Idua (NM_172997) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6030426D08
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230622 representing NM_172997 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACCCCCGCGTCCCTCCTCAGCTATGCTGACGTTTTTTGCTGCGTTCTTGGCCGCGCCCTTGGCGC TGGCTGAGTCACCGTACCTGGTGCGTGTGGACGCAGCCCGCCCGCTGAGGCCTCTGTTGCCCTTCTGGAG GAGCACCGGCTTCTGGAAGTCACCTGGGCAGGGACTTATGTACAACTTCACCCACTTGGATGCATTCTTG GACCTTCTCATGGAGAACCAGCTTCTCCCTGGATTTGAGCTCATGGGCAGTCCTTCTGGGTACTTCACGG ACTTTGATGACAAGCAGCAGGTGTTTGAATGGAAGGACCTGGTTTCTCTCTTGGCCAGGAGATACATTGG TAGGTATGGGCTGACACACGTTTCCAAGTGGAACTTTGAGACTTGGAATGAACCAGACCACCATGACTTT GACAACGTGTCCATGACCACACAAGGCTTCCTGAATTACTATGATGCCTGCTCTGAGGGGCTGCGCATTG CCAGCCCCACTTTGAAGTTGGGTGGTCCTGGGGATTCCTTCCACCCCCTGCCAAGGTCACCAATGTGCTG GAGCCTCCTGGGTCACTGTGCCAATGGAACCAACTTCTTCACTGGCGAGGTGGGCGTGCGTCTGGATTAC ATCTCCCTGCACAAGAAGGGTGCAGGTAGCTCCATCGCCATCCTGGAGCAGGAGATGGCAGTTGTGGAGC AGGTCCAGCAGCTCTTCCCTGAGTTCAAGGATACCCCTATTTACAATGACGAGGCAGACCCTCTGGTGGG CTGGTCCCTGCCACAACCTTGGAGAGCTGATGTGACTTATGCGGCCCTGGTGGTGAAGGTCATTGCACAG CACCAGAACCTGCTGTTTGCCAACAGCAGTTCCTCCATGCGCTATGTGCTCCTCAGCAATGACAATGCCT TCCTGAGCTACCACCCGTACCCTTTCTCCCAGCGCACACTTACTGCTCGATTCCAGGTCAACAATACTCA CCCACCCCACGTGCAGTTGCTGCGAAAGCCAGTACTCACAGTCATGGGGCTCATGGCCCTGTTGGATGGA GAACAACTCTGGGCAGAGGTCTCAAAGGCTGGGGCTGTGTTGGACAGCAATCATACAGTGGGTGTCCTGG CCAGCACCCATCACCCTGAAGGCTCCGCAGCGGCCTGGAGTACCACAGTCCTCATCTACACTAGTGATGA CACCCACGCACACCCCAACCACAGTATCCCTGTGACTCTTCGCCTGCGTGGGGTACCTCCTGGCTTGGAT CTTGTCTACATAGTACTCTACTTAGACAATCAACTCAGCAGCCCCTACAGTGCGTGGCAGCACATGGGCC AGCCAGTCTTCCCCTCTGCAGAGCAGTTCCGACGTATGCGCATGGTGGAGGACCCCGTGGCTGAGGCACC ACGCCCCTTTCCTGCTAGGGGCCGCCTGACCCTACACCGGAAGCTTCCGGTGCCATCACTCCTGCTGGTG CATGTATGCACACGCCCCTTGAAGCCACCTGGGCAGGTCAGCCGGCTCCGTGCACTGCCCCTGACACATG GACAGCTGATTTTGGTCTGGTCAGATGAGCGTGTGGGCTCCAAGTGCCTGTGGACATATGAGATCCAGTT TTCCCAGAAAGGTGAAGAGTATGCCCCAATCAACAGGAGGCCGTCTACTTTTAACCTCTTTGTGTTCAGC CCAGACACAGCTGTGGTCTCTGGCTCCTACCGAGTTCGAGCATTGGATTACTGGGCCCGGCCAGGCCCCT TCTCCGACCCTGTGACTTACCTGGATGTCCCTGCCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230622 representing NM_172997 Red=Cloning site Green=Tags(s) MRPPRPSSAMLTFFAAFLAAPLALAESPYLVRVDAARPLRPLLPFWRSTGFWKSPGQGLMYNFTHLDAFL DLLMENQLLPGFELMGSPSGYFTDFDDKQQVFEWKDLVSLLARRYIGRYGLTHVSKWNFETWNEPDHHDF DNVSMTTQGFLNYYDACSEGLRIASPTLKLGGPGDSFHPLPRSPMCWSLLGHCANGTNFFTGEVGVRLDY ISLHKKGAGSSIAILEQEMAVVEQVQQLFPEFKDTPIYNDEADPLVGWSLPQPWRADVTYAALVVKVIAQ HQNLLFANSSSSMRYVLLSNDNAFLSYHPYPFSQRTLTARFQVNNTHPPHVQLLRKPVLTVMGLMALLDG EQLWAEVSKAGAVLDSNHTVGVLASTHHPEGSAAAWSTTVLIYTSDDTHAHPNHSIPVTLRLRGVPPGLD LVYIVLYLDNQLSSPYSAWQHMGQPVFPSAEQFRRMRMVEDPVAEAPRPFPARGRLTLHRKLPVPSLLLV HVCTRPLKPPGQVSRLRALPLTHGQLILVWSDERVGSKCLWTYEIQFSQKGEEYAPINRRPSTFNLFVFS PDTAVVSGSYRVRALDYWARPGPFSDPVTYLDVPAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_172997
ORF Size	1788 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172997.1, NP_766585.1
RefSeq Size	4289 bp
RefSeq ORF	1791 bp
Locus ID	15932
MW	67.4 kDa

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