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Hnf4a (NM_008261) Mouse Tagged ORF Clone
CAT＃: MR227662
- TrueORF®
Hnf4a (Myc-DDK-tagged) - Mouse hepatic nuclear factor 4, alpha (Hnf4a)
ORF Plasmid: tGFP
"NM_008261" in other vectors (4)
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CNY 5,488.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Hnf; HNF-; HNF-4; Hnf4; Hnf4alpha; MODY; MODY1; Nr; Nr2a1; TCF-14; Tcf14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227662 representing NM_008261
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACTCTCTAAAACCCTTGCCGGCATGGATATGGCCGACTACAGCGCTGCCCTGGACCCAGCCTACA CCACCCTGGAGTTTGAAAATGTGCAGGTGTTGACCATGGGCAATGACACGTCCCCATCTGAAGGTGCCAA CCTCAATTCATCCAACAGCCTGGGCGTCAGTGCCCTGTGCGCCATCTGTGGCGACCGGGCCACCGGCAAA CACTACGGAGCCTCGAGCTGTGACGGCTGCAAGGGGTTCTTCAGGAGGAGCGTGAGGAAGAACCACATGT ACTCCTGCAGGTTTAGCCGACAATGTGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCT TAAGAAGTGCTTCCGGGCTGGCATGAAGAAGGAAGCTGTCCAAAATGAGCGGGACCGGATCAGCACGCGG AGGTCAAGCTACGAGGACAGCAGCCTGCCCTCCATCAACGCGCTCCTGCAGGCAGAGGTTCTGTCCCAGC AGATCACCTCTCCCATCTCTGGGATCAATGGCGACATTCGGGCAAAGAAGATTGCCAACATCACAGACGT GTGTGAGTCTATGAAGGAGCAGCTGCTGGTCCTGGTCGAGTGGGCCAAGTACATCCCGGCCTTCTGCGAA CTCCTTCTGGATGACCAGGTGGCGCTGCTCAGGGCCCACGCCGGTGAGCATCTGCTGCTTGGAGCCACCA AGAGGTCCATGGTGTTTAAGGACGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGA GCTAGCGGAGATGAGCCGTGTGTCCATCCGCATCCTCGATGAGCTGGTCCTGCCCTTCCAAGAGCTGCAG ATTGATGACAATGAATATGCCTGCCTCAAAGCCATCATCTTCTTTGATCCAGATGCCAAGGGGCTGAGTG ACCCGGGCAAGATCAAGCGGCTGCGGTCACAGGTGCAAGTGAGCCTGGAGGATTACATCAACGACCGGCA GTACGACTCTCGGGGCCGCTTTGGAGAGCTGCTGCTGCTGTTGCCCACGCTGCAGAGCATCACCTGGCAG ATGATCGAACAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGC TTCTCGGAGGGTCTGCCAGTGATGCACCCCACACCCACCACCCCCTGCACCCTCACCTGATGCAAGAACA CATGGGCACCAATGTCATTGTTGCTAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTGTGAGTGG CCCCGACCCAGGGGGCAGGCAGCCACTCCCGAGACTCCACAGCCATCACCACCAAGTGGCTCGGGATCTG AATCCTACAAGCTCCTGCCAGGAGCCATCACCACCATCGTCAAGCCTCCCTCTGCCATTCCCCAGCCAAC GATCACCAAGCAAGAAGCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227662 representing NM_008261
Red=Cloning site Green=Tags(s) MRLSKTLAGMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGK HYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTR RSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVEWAKYIPAFCE LLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQ IDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ MIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHTHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEW PRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008261 |
| ORF Size | 1422 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008261.3 |
| RefSeq Size | 4371 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 15378 |
| UniProt ID | P49698 |
| MW | 53.1 kDa |
| Gene Summary | The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Generation of metabolically functional hepatocyte-like cells from dedifferentiated fat cells by Foxa2, Hnf4a, and Sall1 transduction
,Hagiwara, R;Oki, Y;Matsumaru, T;Ibayashi, S;Kano, K;,
Genes Cells
,PubMed ID 33064855
[HNF4A]
|
|
Hepatocyte nuclear factor 4α negatively regulates connective tissue growth factor during liver regeneration
,Zhou, J;Sun, X;Yang, L;Wang, L;Ran, G;Wang, J;Cao, Q;Wu, L;Bryant, A;Ling, C;Pi, L;,
FASEB J.
,PubMed ID 32057145
[HNF4A]
|
|
Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver-Specific Microenvironment as a Drug Testing Platform
,Jin, Y;Kim, J;Lee, J;Min, S;Kim, S;Ahn, D;Kim, Y;Cho, S;,
Adv. Funct. Mater.
[HNF4A]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216460 | Hnf4a (untagged) - Mouse hepatic nuclear factor 4, alpha (Hnf4a), (10ug) |
CNY 5,488.00 |
|
| MG227662 | Hnf4a (tGFP-tagged) - Mouse hepatic nuclear factor 4 alpha (Hnf4a), (10ug) |
CNY 7,088.00 |
|
| MR227662L3 | Lenti ORF clone of Hnf4a (Myc-DDK-tagged) - Mouse hepatic nuclear factor 4, alpha (Hnf4a) |
CNY 6,650.00 |
|
| MR227662L4 | Lenti ORF clone of Hnf4a (mGFP-tagged) - Mouse hepatic nuclear factor 4, alpha (Hnf4a) |
CNY 7,888.00 |
