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Cbl (NM_007619) Mouse Tagged ORF Clone
CAT＃: MR227679
- TrueORF®
Cbl (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma (Cbl)
ORF Plasmid: tGFP
"NM_007619" in other vectors (4)
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CNY 6,688.00
CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4732447J05Rik; c-Cbl; Cbl-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227679 representing NM_007619
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCAACGTGAAGAAGAGCTCGGGCGCCGGCGGCGGCGGCTCTGGGGGCTCGGGAGCGGGCGGCC TGATCGGGCTCATGAAGGACGCCTTCCAGCCGCACCACCACCACCACCACCTCAGCCCGCACCCTCCCTG CACGGTGGACAAGAAGATGGTGGAGAAGTGCTGGAAGCTCATGGACAAGGTGGTGCGGTTGTGTCAAAAC CCAAAGCTGGCGCTCAAGAACAGCCCGCCTTATATCTTAGACCTGCTGCCTGACACCTACCAGCACCTCC GCACTGTCTTGTCAAGATATGAGGGGAAGATGGAGACGCTTGGAGAAAATGAGTATTTCAGGGTGTTCAT GGAAAATTTGATGAAGAAAACTAAGCAGACTATCAGCCTCTTCAAGGAGGGAAAAGAAAGGATGTATGAG GAGAATTCCCAGCCTAGGCGAAACCTGACCAAATTATCCCTGATCTTCAGCCACATGCTGGCAGAACTGA AAGGCATCTTTCCGAGCGGACTCTTCCAAGGAGACACTTTCCGGATTACTAAAGCTGATGCTGCCGAATT TTGGAGAAAAGCTTTTGGTGAAAAGACGATAGTCCCGTGGAAGAGCTTTCGACAGGCCCTGCATGAAGTG CATCCCATCAGTTCTGGGCTGGAGGCCATGGCTCTGAAGTCCACTATTGATCTGACCTGCAATGATTATA TTTCTGTCTTTGAATTTGATATTTTTACACGGCTGTTTCAGCCCTGGTCCTCTTTGCTCAGAAATTGGAA CAGCCTTGCTGTAACTCACCCTGGTTACATGGCTTTCCTGACATACGATGAAGTGAAAGCGCGCCTGCAG AAGTTCATCCACAAACCTGGCAGTTACATCTTTCGGCTGAGCTGTACTCGTTTGGGTCAGTGGGCTATTG GGTATGTTACTGCCGATGGGAACATTCTGCAGACAATCCCACACAATAAACCGCTCTTCCAAGCACTGAT TGATGGCTTCAGGGAAGGCTTCTATTTGTTTCCTGATGGACGAAATCAAAATCCTGACCTGACAGGTTTA TGTGAACCAACTCCTCAAGATCATATCAAAGTAACCCAGGAACAATATGAATTATACTGTGAAATGGGCT CCACATTTCAACTGTGTAAGATATGTGCTGAGAATGATAAGGATGTGAAGATTGAGCCCTGTGGACACCT CATGTGCACATCCTGCCTCACGTCGTGGCAGGAATCAGAAGGTCAGGGCTGTCCTTTTTGCCGATGTGAA ATCAAAGGTACTGAGCCCATCGTGGTGGATCCGTTTGACCCCAGAGGCAGTGGCAGCCTATTAAGGCAAG GAGCAGAAGGTGCTCCTTCCCCAAATTACGACGATGATGATGATGAACGAGCTGATGATTCTCTCTTCAT GATGAAGGAGTTGGCAGGTGCCAAGGTGGAAAGGCCTTCCTCTCCATTCTCCATGGCCCCACAAGCTTCC CTTCCTCCAGTGCCACCAAGACTTGACCTTCTACAGCAGCGAGCACCTGTTCCTGCCAGCACTTCAGTTC TGGGGACTGCTTCCAAGGCTGCTTCTGGCTCCCTTCATAAAGACAAACCATTGCCAATACCTCCCACACT TCGAGATCTTCCACCACCACCCCCTCCAGACCGGCCTTACTCTGTTGGAGCAGAAACAAGGCCTCAGAGA CGCCCTCTGCCTTGTACACCAGGCGATTGTCCATCTAGAGACAAACTGCCCCCTGTCCCTTCTAGCCGCC CAGGGGACTCGTGGTTGTCTCGGCCAATCCCTAAAGTACCAGTAGCTACTCCAAACCCTGGTGATCCTTG GAATGGGAGAGAATTGACCAATCGGCACTCGCTTCCATTCTCATTGCCCTCACAAATGGAACCCAGAGCA GATGTCCCTAGGCTTGGAAGCACATTTAGTCTGGATACCTCTATGACTATGAATAGCAGCCCAGTAGCAG GTCCAGAGAGTGAGCACCCAAAGATCAAGCCTTCCTCGTCTGCCAACGCCATTTACTCTCTGGCTGCCAG GCCTCTTCCTATGCCAAAACTGCCACCTGGGGAGCAAGGGGAAAGTGAAGAGGACACAGAATATATGACT CCCACATCTAGGCCTGTAGGGGTTCAGAAGCCAGAGCCCAAACGGCCGTTAGAGGCAACCCAGAGTTCAC GAGCATGTGACTGTGACCAGCAGATCGACAGCTGTACCTATGAAGCGATGTATAACATCCAGTCCCAAGC ACTGTCTGTAGCAGAAAACAGCGCCTCTGGGGAAGGGAATCTGGCCACAGCTCACACGAGTACTGGCCCT GAGGAATCCGAAAACGAGGATGATGGCTATGATGTGCCTAAGCCACCCGTGCCAGCTGTACTGGCCCGCC GGACCCTGTCTGACATCTCCAATGCCAGCTCCTCCTTTGGCTGGTTGTCTTTGGATGGTGACCCTACAAA CTTCAATGAGGGTTCCCAAGTTCCTGAGCGGCCCCCCAAACCATTCCCTCGGAGAATCAACTCAGAACGA AAAGCCAGTAGCTATCAACAAGGCGGAGGTGCCACTGCTAACCCTGTGGCCACAGCACCCTCACCGCAGC TCTCAAGTGAGATTGAACGCCTCATGAGTCAGGGCTATTCCTACCAGGACATTCAGAAAGCTTTGGTCAT TGCCCACAACAACATTGAGATGGCTAAAAACATCCTCCGGGAATTTGTTTCTATTTCTTCTCCTGCTCAC GTAGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227679 representing NM_007619
Red=Cloning site Green=Tags(s) MAGNVKKSSGAGGGGSGGSGAGGLIGLMKDAFQPHHHHHHLSPHPPCTVDKKMVEKCWKLMDKVVRLCQN PKLALKNSPPYILDLLPDTYQHLRTVLSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYE ENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEV HPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQ KFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGL CEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCE IKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDSLFMMKELAGAKVERPSSPFSMAPQAS LPPVPPRLDLLQQRAPVPASTSVLGTASKAASGSLHKDKPLPIPPTLRDLPPPPPPDRPYSVGAETRPQR RPLPCTPGDCPSRDKLPPVPSSRPGDSWLSRPIPKVPVATPNPGDPWNGRELTNRHSLPFSLPSQMEPRA DVPRLGSTFSLDTSMTMNSSPVAGPESEHPKIKPSSSANAIYSLAARPLPMPKLPPGEQGESEEDTEYMT PTSRPVGVQKPEPKRPLEATQSSRACDCDQQIDSCTYEAMYNIQSQALSVAENSASGEGNLATAHTSTGP EESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTNFNEGSQVPERPPKPFPRRINSER KASSYQQGGGATANPVATAPSPQLSSEIERLMSQGYSYQDIQKALVIAHNNIEMAKNILREFVSISSPAH VAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007619 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007619.2, NP_031645.2 |
| RefSeq Size | 5083 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 12402 |
| UniProt ID | P22682 |
| MW | 101 kDa |
| Gene Summary | Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-737' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3 (PubMed:10393178, PubMed:12649282, PubMed:19265199, PubMed:20100865, PubMed:9653117). In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222572 | Cbl (untagged) - Mouse Casitas B-lineage lymphoma (Cbl), (10ug) |
CNY 11,780.00 |
|
| MG227679 | Cbl (tGFP-tagged) - Mouse Casitas B-lineage lymphoma (Cbl), (10ug) |
CNY 8,288.00 |
|
| MR227679L3 | Lenti ORF clone of Cbl (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma (Cbl) |
CNY 9,980.00 |
|
| MR227679L4 | Lenti ORF clone of Cbl (mGFP-tagged) - Mouse Casitas B-lineage lymphoma (Cbl) |
CNY 9,980.00 |
