTGCAGCTT TCATCACCAACTTCACTATGCTTATTGGAGACAAGGTGTATCAGGGCGAAATTACAGAGAGAGAAAAGAA GAGTGGTGATAGGGTAAAAGAGAAAAGGAATAAAACCACAGAAGAAAATGGAGAGAAGGGGACTGAAATA TTCAGAGCTTCTGCAGTGATTCCCAGCAAGGACAAAGCCGCCTTTTTCCTGAGTTATGAGGAGCTTCTGC AGAGGCGCCTGGGCAAGTACGAGCACAGCATCAGCGTGCGGCCCCAGCAGCTGTCCGGGAGGCTGAGCGT GGACGTGAATATCCTGGAGAGCGCGGGCATCGCATCCCTGGAGGTGCTGCCGCTTCACAACAGCAGGCAG AGGGGCAGTGGGCGCGGGGAAGATGATTCTGGGCCTCCCCCATCTACTGTCATTAACCAAAATGAAACAT TTGCCAACATAATTTTTAAACCTACTGTAGTACAACAAGCCAGGATTGCCCAGAATGGAATTTTGGGAGA CTTTATCATTAGATATGACGTCAATAGAGAACAGAGCATTGGGGACATCCAGGTTCTAAATGGCTATTTT GTGCACTACTTTGCTCCTAAAGACCTTCCTCCTTTACCCAAGAATGTGGTATTCGTGCTTGACAGCAGTG CTTCTATGGTGGGAACCAAACTCCGGCAGACCAAGGATGCCCTCTTCACAATTCTCCATGACCTCCGACC CCAGGACCGTTTCAGTATCATTGGATTTTCCAACCGGATCAAAGTATGGAAGGACCACTTGATATCAGTC ACTCCAGACAGCATCAGGGATGGGAAAGTGTACATTCACCATATGTCACCCACTGGAGGCACAGACATCA ACGGGGCCCTGCAGAGGGCCATCAGGCTCCTCAACAAGTACGTGGCCCACAGTGGCATTGGAGACCGGAG CGTGTCCCTCATCGTCTTCCTGACGGATGGGAAGCCCACGGTCGGGGAGACGCACACCCTCAAGATCCTC AACAACACCCGAGAGGCCGCCCGAGGCCAAGTCTGCATCTTCACCATTGGCATCGGCAACGACGTGGACT TCAGGCTGCTGGAGAAACTGTCGCTGGAGAACTGTGGCCTCACACGGCGCGTGCACGAGGAGGAGGACGC AGGCTCGCAGCTCATCGGGTTCTACGATGAAATCAGGACCCCGCTCCTCTCTGACATCCGCATCGATTAT CCCCCCAGCTCAGTGGTGCAGGCCACCAAGACCCTGTTCCCCAACTACTTCAACGGCTCGGAGATCATCA TTGCGGGGAAGCTGGTGGACAGGAAGCTGGATCACCTGCACGTGGAGGTCACCGCCAGCAACAGTAAGAA ATTCATCATCCTGAAGACAGATGTGCCTGTGCGGCCTCAGAAGGCAGGGAAAGATGTCACAGGAAGCCCC AGGCCTGGAGGCGATGGAGAGGGGGACACCAACCACATCGAGCGTCTCTGGAGCTACCTCACCACAAAGG AGCTGCTGAGCTCCTGGCTGCAAAGTGACGATGAACCGGAGAAGGAGCGGCTGCGGCAGCGGGCCCAGGC CCTGGCTGTGAGCTACCGCTTCCTCACTCCCTTCACCTCCATGAAGCTGAGGGGGCCGGTCCCACGCATG GATGGCCTGGAGGAGGCCCACGGCATGTCGGCTGCCATGGGACCCGAACCGGTGGTGCAGAGCGTGCGAG GAGCTGGCACGCAGCCAGGACCTTTGCTCAAGAAGCCATACCAGCCAAGAATTAAAATCTCTAAAACATC AGTGGATGGTGATCCCCACTTTGTTGTGGATTTCCCCCTGAGCAGACTCACCGTGTGCTTCAACATTGAT GGGCAGCCCGGGGACATCCTCAGGCTGGTCTCTGATCACAGGGACTCTGGTGTCACAGTGAACGGAGAGT TAATTGGGGCACCCGCCCCTCCAAATGGCCACAAGAAACAGCGCACTTACTTGCGCACTATCACCATCCT CATCAACAAGCCAGAGAGATCTTATCTCGAGATCACACCGAGCAGAGTCATCTTGGATGGTGGGGACAGA CTGGTGCTCCCCTGCAACCAGAGTGTGGTGGTGGGGAGCTGGGGGCTGGAGGTGTCCGTGTCTGCCAACG CCAATGTCACCGTCACCATCCAGGGCTCCATAGCCTTTGTCATCCTCATCCACCTCTACAAAAAGCCGGC GCCCTTCCAGCGACACCACCTGGGTTTCTACATTGCCAACAGCGAGGGCCTTTCCAGCAACTGCCACGGA CTGCTGGGTCAGTTCCTGAATCAGGATGCCAGACTCACAGAAGACCCTGCAGGGCCCAGCCAGAACCTCA CTCACCCTCTGCTCCTTCAGGTGGGAGAGGGGCCTGAGGCCGTCCTAACAGTGAAAGGCCACCAAGTCCC AGTGGTCTGGAAGCAAAGGAAGATTTACAACGGGGAAGAGCAGATAGACTGCTGGTTTGCCAGGAACAAT GCCGCCAAACTGATTGACGGGGAGTACAAGGATTACCTGGCATCCCATCCATTTGACACAGGGATGGACA CTTGGCCGGGGAATGTCCAGGGAGCTCTGAAGCTGGCAGCCTTAAAGATGCAAGTGCATGAAGGACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214536 representing NM_030569
Red=Cloning site Green=Tags(s) MLLLLGLCLGLSLCVGSQEEAQSWGHSSEQDGLRVPRQVRLLQRLKTKPLMTEFSVKSTIISRYAFTTVS CRMLNRASEDQDIEFQMQIPAAAFITNFTMLIGDKVYQGEITEREKKSGDRVKEKRNKTTEENGEKGTEI FRASAVIPSKDKAAFFLSYEELLQRRLGKYEHSISVRPQQLSGRLSVDVNILESAGIASLEVLPLHNSRQ RGSGRGEDDSGPPPSTVINQNETFANIIFKPTVVQQARIAQNGILGDFIIRYDVNREQSIGDIQVLNGYF VHYFAPKDLPPLPKNVVFVLDSSASMVGTKLRQTKDALFTILHDLRPQDRFSIIGFSNRIKVWKDHLISV TPDSIRDGKVYIHHMSPTGGTDINGALQRAIRLLNKYVAHSGIGDRSVSLIVFLTDGKPTVGETHTLKIL NNTREAARGQVCIFTIGIGNDVDFRLLEKLSLENCGLTRRVHEEEDAGSQLIGFYDEIRTPLLSDIRIDY PPSSVVQATKTLFPNYFNGSEIIIAGKLVDRKLDHLHVEVTASNSKKFIILKTDVPVRPQKAGKDVTGSP RPGGDGEGDTNHIERLWSYLTTKELLSSWLQSDDEPEKERLRQRAQALAVSYRFLTPFTSMKLRGPVPRM DGLEEAHGMSAAMGPEPVVQSVRGAGTQPGPLLKKPYQPRIKISKTSVDGDPHFVVDFPLSRLTVCFNID GQPGDILRLVSDHRDSGVTVNGELIGAPAPPNGHKKQRTYLRTITILINKPERSYLEITPSRVILDGGDR LVLPCNQSVVVGSWGLEVSVSANANVTVTIQGSIAFVILIHLYKKPAPFQRHHLGFYIANSEGLSSNCHG LLGQFLNQDARLTEDPAGPSQNLTHPLLLQVGEGPEAVLTVKGHQVPVVWKQRKIYNGEEQIDCWFARNN AAKLIDGEYKDYLASHPFDTGMDTWPGNVQGALKLAALKMQVHEGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030569 |
| ORF Size | 2868 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030569.3, NP_085046.3 |
| RefSeq Size | 6331 bp |
| RefSeq ORF | 2829 bp |
| Locus ID | 80760 |
| UniProt ID | Q86UX2 |
| Domains | VWA, VIT |
| MW | 105.9 kDa |
| Gene Summary | This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214536L3 | Lenti-ORF clone of ITIH5 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 |
CNY 9,030.00 |
|
| RC214536L4 | Lenti-ORF clone of ITIH5 (mGFP-tagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 |
CNY 9,030.00 |
|
| RG214536 | ITIH5 (tGFP-tagged) - Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 |
CNY 7,890.00 |
|
| SC315637 | ITIH5 (untagged)-Human inter-alpha (globulin) inhibitor H5 (ITIH5), transcript variant 1 |
CNY 18,430.00 |
