Kcnj11 (NM_001204411) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Kir6.2; mBIR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229118 representing NM_001204411 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGGTGGCTCATCGCCTTCGCCCACGGTGACCTGGCCCCCGGAGAGGGCACCAATGTGCCCTGCG TCACAAGCATCCACTCCTTTTCATCTGCCTTCCTTTTCTCCATCGAGGTCCAGGTGACCATTGGTTTCGG CGGGCGCATGGTGACAGAGGAATGTCCCCTGGCCATCCTCATTCTCATTGTGCAGAATATCGTCGGGCTG ATGATCAACGCCATCATGCTGGGCTGCATCTTCATGAAAACGGCCCAGGCCCATCGGCGGGCAGAAACCC TCATCTTCAGCAAGCATGCTGTGATCACCCTGCGCCATGGCCGCCTGTGCTTCATGCTGCGCGTAGGGGA CCTCCGAAAGAGCATGATCATTAGCGCCACCATCCACATGCAGGTGGTGCGCAAGACCACCAGCCCCGAG GGCGAAGTTGTGCCTCTCCACCAGGTAGACATCCCCATGGAGAATGGCGTGGGTGGTAACGGCATCTTCC TGGTGGCCCCACTCATCATCTACCACGTCATCGACTCCAACAGCCCGCTCTACGACCTGGCTCCTAGTGA CCTGCACCACCACCAGGACCTGGAGATCATTGTCATCTTGGAAGGCGTGGTAGAAACCACGGGCATCACC ACCCAGGCCCGCACCTCCTACCTAGCTGACGAGATTCTATGGGGGCAGCGCTTTGTCCCCATTGTGGCCG AGGAGGACGGCCGCTATTCTGTGGACTACTCCAAATTTGGTAACACCATTAAAGTGCCCACACCACTCTG CACAGCCCGCCAGCTTGATGAGGACCGCAGTCTGCTGGATGCCCTGACCCTCGCCTCGTCGCGGGGGCCC CTGCGCAAGCGCAGTGTGGCTGTGGCGAAGGCCAAGCCCAAGTTTAGCATCTCTCCAGATTCCTTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229118 representing NM_001204411 Red=Cloning site Green=Tags(s) MVWWLIAFAHGDLAPGEGTNVPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGL MINAIMLGCIFMKTAQAHRRAETLIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPE GEVVPLHQVDIPMENGVGGNGIFLVAPLIIYHVIDSNSPLYDLAPSDLHHHQDLEIIVILEGVVETTGIT TQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDRSLLDALTLASSRGP LRKRSVAVAKAKPKFSISPDSLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204411
ORF Size	909 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204411.1, NP_001191340.1
RefSeq Size	2667 bp
RefSeq ORF	912 bp
Locus ID	16514
MW	33.7 kDa
Gene Summary	This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.