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NLN (NM_020726) Human Tagged ORF Clone
CAT＃: RC212447
NLN (Myc-DDK-tagged)-Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_020726" in other vectors (4)
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CNY 5,160.00
CNY 7,410.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGTBP; EP24.16; MEP; MOP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212447 representing NM_020726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCCGGTGCCTTTTGGCTGTGCGAAGCCTCCGCAGAGTTGGTGGTTCCAGGATTTTACTCAGAA TGACGTTAGGAAGAGAAGTGATGTCTCCTCTTCAGGCAATGTCTTCCTATACTGTGGCTGGCAGAAATGT TTTAAGATGGGATCTTTCACCAGAGCAAATTAAAACAAGAACTGAGGAGCTCATTGTGCAGACCAAACAG GTGTACGATGCTGTTGGAATGCTCGGTATTGAGGAAGTAACTTACGAGAACTGTCTGCAGGCACTGGCAG ATGTAGAAGTAAAGTATATAGTGGAAAGGACCATGCTAGACTTTCCCCAGCATGTATCCTCTGACAAAGA AGTACGAGCAGCAAGTACAGAAGCAGACAAAAGACTTTCTCGTTTTGATATTGAGATGAGCATGAGAGGA GATATATTTGAGAGAATTGTTCATTTACAGGAAACCTGTGATCTGGGGAAGATAAAACCTGAGGCCAGAC GATACTTGGAAAAGTCAATTAAAATGGGGAAAAGAAATGGGCTCCATCTTCCTGAACAAGTACAGAATGA AATCAAATCAATGAAGAAAAGAATGAGTGAGCTATGTATTGATTTTAACAAAAACCTCAATGAGGATGAT ACCTTCCTTGTATTTTCCAAGGCTGAACTTGGTGCTCTTCCTGATGATTTCATTGACAGTTTAGAAAAGA CAGATGATGACAAGTATAAAATTACCTTAAAATATCCACACTATTTCCCTGTCATGAAGAAATGTTGTAT CCCTGAAACCAGAAGAAGGATGGAAATGGCTTTTAATACAAGGTGCAAAGAGGAAAACACCATAATTTTG CAGCAGCTACTCCCACTGCGAACCAAGGTGGCCAAACTACTCGGTTATAGCACACATGCTGACTTCGTCC TTGAAATGAACACTGCAAAGAGCACAAGCCGCGTAACAGCCTTTCTAGATGATTTAAGCCAGAAGTTAAA ACCCTTGGGTGAAGCAGAACGAGAGTTTATTTTGAATTTGAAGAAAAAGGAATGCAAAGACAGGGGTTTT GAATATGATGGGAAAATCAATGCCTGGGATCTATATTACTACATGACTCAGACAGAGGAACTCAAGTATT CCATAGACCAAGAGTTCCTCAAGGAATACTTCCCAATTGAGGTGGTCACTGAAGGCTTGCTGAACACCTA CCAGGAGTTGTTGGGACTTTCATTTGAACAAATGACAGATGCTCATGTTTGGAACAAGAGTGTTACACTT TATACTGTGAAGGATAAAGCTACAGGAGAAGTATTGGGACAGTTCTATTTGGACCTCTATCCAAGGGAAG GAAAATACAATCATGCGGCCTGCTTCGGTCTCCAGCCTGGCTGCCTTCTGCCTGATGGAAGCCGGATGAT GGCAGTGGCTGCCCTCGTGGTGAACTTCTCACAGCCAGTGGCAGGTCGTCCCTCTCTCCTGAGACACGAC GAGGTGAGGACTTACTTTCATGAGTTTGGTCACGTGATGCATCAGATTTGTGCACAGACTGATTTTGCAC GATTTAGCGGAACAAATGTGGAAACTGACTTTGTAGAGGTGCCATCGCAAATGCTTGAAAATTGGGTGTG GGACGTCGATTCCCTCCGAAGATTGTCAAAACATTATAAAGATGGAAGCCCTATTGCAGACGATCTGCTT GAAAAACTTGTTGCTTCTAGGCTGGTCAACACAGGTCTTCTGACCCTGCGCCAGATTGTTTTGAGCAAAG TTGATCAGTCTCTTCATACCAACACATCGCTGGATGCTGCAAGTGAATATGCCAAATACTGCTCAGAAAT ATTAGGAGTTGCAGCTACTCCAGGCACAAATATGCCAGCTACCTTTGGACATTTGGCAGGGGGATACGAT GGCCAATATTATGGATATCTTTGGAGTGAAGTATTTTCCATGGATATGTTTTACAGCTGTTTTAAAAAAG AAGGGATAATGAATCCAGAGGTTGGAATGAAATACAGAAACCTAATCCTGAAACCTGGGGGATCTCTGGA CGGCATGGACATGCTCCACAATTTCTTGAAACGTGAGCCAAACCAAAAAGCGTTCCTAATGAGTAGAGGC CTGCATGCTCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212447 representing NM_020726
Red=Cloning site Green=Tags(s) MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQ VYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRG DIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDD TFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIIL QQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGF EYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTL YTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHD EVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLL EKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYD GQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRG LHAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020726 |
| ORF Size | 2112 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020726.5 |
| RefSeq Size | 2882 bp |
| RefSeq ORF | 2115 bp |
| Locus ID | 57486 |
| UniProt ID | Q9BYT8 |
| Domains | Peptidase_M3 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Renin-angiotensin system |
| MW | 80.5 kDa |
| Gene Summary | This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability
,null,
Science Translational Medicine
,PubMed ID 32269163
[NLN]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212447L3 | Lenti ORF clone of Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 9,310.00 |
|
| RC212447L4 | Lenti ORF clone of Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 9,310.00 |
|
| RG212447 | NLN (tGFP-tagged) - Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein |
CNY 6,760.00 |
|
| SC111375 | NLN (untagged)-Human neurolysin (metallopeptidase M3 family) (NLN), nuclear gene encoding mitochondrial protein |
CNY 5,168.00 |
