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Prdm1 (NM_007548) Mouse Tagged ORF Clone
CAT＃: MR226824
- TrueORF®
Prdm1 (Myc-DDK-tagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1)
ORF Plasmid: tGFP
"NM_007548" in other vectors (6)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | b2b1765Clo; Blimp-1; Blimp1; PRDI-BF1; ZNFPR1A1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226824 representing NM_007548
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGATCTTCTCTTGGAAAAACGTGTGGGTACGACCTTGGCTGCCCCCAAGTCTAGCTCCGGCTCCG TGAAGTTTCAAGGACTGGCAGAGACTGGGATCATGAAAATGGACATGGAGGACGCTGATATGACTTTGTG GACAGAGGCCGAGTTTGAAGAGAAGTGTACATACATAGTGAACGACCACCCCTGGGATTCCGGCGCTGAC GGGGGTACTTCTGTTCAAGCCGAGGCATCCTTACCAAGGAACCTGCTTTTCAAGTATGCTGCCAACAACA GCAAAGAGGTTATTGGCGTGGTAAGTAAGGAGTACATACCGAAGGGAACACGCTTTGGACCCCTCATCGG TGAAGTCTACACTAATGACACAGTTCCCAAGAATGCCAACAGGAAGTATTTTTGGCGGATCTATTCCAGA GAGGAGTTCCACCACTTCATTGATGGCTTTAATGAGGAGAAAAGCAACTGGATGCGCTACGTGAATCCAG CTCACTCTGCCCGGGAGCAAAACCTGGCTGCCTGTCAGAACGGGATGAACATCTACTTCTACACTATTAA GCCTATCCCTGCCAACCAGGAACTTCTTGTGTGGTATTGTCGGGACTTTGCGGAGAGGCTCCACTACCCT TATCCTGGAGAGCTCACAGTGATAAATCTCACACAAACGGAAAGCAACCCAAAGCAATACAGTAGTGAGA AAAATGAACTCTACCCAAAGAGTGTCCCCAAGAGAGAGTACAGCGTGAAAGAAATTCTAAAACTGGACTC CAATCCCTCCAAAAGGAAGGACATCTACCGTTCCAACATTTCACCCTTCACTTTAGAAAAGGACATGGAT GGCTTTCGGAAAAATGGGAGCCCCGACATGCCCTTCTACCCTCGGGTGGTTTATCCTATCCGGGCACCTC TGCCAGAAGACTTTTTGAAAGCGTCCCTGGCCTATGGGATGGAGAGACCCACCTACATAACTCACAGTCC CCTTCCGTCTTCCACAACTCCAAGTCCCCCTGCGAGCAGCAGCCCGGAGCAGAGCCTTAAGAGCTCCAGC CCCCACAGCAGCCCGGGAAACACGGTGTCACCCCTGGCGCCAGGCCTCCCAGAACACCGGGACTCCTACT CCTACTTGAATGTTTCCTATGGTTCCGAGGGCCTGGGCTCCTACCCTGGCTATGCACCTGCCCCCCACCT CCCACCAGCTTTCATTCCTTCTTACAATGCTCACTACCCCAAGTTCCTGTTGCCACCGTACGGCATTAGT TCCAATGGCTTGAGCACCATGAACAACATCAATGGTATCAACAACTTCAGCCTCTTCCCTAGGTTGTATC CCGTCTACAGTAACCTCCTTAGTGGCAGCAGCCTGCCTCATCCCATGCTCAATCCAGCTTCCCTACCGAG TTCCCTGCCTACCGATGGAGCCCGGAGGCTGCTTCCACCGGAGCACCCCAAAGAGGTGCTTATCCCAGCA CCCCACAGTGCCTTCTCCCTTACCGGGGCTGCAGCCAGCATGAAGGACGAGAGTAGTCCCCCCAGCGGAT CTCCAACGGCGGGAACTGCAGCCACGTCAGAACACGTGGTACAACCCAAAGCTACCTCATCAGTGATGGC GGCCCCCAGCACTGACGGAGCCATGAATCTCATTAAAAACAAACGAAACATGACTGGTTACAAGACTCTT CCTTACCCTCTGAAGAAACAGAATGGCAAGATCAAGTATGAGTGCAATGTCTGTGCCAAGACGTTCGGTC AGCTCTCCAACCTGAAGGTCCACCTGAGAGTGCACAGTGGAGAACGGCCTTTCAAGTGCCAGACCTGCAA CAAGGGTTTTACTCAGCTCGCCCACCTGCAGAAACACTACTTGGTACACACAGGAGAGAAGCCACATGAG TGCCAGGTCTGCCACAAGAGATTTAGCAGCACAAGCAATCTCAAGACCCACCTTCGATTGCATTCTGGAG AAAAACCTTACCAATGTAAGGTGTGCCCTGCCAAGTTTACGCAATTTGTGCACCTGAAGCTGCACAAGCG ACTGCATACCCGGGAGCGGCCTCACAAGTGTGCCCAGTGTCACAAGAGCTACATCCATCTCTGCAGCCTC AAGGTCCACCTGAAGGGCAACTGCCCTGCGGGCCCAGCTGCTGGGCTGCCTTTGGAGGATCTGACCCGAA TCAATGAAGAAATTGAGAGGTTCGACATCAGCGACAATGCAGACCGTCTTGAGGACATGGAGGACAGTGT CGATGTGACCTCCATGGTGGAGAAGGAGATTCTAGCTGTGGTCAGAAAAGAGAAAGAAGAAACCAGTCTG AAAGTGTCTTTGCAAAGAAACATGGGGAACGGCCTCCTCTCCTCAGGGTGCAGCCTCTATGAGTCATCGG ACCTGTCCCTCATGAAGTTGCCTCACAGCAACCCACTACCTCTGGTGCCTGTAAAGGTCAAACAAGAAAC AGTTGAACCGATGGATCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226824 representing NM_007548
Red=Cloning site Green=Tags(s) MLDLLLEKRVGTTLAAPKSSSGSVKFQGLAETGIMKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGAD GGTSVQAEASLPRNLLFKYAANNSKEVIGVVSKEYIPKGTRFGPLIGEVYTNDTVPKNANRKYFWRIYSR EEFHHFIDGFNEEKSNWMRYVNPAHSAREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHYP YPGELTVINLTQTESNPKQYSSEKNELYPKSVPKREYSVKEILKLDSNPSKRKDIYRSNISPFTLEKDMD GFRKNGSPDMPFYPRVVYPIRAPLPEDFLKASLAYGMERPTYITHSPLPSSTTPSPPASSSPEQSLKSSS PHSSPGNTVSPLAPGLPEHRDSYSYLNVSYGSEGLGSYPGYAPAPHLPPAFIPSYNAHYPKFLLPPYGIS SNGLSTMNNINGINNFSLFPRLYPVYSNLLSGSSLPHPMLNPASLPSSLPTDGARRLLPPEHPKEVLIPA PHSAFSLTGAAASMKDESSPPSGSPTAGTAATSEHVVQPKATSSVMAAPSTDGAMNLIKNKRNMTGYKTL PYPLKKQNGKIKYECNVCAKTFGQLSNLKVHLRVHSGERPFKCQTCNKGFTQLAHLQKHYLVHTGEKPHE CQVCHKRFSSTSNLKTHLRLHSGEKPYQCKVCPAKFTQFVHLKLHKRLHTRERPHKCAQCHKSYIHLCSL KVHLKGNCPAGPAAGLPLEDLTRINEEIERFDISDNADRLEDMEDSVDVTSMVEKEILAVVRKEKEETSL KVSLQRNMGNGLLSSGCSLYESSDLSLMKLPHSNPLPLVPVKVKQETVEPMDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007548 |
| ORF Size | 2469 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007548.4 |
| RefSeq Size | 5143 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 12142 |
| UniProt ID | Q60636 |
| MW | 92.2 kDa |
| Gene Summary | Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs (PubMed:27102484). Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (PubMed:27102484). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (By similarity). Drives the maturation of B-lymphocytes into Ig secreting cells (By similarity). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (PubMed:27102484).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221845 | Prdm1 (untagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1), (10ug) |
CNY 6,032.00 |
|
| MG226824 | Prdm1 (tGFP-tagged) - Mouse PR domain containing 1 with ZNF domain (Prdm1), (10ug) |
CNY 6,370.00 |
|
| MR226824L1 | Lenti ORF clone of Prdm1 (Myc-DDK-tagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1) |
CNY 7,700.00 |
|
| MR226824L2 | Lenti ORF clone of Prdm1 (mGFP-tagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1) |
CNY 7,700.00 |
|
| MR226824L3 | Lenti ORF clone of Prdm1 (Myc-DDK-tagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1) |
CNY 7,700.00 |
|
| MR226824L4 | Lenti ORF clone of Prdm1 (mGFP-tagged) - Mouse PR domain containing 1, with ZNF domain (Prdm1) |
CNY 7,700.00 |
