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PLOD2 (NM_000935) Human Tagged ORF Clone
CAT＃: RC212266
- TrueORF®
PLOD2 (Myc-DDK-tagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2
ORF Plasmid: tGFP
"NM_000935" in other vectors (4)
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CNY 7,320.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BRKS2; LH2; TLH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212266 representing NM_000935
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGGATGCACGGTGAAGCCTCAGCTGCTGCTCCTGGCGCTCGTCCTCCACCCCTGGAATCCCTGTC TGGGTGCGGACTCGGAGAAGCCCTCGAGCATCCCCACAGATAAATTATTAGTCATAACTGTAGCAACAAA AGAAAGTGATGGATTCCATCGATTTATGCAGTCAGCCAAATATTTCAATTATACTGTGAAGGTCCTTGGT CAAGGAGAAGAATGGAGAGGTGGTGATGGAATTAATAGTATTGGAGGGGGCCAGAAAGTGAGATTAATGA AAGAAGTCATGGAACACTATGCTGATCAAGATGATCTGGTTGTCATGTTTACTGAATGCTTTGATGTCAT ATTTGCTGGTGGTCCAGAAGAAGTTCTAAAAAAATTCCAAAAGGCAAACCACAAAGTGGTCTTTGCAGCA GATGGAATTTTGTGGCCAGATAAAAGACTAGCAGACAAGTATCCTGTTGTGCACATTGGGAAACGCTATC TGAATTCAGGAGGATTTATTGGCTATGCTCCATATGTCAACCGTATAGTTCAACAATGGAATCTCCAGGA TAATGATGATGATCAGCTCTTTTACACTAAAGTTTACATTGATCCACTGAAAAGGGAAGCTATTAACATC ACATTGGATCACAAATGCAAAATTTTCCAGACCTTAAATGGAGCTGTAGATGAAGTTGTTTTAAAATTTG AAAATGGCAAAGCCAGAGCTAAGAATACATTTTATGAAACATTACCAGTGGCAATTAATGGAAATGGACC CACCAAGATTCTCCTGAATTATTTTGGAAACTATGTACCCAATTCATGGACACAGGATAATGGCTGCACT CTTTGTGAATTCGATACAGTCGACTTGTCTGCAGTAGATGTCCATCCAAACGTATCAATAGGTGTTTTTA TTGAGCAACCAACCCCTTTTCTACCTCGGTTTCTGGACATATTGTTGACACTGGATTACCCAAAAGAAGC ACTTAAACTTTTTATTCATAACAAAGAAGTTTATCATGAAAAGGACATCAAGGTATTTTTTGATAAAGCT AAGCATGAAATCAAAACTATAAAAATAGTAGGACCAGAAGAAAATCTAAGTCAAGCGGAAGCCAGAAACA TGGGAATGGACTTTTGCCGTCAGGATGAAAAGTGTGATTATTACTTTAGTGTGGATGCAGATGTTGTTTT GACAAATCCAAGGACTTTAAAAATTTTGATTGAACAAAACAGAAAGATCATTGCTCCTCTTGTAACTCGT CATGGAAAGCTGTGGTCCAATTTCTGGGGAGCATTGAGTCCTGATGGATACTATGCACGATCTGAAGATT ATGTGGATATTGTTCAAGGGAATAGAGTAGGAGTATGGAATGTCCCATATATGGCTAATGTGTACTTAAT TAAAGGAAAGACACTCCGATCAGAGATGAATGAAAGGAACTATTTTGTTCGTGATAAACTGGATCCTGAT ATGGCTCTTTGCCGAAATGCTAGAGAAATGGGTGTATTTATGTACATTTCTAATAGACATGAATTTGGAA GGCTATTATCCACTGCTAATTACAATACTTCCCATTATAACAATGACCTCTGGCAGATTTTTGAAAATCC TGTGGACTGGAAGGAAAAGTATATAAACCGTGATTATTCAAAGATTTTCACTGAAAATATAGTTGAACAG CCCTGTCCAGATGTCTTTTGGTTCCCCATATTTTCTGAAAAAGCCTGTGATGAATTGGTAGAAGAAATGG AACATTACGGCAAATGGTCTGGGGGAAAACATCATGATAGCCGTATATCTGGTGGTTATGAAAATGTCCC AACTGATGATATCCACATGAAGCAAGTTGATCTGGAGAATGTATGGCTTCATTTTATCCGGGAGTTCATT GCACCAGTTACACTGAAGGTCTTTGCAGGCTATTATACGAAGGGATTTGCACTACTGAATTTTGTAGTAA AATACTCCCCTGAACGACAGCGTTCTCTTCGTCCTCATCATGATGCTTCTACATTTACCATAAACATTGC ACTTAATAACGTGGGAGAAGACTTTCAGGGAGGTGGTTGCAAATTTCTAAGGTACAATTGCTCTATTGAG TCACCACGAAAAGGCTGGAGCTTCATGCATCCTGGGAGACTCACACATTTGCATGAAGGACTTCCTGTTA AAAATGGAACAAGATACATTGCAGTGTCATTTATAGATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212266 representing NM_000935
Red=Cloning site Green=Tags(s) MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLG QGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAA DGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINI TLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCT LCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKA KHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTR HGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPD MALCRNAREMGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQ PCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFI APVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIE SPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000935 |
| ORF Size | 2211 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000935.2, NP_000926.2 |
| RefSeq Size | 4009 bp |
| RefSeq ORF | 2214 bp |
| Locus ID | 5352 |
| UniProt ID | O00469 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Pathways | Lysine degradation |
| MW | 84.69 kDa |
| Gene Summary | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2
,Gjaltema, RA;van der Stoel, MM;Boersema, M;Bank, RA;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 27298363
[PLOD2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212266L3 | Lenti-ORF clone of PLOD2 (Myc-DDK-tagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2 |
CNY 9,220.00 |
|
| RC212266L4 | Lenti-ORF clone of PLOD2 (mGFP-tagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2 |
CNY 9,220.00 |
|
| RG212266 | PLOD2 (tGFP-tagged) - Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2 |
CNY 8,080.00 |
|
| SC314581 | PLOD2 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2 |
CNY 5,408.00 |
