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ALPK1 (NM_025144) Human Tagged ORF Clone
CAT＃: RC209189
- TrueORF®
ALPK1 (Myc-DDK-tagged)-Human alpha-kinase 1 (ALPK1), transcript variant 1
ORF Plasmid: tGFP
"NM_025144" in other vectors (7)
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CNY 12,992.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430410J10Rik; LAK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209189 representing NM_025144.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTG GAAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCCAGCGAG TTAAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCCTTCGTGCCTGAAAAGTGGCAGTACAAA CAAGCCGTGGGCCCAGAGGACAAAACAAACCTGAAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTG GCGTCCCTGAGGGCCTCCATCCTCGCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGAC CGGTTCCTGTATGGGCTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAG CCAGCCACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTT TTAAAAGCAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACCTGGCTGTACAGA AATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGAGGGCAGATTCTGCAAAAGCTG GGGATGTGGTACGAAGCAGCAGAGTTAATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCG GATAAAAAGGGCCTCTCCACGTCGCTAGGTATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGAT TATGAAAAGTTTAAAAACAATCCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTG TCCGCTGCAGAAGCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCT GTGAATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTGAAAAAC TTACATCTGTGTGAAGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTT ACTGGAAAACAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGTCTCACCACAGTGCACAGAAGGCTC CATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAGCTCTGTAAGGAAGCAATGGGGAAGCTGTACAAT TTCAGCACTTCCTCCAGAAGTCAGGACAGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAG GTGAAGGAACATTTACAAGTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTC GAGTGCAGGTTGGATAAACTTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCA CAGCACCATACTTCGGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGAAAGAT GCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAAAACATAGATACTGTGAGTACTACT CAAGAAAAGCCACATTGTCAAAGAGACACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGG GAACTCAGAAGGGGAGGAAGGAGAAACTGGACCCATTCTGATGCATTTCGAGTCTCCTTGGATCAAGAT GTGGAGACTGAGACTGAGCCATCGGACTACAGCAATGGTGAGGGAGCTGTTTTCAACAAGTCTCTGAGT GGCAGCCAGACTTCCAGTGCTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTG AATTATCACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAGTGT TCCACTGCCTTGTCTGAGGAGCTAGAGAATGACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAG CTTCATGATCTCTCTCTTCAGGAACCCAACAATGACAATTTGGAGCCTTCTCAAAATCAGCCACAGCAA CAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCAGGCATTTTCTTGGCCCCTGGTGCAGGGCTT CTAGAAGGAGCTCCAGAAGGTATCCAGGAAGTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCC AGACCCTCATATCGTTCTGCTTCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATATGGGCACACATCCT TCAGTCCAAAAAGAAGAAGCCTTTGAAATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGAC AGGCAGGGGAAAGAGCAGGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTCCC TCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCACCCTTAGACTTTCACAGGGTC CTGCACAATTCTCTGGGAAACATTTCCATGCTGCCATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAA AATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCAGAGCAGGGCATCGACCCTGATGCCTCCACAGTG GATGAGGAGGGGCAACTGCTCGACAGCATGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTG TGCATTCTGAGACAGCCGCCTGGTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTC TTCCCTGTCCTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGC AGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAATCACCTGCATTTTCCAGTGGTTCTTCTGAG GGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCATTGCCGGGAAAGATGAGG AAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTTGAAAAGCTGTTGGCAGGAGTGAGGCAT GATTGGCTGTTTCAGAGACTAGAGAATACGGGGGTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGT GCTCTTTTGTTAAAATATTCAAAAAAATCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGG GACTACTTGACTGTGAAGAAAAAAGGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAA GAAATTCTGGGGAGGTATGTTGGGAAAGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGAT GTGGAGCGACAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATT CCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAGGACAAGACAATAAAGGGATGT ATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAATTGTCAAATAACACGAAAGTGGTGAAAACA GAATACAAAGCCACAGAATATGGCTTGGCCTATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGAT GTTGTGGTCGATTTACAAGGTTGGGTAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAG ATTCACTCCGTTGATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAAT AACCAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAGAAACCA TGCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC209189
Blue=ORF Red=Cloning site Green=Tag(s) MNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKWQYK QAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQ PATPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKL GMWYEAAELIWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLL SAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPV TGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQ VKEHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKD AKTGVCITALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQD VETETEPSDYSNGEGAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHLVDTQC STALSEELENDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAGL LEGAPEGIQEVRNMGPRNTSAHSRPSYRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKD RQGKEQGEEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQ NPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSMDVPCTNGHGSHRLCILRQPPGQRAETPNSSVSGNIL FPVLSEDCTTTEEGNQPGNMLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMR KEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLG DYLTVKKKGRQRNAFWVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNI PTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRD VVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSMEKP CT TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC209189 also available, TP309189 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025144 |
| ORF Size | 3732 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025144.4 |
| RefSeq Size | 5399 bp |
| RefSeq ORF | 3735 bp |
| Locus ID | 80216 |
| UniProt ID | Q96QP1 |
| Domains | MHCK_EF2_kinase |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 138.9 kDa |
| Gene Summary | This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209189L1 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, Myc-DDK-tagged |
CNY 15,392.00 |
|
| RC209189L2 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RC209189L3 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, Myc-DDK-tagged |
CNY 15,392.00 |
|
| RC209189L4 | Lenti ORF clone of Human alpha-kinase 1 (ALPK1), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG209189 | ALPK1 (tGFP-tagged) - Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 10,550.00 |
|
| SC125538 | ALPK1 (untagged)-Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 8,672.00 |
|
| SC323654 | ALPK1 (untagged)-Kinase deficient mutant (K1067M) of Human alpha-kinase 1 (ALPK1), transcript variant 1 |
CNY 8,672.00 |
