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Tgfbr2 (NM_009371) Mouse Tagged ORF Clone
CAT＃: MR226787
- TrueORF®
Tgfbr2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 1
ORF Plasmid: tGFP
"NM_009371" in other vectors (4)
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CNY 6,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110020H15Rik; AU042018; DNIIR; RIIDN; TbetaR-II; TbetaRII; TBR-II |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226787 representing NM_009371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGGGGGCTGCTCCGGGGCCTGTGGCCGCTGCATATCGTCCTGTGGACGCGCATCGCCAGCACGA TCCCGCCGCACGTTCCCAAGTCGGATGTGGAAATGGAAGCCCAGAAAGATGCATCCATCCACCTAAGCTG TAATAGGACCATCCATCCACTGAAACATTTTAACAGTGATGTCATGGCCAGCGACAATGGCGGTGCGGTC AAGCTTCCACAGCTGTGCAAGTTTTGCGATGTGAGACTGTCCACTTGCGACAACCAGAAGTCCTGCATGA GCAACTGCAGCATCACGGCCATCTGTGAGAAGCCGCATGAAGTCTGCGTGGCCGTGTGGAGGAAGAACGA CAAGAACATTACTCTGGAGACGGTTTGCCACGACCCCAAGCTCACCTACCACGGCTTCACTCTGGAAGAT GCCGCTTCTCCCAAGTGTGTCATGAAGGAAAAGAAAAGGGCGGGCGAGACTTTCTTCATGTGTGCCTGTA ACATGGAAGAGTGCAACGATTACATCATCTTTTCGGAAGAATACACCACCAGCAGTCCCGACCTGTTGTT GGTCATTATCCAAGTGACGGGTGTCAGCCTCCTGCCTCCGCTGGGGATTGCCATAGCTGTCATCATCATC TTCTACTGCTACCGTGTCCACCGGCAGCAGAAGCTGAGCCCGTCCTGGGAGAGCAGCAAGCCCCGGAAAC TGATGGATTTCAGTGACAATTGTGCCATCATCCTGGAGGACGACCGCTCCGACATCAGCTCCACGTGCGC CAACAACATCAACCACAACACGGAGCTGCTGCCCATCGAGCTGGACACGCTGGTGGGGAAGGGCCGCTTC GCCGAGGTCTACAAGGCCAAGCTGAAGCAGAACACCTCAGAGCAGTTTGAGACCGTGGCTGTCAAGATCT TCCCCTACGAGGAGTACTCCTCGTGGAAAACAGAGAAGGACATCTTCTCCGATATCAACCTGAAGCATGA GAACATCCTGCAGTTCCTGACGGCCGAGGAGCGGAAGACAGAGCTGGGCAAGCAGTACTGGCTGATCACG GCGTTCCACGCGAAGGGCAACCTGCAGGAGTACCTCACGAGGCATGTCATCAGCTGGGAGGACCTGAGGA AGCTGGGCAGCTCCCTGGCCCGGGGCATCGCTCATCTCCACAGTGACCACACTCCTTGTGGGAGGCCCAA GATGCCCATTGTTCACAGGGACCTCAAGAGCTCTAACATCCTAGTGAAGAACGACTTGACCTGTTGCCTG TGTGACTTCGGGCTGTCCTTGCGCCTGGACCCTACTCTGTCTGTGGATGACCTGGCCAACAGCGGGCAGG TGGGAACGGCAAGATACATGGCCCCGGAAGTTCTAGAATCCAGGATGAATCTGGAAAACGTGGAGTCGTT CAAGCAGACGGATGTCTACTCCATGGCTCTGGTACTCTGGGAAATGACGTCCCGCTGCAATGCTGTGGGA GAAGTGAAGGATTACGAGCCCCCATTTGGTTCCAAGGTGCGGGAGCACCCCTGTGTGGAGAGCATGAAAG ACAGTGTGCTGAGAGACCGAGGGCGGCCGGAAATTCCCAGCTTCTGGCTCAACCACCAGGGCATCCAGAT CGTGTGTGAGACTTTGACCGAGTGCTGGGACCATGACCCCGAAGCCCGTCTCACAGCACAGTGTGTGGCA GAGCGCTTCAGTGAGCTGGAGCATCCGGAGAGACTCTCTGGGAGGAGCTGCTCCCAGGAGAAGATTCCAG AAGATGGCTCGCTGAACACTACCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226787 representing NM_009371
Red=Cloning site Green=Tags(s) MGRGLLRGLWPLHIVLWTRIASTIPPHVPKSDVEMEAQKDASIHLSCNRTIHPLKHFNSDVMASDNGGAV KLPQLCKFCDVRLSTCDNQKSCMSNCSITAICEKPHEVCVAVWRKNDKNITLETVCHDPKLTYHGFTLED AASPKCVMKEKKRAGETFFMCACNMEECNDYIIFSEEYTTSSPDLLLVIIQVTGVSLLPPLGIAIAVIII FYCYRVHRQQKLSPSWESSKPRKLMDFSDNCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRF AEVYKAKLKQNTSEQFETVAVKIFPYEEYSSWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLIT AFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCL CDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVG EVKDYEPPFGSKVREHPCVESMKDSVLRDRGRPEIPSFWLNHQGIQIVCETLTECWDHDPEARLTAQCVA ERFSELEHPERLSGRSCSQEKIPEDGSLNTTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009371 |
| ORF Size | 1776 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009371.3, NP_033397.3 |
| RefSeq Size | 4803 bp |
| RefSeq ORF | 1779 bp |
| Locus ID | 21813 |
| UniProt ID | Q62312 |
| MW | 67.6 kDa |
| Gene Summary | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219401 | Tgfbr2 (untagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 1, (10ug) |
CNY 6,624.00 |
|
| MG226787 | Tgfbr2 (tGFP-tagged) - Mouse transforming growth factor beta receptor II (Tgfbr2) transcript variant 1, (10ug) |
CNY 8,208.00 |
|
| MR226787L3 | Lenti ORF clone of Tgfbr2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 1 |
CNY 7,790.00 |
|
| MR226787L4 | Lenti ORF clone of Tgfbr2 (mGFP-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 1 |
CNY 9,008.00 |
