Wnk3 (NM_001271678) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Prkwnk3; Wnk3-ps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231872 representing NM_001271678 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTGAAA ACAGGGCTGCCCGGGCTGTTGCCCCTCTGACTGTAGAAGCGAGAATAAAGGAAAAATACAGTACCTTTTC TGCTTCCGGGGAAAACATCGAAAGAAAGAGGTTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA GTTGCCGAATCTTCCCGCAGAGATGAAAGAAAAGCTGCAACAAATATTTCAAGAGTAGATAAAGTTCCTA CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAATGTTCAAAGGCAACCTCAGAATCCTCAAA AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTTGCAACTTCC CCTAGTGGCAGGTTCCTGAAATTCGATATAGAGCTGGGAAGAGGAGCATTTAAAACGGTATATAAAGGAT TGGACACTGAGACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA GCAACGGTTCAAAGAAGAAGCAGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTCTATGAT TCATGGGAGTCCACTTTGAAAGGAAAGAAATGTATTGTGTTAGTGACTGAACTAATGACATCAGGAACCT TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATCTT AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCGT TTGCTAAGAGTGTCATTGGCACTCCTGAATTTATGGCTCCGGAGATGTATGAAGAACACTATGATGAATC TGTAGATGTTTATGCTTTTGGAATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC CAGAATGCAGCACAAATATACCGGAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCTATCAAGGA CTTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGAGTGACATTGATT AAAAAGATAAGGGAAAAAAAGCCTGCTGGATGCTTGGAGGAACGCAGGGATTCCCAGTGCAAGTATGTGA GGAATGTACTTCCTCAACAGCAGACTGCAACTTTACAACCTACTCCTGGTCCACACACTGCAGCTGAGTA TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGTTTCTACAGGGCAAACCACAGCAGCAGTCCTCC TCTGTTAGGGGTGACACTTCATCTGAGCCAACAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCATC CCACTGTAGCCTATTCTTCAAACCAGACAACTAGCTCTCAGGAGCAGCCAAAGCTGACTCAGTCCCCAGT TTTGCCTGTGGTTCAAGGTCAGTCCTCTGTTATGCCCATATATGCTGCTGGAGTTGGAGTTGTTTCACAG TCCCAAATCTCTCCCTTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG AACAACAAGCAACTCTTCAAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA AAACACCAATAACCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGAACAAACAACGAAGAGCCTCC TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGAGACAACA TGGTGGAGTGTCAGCTGGAAACACATAACAACAAGATGGTGACATTCAAGTTTGATGTTGATGGTGACGC ACCTGAGGATATCGCTGACTATATGGTTGAAGATAACTTTGTGCTGGAAAATGAGAAAGAAAAATTTGTC GAGGAATTGAGGGCTATCGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTCAGCTGTAGAAAAATCTA TTGGTGTTGACTCTGTTGCCTTGGAATCTAACAGTAACCAAACAGGATCATCTGAACAAGTACTGATAAA TTCTGCATCCACTCAAACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGT ATCAACCAAACAATAAAAAACCGTGAGGCACAGTCTCCTCCTTCCCTTCAGCCTTCCATGGCCATGGTGC CTGGGTTACATCCATTTCCTAGTTCAAGAAACACAAGTAATCAGGCAATATCACAGAACACAGTGTTCAC TATAGAGAATAATCCAGGCCATCGTGAACTTTTCACCTCCAAATTAGACCACAAGGATGTGGTTGATGGT AAGATTGGTGAACATGCTAGTATAGAAACTGAGCAGTCAAGTATTTCCTATCAAGTGGAAGATGACAGAC AGATCATGACACCAGCTACTGATAATTCCAATTATTCTGCTGCTTTGGTGTGTCCAGTTCCAGGTGAATG TGAGGCACTCACCAGCCAAGCAGGCATGTTCATGCCTACATATCCAAATCAGCAAGCTGCTGTTCTGGCT GATGTGCATATAGCCTATCCCGGTGAATCAGTTCCAATTGGTGGTAATGCTGCTCTTACATCAGTACTTG TATCTTCTGATCAAAAGCCTCAATCCTTGTCAGTACAGCAGCCAACTATAGATGCAGAGTTTATTTCCAA AGAAGGAGAAACCACAGTAAACACCGAAACAAGTTCTCCTAAGGCAGTCATTGCCACTCAGACTCCTGGC TTTGAACCAGCTGTGATTCTACCTGCTACTATCCTGGAATCAGATGGGGAACGACCTCCAAAAATGGAGT TTGCAGACAACCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTATCAGGAGCACAGCATTTC TAGCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTTTCTTCCTCTGCTGAAGAT ATACTATCCTATTCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTCAAGATAGCCCTGCACAAT CCCCAAATTTCCAACAAACAGGCTCTAAGATTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTCATATATC AGTGTTCAAAAAAGACCTGAATGTGATAACTTCTGTACCCAGCGAGTTATGTTTACATGAGATGTCCCCA GATGCTTCACTTCCAGGCGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGATGGAACCATTCATCTGC AGACAGGAGGTGGATATTTTGGCCTAAGCTTTACTTGTCCTAGTCTCAAAAATCCTATTAGCAGGAAATC CTGGACTCGAAAATTAAAAAGCTGGGCATACAGGCTACGGCAGTCAACCAGCTTTTTCAAGAGATCAAAA GTCCGTCAAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCATTCCTCTGACACGGGAGT TCTCATCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCA GGTGATTACTGTTCCTCAGCAGCAGCCAGTAAAAATGATGTCTTTTGGGAAAGATCACAGACCGCCATTC AACAAAACAACAGTCCAGTCTAGTGAGCAAGCACTAACCTTTGCTGAAGCTGCAGTGTCGCAGTTGATCG AAGTGGAGCCTGCCATGCCCACTCATAAAGCTTCAGTTTCTTCACGCAAGTTGCGAACTCTTTATGAAAC CTTTAAAGAAGATAAAGGAGATCCTGAACAAGGTGACATCGTATCTTACAGCACAGCTTGTGAGACCAGT GTCTCTTCAGTCGCTACAGAAAAGAATGTAACTTCAACCACAGAAGTTAGTGTGCAGTCTGGGTCTGAAC CGTTAGATAAAGAAAAAAATGAGTCAACTCCTGGGAAACAGACATGTACAAATGAATTTTCAGCCACTCT TGCTGGTAATAGAAAGTCCGTGACAAAAACTCGTCCAGAGGGTGATCAGTATTTACCACTCCGTGAAGAG CAAGCCTATGCTCAAACACAGAATTCACTCTTCTATTCTCCATCTTCCCCAATGAGCAGTGACAATGAAT CAGAAATTGAGGACGAGGACTTGAAAGTGGAGCTTCAAAGATTACGAGAAAAACACATTCAAGAGGTGGT CAGTCTTCAAACACAACAGAATAAAGAGCTACAAGAGCTCTATGAACGCCTTCGAGCAACTAAAGACAAC AAAGCGCAATCTTCAGAGGTTCCTTTGTCACCTGCATCGCCACGCAGGCCAAGATCTTTCAAAAGCAAAC TTCGAAGCCGCCCCCAGTCCATGACACATTCAGACAATCTCGTTGTAAAAGATGCACTGGGTGTGGAGAG TAATACCGTGTCATGCCAGCAATCTCCAGCCAGTAAGAAGGGGATGTTCACAGATGATTTACACAAGCTG GTGGATGACTGGACAAGAGAAACAGTAGGGCATTTTCCTAGTAAGCCAAGTTTAAACCAACTCAAACAGA GCCAACAAAAATCCGAAGCAGAAAACTGGAACAAGTCATGTGAAAGTACTCCATCTACTATGGGATACAC GTCAAACTGGATTTCTTCTCTCTCCCAAATTCGTGGAGCTGCCCCAACTTCCTTGCCACAAGGACTCCCA CTCCCTTCATTTCATGGGCCATTAGCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGGTG CCGCAGGTTATCCAGTACAGTGGGTAGGAATTTCTGGACCAGCACAACAGTCTGTAGTGCTTCCTACCCA ATCTGGGGGACTCTTCCAGCCCGGGATGAATTTGCAGTCATTTCCAGCTCCACCAGTGCAGAATCCAGCT TCAATCCCTCCTGGTCCTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231872 representing NM_001271678 Red=Cloning site Green=Tags(s) MATDSGEPASTEDSEKPDGVSFENRAARAVAPLTVEARIKEKYSTFSASGENIERKRFFRKSVEMTEDDK VAESSRRDERKAATNISRVDKVPTNVLRGGQEVKYEQCSKATSESSKDCFKEKTEKEMEEEAEMKAVATS PSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYD SWESTLKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNI FITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSEC QNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIKDLLNHAFFAEDTGLRVELAEEDDC SNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLEADTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTLI KKIREKKPAGCLEERRDSQCKYVRNVLPQQQTATLQPTPGPHTAAEYEETEVDQHVRQQFLQGKPQQQSS SVRGDTSSEPTAGPVLHSDTSSHPTVAYSSNQTTSSQEQPKLTQSPVLPVVQGQSSVMPIYAAGVGVVSQ SQISPLTIQKVSQIKPVSQPIGAEQQATLQNPDFVRSLNQDVTSVKENTNNPDTPSGNGKQDRNKQRRAS CPRPEKGTKFQLTVLQVSVSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLENEKEKFV EELRAIVGQAQEILHVHSAVEKSIGVDSVALESNSNQTGSSEQVLINSASTQTSNESAPQSSPVGRWRFC INQTIKNREAQSPPSLQPSMAMVPGLHPFPSSRNTSNQAISQNTVFTIENNPGHRELFTSKLDHKDVVDG KIGEHASIETEQSSISYQVEDDRQIMTPATDNSNYSAALVCPVPGECEALTSQAGMFMPTYPNQQAAVLA DVHIAYPGESVPIGGNAALTSVLVSSDQKPQSLSVQQPTIDAEFISKEGETTVNTETSSPKAVIATQTPG FEPAVILPATILESDGERPPKMEFADNRIKTLDEKLRNLLYQEHSISSICPESQKDTQSIDSPFSSSAED ILSYSMPEVIAISHCGIQDSPAQSPNFQQTGSKILSNVAASQPAHISVFKKDLNVITSVPSELCLHEMSP DASLPGDPEAYPAAVSSDGTIHLQTGGGYFGLSFTCPSLKNPISRKSWTRKLKSWAYRLRQSTSFFKRSK VRQVETEDKRSAIASDPIPLTREFSSDTRALSRCKAMSGSFQRGRFQVITVPQQQPVKMMSFGKDHRPPF NKTTVQSSEQALTFAEAAVSQLIEVEPAMPTHKASVSSRKLRTLYETFKEDKGDPEQGDIVSYSTACETS VSSVATEKNVTSTTEVSVQSGSEPLDKEKNESTPGKQTCTNEFSATLAGNRKSVTKTRPEGDQYLPLREE QAYAQTQNSLFYSPSSPMSSDNESEIEDEDLKVELQRLREKHIQEVVSLQTQQNKELQELYERLRATKDN KAQSSEVPLSPASPRRPRSFKSKLRSRPQSMTHSDNLVVKDALGVESNTVSCQQSPASKKGMFTDDLHKL VDDWTRETVGHFPSKPSLNQLKQSQQKSEAENWNKSCESTPSTMGYTSNWISSLSQIRGAAPTSLPQGLP LPSFHGPLASYGMPHVCQYNAVGAAGYPVQWVGISGPAQQSVVLPTQSGGLFQPGMNLQSFPAPPVQNPA SIPPGPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271678
ORF Size	5271 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271678.1, NP_001258607.1
RefSeq Size	10400 bp
RefSeq ORF	5274 bp
Locus ID	279561
UniProt ID	Q80XP9
MW	194.4 kDa
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.[UniProtKB/Swiss-Prot Function]

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