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H2AC19 (NM_001040874) Human Tagged ORF Clone
CAT＃: RC200688
HIST2H2AA4 (Myc-DDK-tagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4)
ORF Plasmid: tGFP
"NM_001040874" in other vectors (6)
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CNY 1,800.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | H2A/R; H2AC18; HIST2H2AA4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200688 representing NM_001040874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGTCGTGGCAAGCAAGGAGGCAAGGCCCGCGCCAAGGCCAAGTCGCGCTCGTCCCGCGCTGGCC TTCAGTTCCCGGTAGGGCGAGTGCATCGCTTGCTGCGCAAAGGCAACTACGCGGAGCGAGTGGGGGCCGG CGCGCCCGTCTACATGGCTGCGGTCCTCGAGTATCTGACCGCCGAGATCCTGGAGCTGGCGGGCAACGCG GCTCGGGACAACAAGAAGACGCGCATCATCCCTCGTCACCTCCAGCTGGCCATCCGCAACGACGAGGAAC TGAACAAGCTGCTGGGCAAAGTCACCATCGCCCAGGGCGGCGTCTTGCCTAACATCCAGGCCGTACTGCT CCCTAAGAAGACGGAGAGTCACCACAAGGCAAAGGGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200688 representing NM_001040874
Red=Cloning site Green=Tags(s) MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNA ARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040874 |
| ORF Size | 390 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040874.1, NP_001035807.1 |
| RefSeq Size | 534 bp |
| RefSeq ORF | 393 bp |
| Locus ID | 723790 |
| UniProt ID | Q6FI13 |
| Protein Pathways | Systemic lupus erythematosus |
| MW | 14.1 kDa |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Evolution of histone 2A for chromatin compaction in eukaryotes
,null,
eLife
,PubMed ID 24939988
[H2AC19]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200688L1 | Lenti-ORF clone of HIST2H2AA4 (Myc-DDK-tagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 4,200.00 |
|
| RC200688L2 | Lenti-ORF clone of HIST2H2AA4 (mGFP-tagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 5,890.00 |
|
| RC200688L3 | Lenti-ORF clone of HIST2H2AA4 (Myc-DDK-tagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 5,890.00 |
|
| RC200688L4 | Lenti-ORF clone of HIST2H2AA4 (mGFP-tagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 5,890.00 |
|
| RG200688 | HIST2H2AA4 (tGFP-tagged) - Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 4,370.00 |
|
| SC311199 | HIST2H2AA4 (untagged)-Human histone cluster 2, H2aa4 (HIST2H2AA4) |
CNY 1,800.00 |
