United States
Germany
Japan
United Kingdom
China
Eomes (NM_001164789) Mouse Tagged ORF Clone
CAT＃: MR227087
- TrueORF®
Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 2
ORF Plasmid: tGFP
"NM_001164789" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C77258; TBR-2; Tbr2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227087 representing NM_001164789
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGGGAGAGCAGCTCCTGGTGAGCTCGGTGAACCTGCCCGGCGCGCACTTCTACTCGCTGGAGA GTGCTCGCGGAGGAGGAGGAGGAGGAGGCGGAGGAGGAGGGGGAGGCGGAGGGAGCGTCAGCCTCCTCCC CGGTGCTGCCCCCTCGCCCCAGAGGCTGGACTTAGACAAAGCGTCCAAGAAGTTTCCGGGCAGTCTCCCG TGCCAGGCGGGGAGCGCAGAACCCGCAGGCGCCGGCGCGGGGGCCCCCGCGGCCATGCTCAGTGACGCGG ACGCTGGGGACACCTTCGGGAGCACCTCGGCGGTGGCCAAGCCCGGGCCCCCGGACGGCCGCAAGGGCTC CCCGTGCGCGGAGGAGGAGCTGCCCTCCGCCGCCACCGCCGCGGCCACCGCGCGCTACTCCATGGACAGC CTGAGCTCCGAGCGCTACTACCTCCCGTCGCCGGGACCGCAGGGCTCCGAGCTCGCCGCGCCCTGCTCGC TCTTCCAGTACCCGGCGGCGGCCGGAGCAGCCCACGGACCCGTGTACCCCGCGTCCAATGGCGCGCGCTA CCCCTACGGCTCCATGCTGCCCCCCGGTGGATTCCCCGCCGCCGTGTGCCCGCCCGCGAGGGCGCAGTTC GGCCCCGCTGCGGGTTCGGGGAGCGGCGCTGGTAGCAGCGGCGGTGGTGCCGGCGGTCCTGGCGCCTATC CCTACGGCCAGGGTTCTCCGCTCTACGGGCCATACGCCGGAACCTCAGCGGCCGGGTCTTGTGGAGGATT GGGGGGCCTAGGGGTGCCTGGCTCCGGCTTCCGCGCCCACGTCTACCTGTGCAACCGGCCCCTATGGCTC AAATTCCACCGGCACCAAACTGAGATGATCATCACCAAACAGGGCAGGCGCATGTTTCCTTTCTTGAGCT TCAACATAAACGGACTCAACCCCACCGCCCACTACAATGTTTTCGTGGAAGTGGTTCTGGCCGACCCTAA CCACTGGCGCTTCCAGGGGGGCAAGTGGGTGACCTGCGGCAAAGCGGACAATAACATGCAGGGCAATAAG ATGTACGTTCACCCAGAATCTCCTAACACTGGCTCCCACTGGATGAGGCAGGAGATTTCCTTTGGGAAGT TAAAACTCACCAATAACAAAGGTGCAAACAACAACAACACACAGATGATAGTGTTGCAGTCTCTGCACAA ATACCAACCGAGGCTGCACATCGTGGAAGTGACAGAGGACGGTGTGGAGGACTTGAATGAACCTTCCAAG ACTCAGACCTTCACCTTCTCAGAGACACAGTTCATCGCTGTGACGGCCTACCAAAACACGGATATCACCC AGCTAAAGATCGACCATAACCCCTTCGCCAAAGGCTTCCGGGACAACTACGATTCATCCCATCAGATTGT CCCTGGAGGTCGGTACGGCGTTCAAAACTTCTTCCCGGAGCCCTTTGTCAACACTTTGCCTCAAGCCCGA TATTATAACGGTGAGAGAACCGTGCCACAGACCAACGGCCTCCTCTCACCCCAACAGAGCGAAGAGGTGG CCAACCCTCCCCAGCGGTGGCTTGTCACGCCTGTCCAGCAACCTGTGACCAACAAGCTAGACATCGGTTC TTATGAATCTGAATATACTTCCAGTACCTTGCTCCCATATGGTATTAAGTCCTTGCCCCTGCAGACATCC CATGCCCTGGGGTATTACCCTGACCCGACCTTCCCTGCTATGGCAGGGTGGGGAGGCCGTGGCGCTTATC AGAGGAAGATGGCAGCTGGACTACCATGGACATCCAGAATGAGCCCACCTGTCTTCCCAGAAGATCAGCT TGCCAAGGAAAAAGTTAAAGAAGAGATTAGTTCCTCCTGGATAGAGACTCCCCCCTCCATCAAGTCTCTA GACTCCAGCGACTCCGGGGTGTACAACAGCGCTTGCAAGAGAAAGCGCCTGTCTCCCAGCACCCCCAGCA ATGGAAACTCGCCCCCCATAAAGTGTGAGGACATTAACACTGAAGAGTACAGTAAAGACACCTCCAAAGG CATGGGGGCTTATTATGCTTTTTACACAAGTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227087 representing NM_001164789
Red=Cloning site Green=Tags(s) MQLGEQLLVSSVNLPGAHFYSLESARGGGGGGGGGGGGGGGSVSLLPGAAPSPQRLDLDKASKKFPGSLP CQAGSAEPAGAGAGAPAAMLSDADAGDTFGSTSAVAKPGPPDGRKGSPCAEEELPSAATAAATARYSMDS LSSERYYLPSPGPQGSELAAPCSLFQYPAAAGAAHGPVYPASNGARYPYGSMLPPGGFPAAVCPPARAQF GPAAGSGSGAGSSGGGAGGPGAYPYGQGSPLYGPYAGTSAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWL KFHRHQTEMIITKQGRRMFPFLSFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNK MYVHPESPNTGSHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSK TQTFTFSETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSSHQIVPGGRYGVQNFFPEPFVNTLPQAR YYNGERTVPQTNGLLSPQQSEEVANPPQRWLVTPVQQPVTNKLDIGSYESEYTSSTLLPYGIKSLPLQTS HALGYYPDPTFPAMAGWGGRGAYQRKMAAGLPWTSRMSPPVFPEDQLAKEKVKEEISSSWIETPPSIKSL DSSDSGVYNSACKRKRLSPSTPSNGNSPPIKCEDINTEEYSKDTSKGMGAYYAFYTSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164789 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164789.1, NP_001158261.1 |
| RefSeq Size | 3674 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 13813 |
| UniProt ID | O54839 |
| MW | 73.1 kDa |
| Gene Summary | Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220492 | Eomes (untagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 2, (10ug) |
CNY 8,840.00 |
|
| MG227087 | Eomes (tGFP-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes) transcript variant 2, (10ug) |
CNY 7,144.00 |
|
| MR227087L3 | Lenti ORF clone of Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 2 |
CNY 8,740.00 |
|
| MR227087L4 | Lenti ORF clone of Eomes (mGFP-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 2 |
CNY 8,740.00 |
