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MNK2 (MKNK2) (NM_017572) Human Tagged ORF Clone
CAT＃: RC210081
MKNK2 (Myc-DDK-tagged)-Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1
ORF Plasmid: tGFP
"NM_017572" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPRK7; MNK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210081 representing NM_017572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGAAGAAACCAGCCGAACTTCAGGGTTTCCACCGTTCGTTCAAGGGGCAGAACCCCTTCGAGC TGGCCTTCTCCCTAGACCAGCCCGACCACGGAGACTCTGACTTTGGCCTGCAGTGCTCAGCCCGCCCTGA CATGCCCGCCAGCCAGCCCATTGACATCCCGGACGCCAAGAAGAGGGGCAAGAAGAAGAAGCGCGGCCGG GCCACCGACAGCTTCTCGGGCAGGTTTGAAGACGTCTACCAGCTGCAGGAAGATGTGCTGGGGGAGGGCG CTCATGCCCGAGTGCAGACCTGCATCAACCTGATCACCAGCCAGGAGTACGCCGTCAAGATCATTGAGAA GCAGCCAGGCCACATTCGGAGCAGGGTTTTCAGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACACAGG AACGTCCTAGAGCTGATTGAGTTCTTCGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAGAAGATGCGGG GAGGCTCCATCCTGAGCCACATCCACAAGCGCCGGCACTTCAACGAGCTGGAGGCCAGCGTGGTGGTGCA GGACGTGGCCAGCGCCTTGGACTTTCTGCATAACAAAGGCATCGCCCACAGGGACCTAAAGCCGGAAAAC ATCCTCTGTGAGCACCCCAACCAGGTCTCCCCCGTGAAGATCTGTGACTTCGACCTGGGCAGCGGCATCA AACTCAACGGGGACTGCTCCCCTATCTCCACCCCGGAGCTGCTCACTCCGTGCGGCTCGGCGGAGTACAT GGCCCCGGAGGTAGTGGAGGCCTTCAGCGAGGAGGCTAGCATCTACGACAAGCGCTGCGACCTGTGGAGC CTGGGCGTCATCTTGTATATCCTACTCAGCGGCTACCCGCCCTTCGTGGGCCGCTGTGGCAGCGACTGCG GCTGGGACCGCGGCGAGGCCTGCCCTGCCTGCCAGAACATGCTGTTTGAGAGCATCCAGGAGGGCAAGTA CGAGTTCCCCGACAAGGACTGGGCCCACATCTCCTGCGCTGCCAAAGACCTCATCTCCAAGCTGCTGGTC CGTGACGCCAAGCAGAGGCTGAGTGCCGCCCAAGTCCTGCAGCACCCCTGGGTTCAGGGGTGCGCCCCGG AGAACACCTTGCCCACTCCCATGGTCCTGCAGAGGTGGGACAGTCACTTCCTCCTCCCTCCCCACCCCTG TCGCATCCACGTGCGACCTGGAGGACTGGTCAGAACCGTTACTGTGAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210081 representing NM_017572
Red=Cloning site Green=Tags(s) MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIPDAKKRGKKKKRGR ATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHR NVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPEN ILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWS LGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLV RDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017572 |
| ORF Size | 1242 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017572.4 |
| RefSeq Size | 1778 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 2872 |
| UniProt ID | Q9HBH9 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Insulin signaling pathway, MAPK signaling pathway |
| MW | 46.5 kDa |
| Gene Summary | This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210081L1 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210081L2 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC210081L3 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210081L4 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210081 | MKNK2 (tGFP-tagged) - Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1 |
CNY 7,088.00 |
|
| SC315549 | MKNK2 (untagged)-Human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1 |
CNY 7,220.00 |
