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Usp19 (NM_145407) Mouse Tagged ORF Clone
CAT＃: MR226430
- TrueORF®
Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 5
ORF Plasmid: tGFP
"NM_145407" in other vectors (4)
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CNY 12,920.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430421I07Rik; AI047774; Zmynd9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226430 representing NM_145407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCAGGGGCCAGTGCTACAGGGCCCAGGAGGGGGCCGCCAGGACTGGAAGAGGCCACTAGTAAGA AGAAACAGAAGGATCGAGCAAACCTGGAAAGTAAAGATGGAGATGCTAGGAGAGTGTCCCTTCCTCGAAA GGAACCAACCAAAGATGAATTGTTGCTCGATTGGAGGCAGAGTGCAGATGAGGTGATTGTTAAGCTGCGC GTGGGAACAGGTCCCGTACGTCTGGAGGATGTAGATGCTGCGTTCACAGACACGGACTGTGTGGTGAGGC TTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTGAAATACAAAGTTCTTGCACCAAAGTGCAGGC TCGCAAGGGTGGTCTTCTACAGCTAGTACTACCCAAGAAGGTGCCTCTGCTCACGTGGCCCTCTCTCCTG AAACCTCTGGGAACCCAAGAGCTGGTGCCAGGTTTGCAGTGCCAGGAGAACGGGCAAGAGCTGTCTCCCA TTGCCCTGGAGCCAGGCTCTGAGCCCCGCAGAGCTAAACAGGAAGCCCGAAACCAGAAGCGGGCCCAGGG CCGTGGTGAGGTAGGCTCGGGGGCTGGCCCTGGGACACAGGCAGGGCCCAGCGCCAAGAGGGCTGTTCAC CTCCGCAGAGGGCCAGAAGGGGAAGGGTCCATGGATGGCCCCGGCCCCCAGGGTGATGCCCCGTCTTTCC TGTCTGACTCAGCTACCCAGGTTGAGGCTGAGGAGAAGCTCTGTGCTCCACCAATGAACACTCAAACAAG TCTCTTGAGCTCAGAGAAGAGTTTAGCCCTTCTGACAGTAGAGAAGACAGTGTCCCCCAGGAATGACCCA GTCGCCCCGGTTATGGTCCAGGACAGAGACCCTGAGCCTGAGCAAGAAGACCAAGTCAAAGAGGAGATGG CACTTGGGGCTGATCCTACAGCCTTGGTGGAGGAACCAGAGTCTATGGTGAACCTGGCATTTGTCAAGAA CGACTCGTATGAGAAGGGCCCGGATTCGGTGGTGGTGCACGTGTACGTGAAGGAGATCCGCAGGGACAGC TCCCGAGTGCTCTTCCGAGAGCAGGACTTCACACTGATCTTCCAGACCAGAAACTTGATTGAACCAGAGC AGTGTACGTTCTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCGCTGGGG GGGACTGGAGGCCCCTGCTACACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGCCCAACC CCTTTGGATTCAACCCCTCCAGGAGGTGGCCCCCACCCCCTGACAGGCCAGGAGGAAGCCAGGGCTGTGG AGAAGGAAAAACCCAAGGCTCGATCAGAGGACTCAGGGCTGGATGGTGTGGTGGCCCGCACCCCCTTGGA GCATGTAGCCCCAAAGCCAGACCCACACTTGGCCTCGCCCAAACCCACGTGTATGGTGCCTCCAATGCCG CACAGTCCAGTTAGTGGGGATAGTGTGGAGGAGGACGAAGAGGAAGAGAAGAAGGTGTGCCTGCCAGGCT TCACTGGCCTTGTCAACTTAGGGAACACCTGCTTCATGAATAGCGTCATTCAGTCTTTGTCCAACACTCG GGAACTTCGTGACTTCTTTCACGACCGATCCTTTGAAGCTGAGATTAACTACAATAACCCATTGGGGACT GGTGGGCGCCTCGCCATTGGCTTTGCTGTGCTGCTCCGGGCCCTATGGAAGGGTACTCACCAAGCCTTTC AGCCCTCCAAGCTAAAGGCCATTGTGGCAAGCAAGGCCAGCCAGTTCACAGGCTATGCACAGCATGATGC TCAAGAGTTCATGGCTTTCTTGTTGGATGGGCTACATGAAGACCTCAATCGAATCCAAAACAAACCCTAC ACAGAGACTGTGGACTCGGACGGGCGGCCCGATGAGGTGGTAGCCGAGGAAGCATGGCAGCGGCACAAGA TGAGAAATGATTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCCTGTGTG TGCCAAGGTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAAAAGGTT CTCCCCATATTTTATTTTGCCAGGGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGTGTCAGCAAGG AGAACTCCAGCGCGAGTGAAGTGTTGGACTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAACCTGCG CCTAGCCGAGGTAATTAAGAACCGTTTCCACCGTGTTTTCTTGCCCTCCCACTCACTGGACGCTGTGTCC CCCACGGACGTGCTCCTCTGCTTTGAGCTGCTCTCCCCAGAACTGGCTAAGGAGCGGGTAGTAGTGCTGG AGGTGCAGCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGCGCAGCCTGCCAGCGGAAGCAGCA ATCAGAAGAAGAAAAGCTGAAGCGCTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTCTGCCAG AAAACCCATTGGCCTGACCACAAAGGCCTCTGCCGCCCTGAGAACATTGGCTACCCCTTCCTGGTCAGTG TGCCTGCTTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTCTGTGAG TGTATTCCAACCGCCCTTCCAGCCTGGCCGCATGGCTTTGGAATCGCAGAGCCCTGGCTGTACCACGTTG CTTTCAACCAGCTCTCTGGAGGCTGGGGACAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCCAGCTGG TGACCCCTGTGGCTGAAGGGGATACAGGGGCTCACCGAGTATGGCCGCCTGCTGATAGGGGTCCTGTGCC TAGCACCAGTGGACTCTCTTCTGAGATGCTGGCCAGTGGGCCTATCGAAGGTTGTCCCTTGCTTGCTGGT GAGAGGGTATCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAACACTCAAGTGAATCTGTGAATACCCACA CGCCCCAGTTCTTCATCTATAAAATTGATGCATCAAACCGTGAGCAGCGGCTGGAGGACAAAGGGGAGAC ACCATTGGAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAATGAACGCCTGCAGGAGTTT GTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAAGATCCAGGCTCTGCTGGTGAGGCTGCCCGTGCTG GCCACTTCACCCTGGACCAGTGCCTCAACCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGAGGCCTG GTACTGCCCACAGTGCAAACAGCATCGTGAGGCCTCCAAACAGCTGCTGTTGTGGCGCCTACCGAACGTG CTGATTGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTCATTTGGCGAGACAAGATCAATGACTTGGTGG AGTTTCCTGTTCGGAACCTGGACTTGAGCAAGTTCTGTATCGGTCAGAAAGAGGAGCAGCTGCCTAGCTA TGACCTGTATGCTGTCATCAACCACTACGGAGGCATGATCGGTGGCCACTATACTGCTTGTGCACGGCTG CCCAATGATCGCAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGACAACAG TAGACGAAAGCCAGGTCGTGACGCGCTATGCCTATGTTCTCTTCTACCGTCGTCGGAACTCTCCTGTGGA GAGACCCCCCAGGGCAAGTCACTCTGAACACCACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGC CAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCCCCCACGCGGACAGCCCCTGAACGCTTCGCCCCCC CTGTGGACCGCCCAGCCCCCACGTACAGCAACATGGAGGAGGTCGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226430 representing NM_145407
Red=Cloning site Green=Tags(s) MSAGASATGPRRGPPGLEEATSKKKQKDRANLESKDGDARRVSLPRKEPTKDELLLDWRQSADEVIVKLR VGTGPVRLEDVDAAFTDTDCVVRLPDGRQWGGVFFAEIQSSCTKVQARKGGLLQLVLPKKVPLLTWPSLL KPLGTQELVPGLQCQENGQELSPIALEPGSEPRRAKQEARNQKRAQGRGEVGSGAGPGTQAGPSAKRAVH LRRGPEGEGSMDGPGPQGDAPSFLSDSATQVEAEEKLCAPPMNTQTSLLSSEKSLALLTVEKTVSPRNDP VAPVMVQDRDPEPEQEDQVKEEMALGADPTALVEEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEIRRDS SRVLFREQDFTLIFQTRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPATRGAVGGAKVAVPTGPT PLDSTPPGGGPHPLTGQEEARAVEKEKPKARSEDSGLDGVVARTPLEHVAPKPDPHLASPKPTCMVPPMP HSPVSGDSVEEDEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRSFEAEINYNNPLGT GGRLAIGFAVLLRALWKGTHQAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPY TETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPFLYLPVPLPQKQKV LPIFYFAREPHSKPIKFLVSVSKENSSASEVLDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLDAVS PTDVLLCFELLSPELAKERVVVLEVQQRPQVPSIPISKCAACQRKQQSEEEKLKRCTRCYRVGYCNQFCQ KTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGRMALESQSPGCTTL LSTSSLEAGDSEREPIQPSELQLVTPVAEGDTGAHRVWPPADRGPVPSTSGLSSEMLASGPIEGCPLLAG ERVSRPEAAVPGYQHSSESVNTHTPQFFIYKIDASNREQRLEDKGETPLELGDDCSLALVWRNNERLQEF VLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRLPNV LIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYGGMIGGHYTACARL PNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERPPRASHSEHHPDLGPAAEAAAS QGLGPGQAPEVAPTRTAPERFAPPVDRPAPTYSNMEEVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145407 |
| ORF Size | 3897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145407.3, NP_663382.2 |
| RefSeq Size | 4653 bp |
| RefSeq ORF | 3900 bp |
| Locus ID | 71472 |
| UniProt ID | Q3UJD6 |
| MW | 143.9 kDa |
| Gene Summary | Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (By similarity). Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains (By similarity). Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224189 | Usp19 (untagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 5, (10ug) |
CNY 16,720.00 |
|
| MG226430 | Usp19 (tGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19) transcript variant 5, (10ug) |
CNY 14,250.00 |
|
| MR226430L3 | Lenti ORF clone of Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 5 |
CNY 14,820.00 |
|
| MR226430L4 | Lenti ORF clone of Usp19 (mGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 5 |
CNY 14,820.00 |
