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Dhx36 (NM_028136) Mouse Tagged ORF Clone
CAT＃: MR226419
- TrueORF®
Dhx36 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36)
ORF Plasmid: tGFP
"NM_028136" in other vectors (4)
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CNY 6,968.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810407E23Rik; AI452301; AU022184; Ddx36; mKIAA1488 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226419 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTACGACTATCATCAGAGCTGGAGCCGCGATGGGGGCCCACGGGGCTCCGGCCAGGGCTCCAGCG GCGGCGGCGGAGGGAGCCGGGGCTCGGGCGGCGGCGGAGGGGGCCGCGGCGGCCGGGGCCGGCATCCCGC ACACCTTAAGGGTCGCGAGATCGGCCTGTGGTACGCCAAGAAGCAGACGCAGAAGAACAAGGAGGCTGAG AGGCAGGAGAGAGCTGTAGTGCACATGGATGAACGTCGAGAAGAGCAAATTGTGCAGCTGCTGAATTCAG TCCAAGCTAAGACTGACAAAGATTCAGAAGCGCAGATATCCTGGTTTGCTCCTGAGGATCATGGGTATGG TACTGAAGTTTCTTCAGAGAAAAAAATAAACTCAGAGAAGAAACTTGACAACCAGGAAAAGAAATTGCTA AACCAAGAAAAAAAGACATTTAGGATCACAGACAAATCATATATTGACCGAGATACTGAGTATTTATTGC AAGAAAATGAGCCAAACCTAAGCTTAGATCAACATTTACTGGAAGATTTACAAAGGAAAAAAACTGACCC TCGATATATAGAGATGCAGCGTTTCAGAAAAAAGCTGCCTTCATATGGAATGCAGAAGGAGCTGGTAAAT CTAATCAATAACCATCAGGTGACAGTAATAAGTGGTGAAACTGGTTGTGGCAAAACCACTCAGGTTACGC AGTTCATCTTGGATAACTACATCGAAAGAGGAAAAGGGTCTGCCTGCAGAATAGTGTGTACTCAGCCAAG AAGAATTAGTGCCATTTCAGTCGCTGAGAGAGTGGCCACAGAAAGGGCAGAGTCTTGTGGCAATGGTAAT AGTACTGGATACCAGATTCGTCTTCAAAGTCGGTTGCCAAGGAAACAAGGTTCTATCTTATACTGCACAA CAGGAATCATTCTTCAGTGGCTCCAGTCCGACTCACGTTTGTCCAGTGTTAGTCATATTGTACTTGATGA AATTCATGAAAGGAATCTACAGTCGGATGTTTTAATGACTGTTATTAAAGATCTTCTCCATTTTCGATCT GATCTCAAAGTAATATTGATGAGTGCAACTTTGAATGCTGAGAAATTTTCAGAATATTTTGGTAACTGTC CAATGATACATATACCCGGGTTTACTTTTCCAGTTGTGGAATATCTTTTGGAAGATATCATTGAAAAAAT AAGATATGTTCCAGACCAAAAAGAACATAGATCCCAGTTCAAGAGGGGTTTCATGCAGGGTCATGTAAAT AGACAAGAAAAAGAAGAAAAAGAGGCCATCTATAAGGAACGCTGGCCAGCGTATATAAAGGAACTGCGGA CAAGATACTCTGCAAGTACCGTAGATGTTTTGCAAATGATGGATGATGATAAAGTTGATCTGAATTTGAT TGCTGCCCTTATTCGATACATTGTTTTGGAAGAAGAGGATGGTGCAATATTGGTCTTTCTACCAGGCTGG GACAATATCAGTACTTTGCATGATCTCTTGATGTCACAAGTGATGTTTAAATCAGATAAGTTTCTCATTA TACCTTTACATTCACTGATGCCTACCGTCAACCAGACACAGGTATTTAAAAAAACTCCTCCCGGTGTTCG GAAAATAGTAATTGCTACCAACATTGCAGAGACTAGCATCACCATAGATGATGTGGTTTATGTAATAGAT GGAGGAAAAATTAAAGAAACACACTTTGACACACAGAACAACATCAGTACCATGTCTGCTGAGTGGGTTA GTAAAGCTAATGCCAAACAGAGGAAAGGGAGAGCTGGAAGAGTTCAGCCTGGTCATTGTTATCATCTGTA TAATGGTCTTAGAGCAAGTCTTCTAGATGACTACCAACTACCAGAAATTTTGAGAACTCCTTTGGAAGAA CTCTGTTTGCAAATAAAGATCTTGAGGCTGGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGATCCAC CATCAAATGAGGCAGTGGTGCTCTCCATAAAACACCTAATGGAACTGAGTGCTTTGGATAAGCAAGAAGA ATTAACACCTCTTGGTGTCCATTTAGCCCGACTGCCTGTTGAGCCACATATTGGAAAAATGATTCTCTTT GGAGCTTTGTTCTGCTGCTTAGATCCAGTTCTCACCATTGCTGCCAGTCTCAGCTTTAAAGATCCCTTTG TCATTCCATTGGGAAAAGAAAAGATTGCAGATGCAAGAAGAAAAGAACTGGCAAAGGAAACTAGAAGTGA TCACCTGACAGTTGTGAATGCATTTGAGGGCTGGGAAGAAGCCAAACGACGTGGTTTCAGGTATGAAAAG GACTACTGCTGGGAATATTTTCTGTCTTCAAACACACTACAGATGCTGCATAACATGAAGGGACAGTTTG CTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGTAGAAGTCCCAAAGATCCAAAAGCTAATATAAATTC AGATAATGAGAAGATAATTAAAGCTGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATCCGACTA AATTTGGGTAAAAAGAGGAAAATGGTAAAAGTTCACACGAAGTCTGACGGCCTGGTTTCTATTCATCCTA AGTCTGTCAACGTGGAGCAGACAGACTTCCACTACAACTGGCTTATCTATCACCTGAAGATGAGAACAAG CAGTATATACTTGTACGACTGTACAGAAGTGTCACCATACTGCCTCCTGTTCTTTGGAGGAGATATTTCC ATCCAGAAAGATAAGGATCAGGAAATTATTGCTGTAGATGAGTGGATTGTGTTTCAGTCTCCAGAAAGAA TTGCCCATCTTGTTAAGGGACTAAGAAAGGAACTGGATAGCCTTCTACAAGAGAAGATTGAAAGCCCTCA TCCTGTAGACTGGGACGACACTAAGTCAAGAGACTGTGCAGTACTGTCAGCTATTCTAGACTTGATCAAA ACTCAAGAAAAGGCTACTCCAAGGAACTTGCCACCACGGTCACAGGACGGATATTATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226419 protein sequence
Red=Cloning site Green=Tags(s) MSYDYHQSWSRDGGPRGSGQGSSGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAE RQERAVVHMDERREEQIVQLLNSVQAKTDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLL NQEKKTFRITDKSYIDRDTEYLLQENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVN LINNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGN STGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRS DLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVN RQEKEEKEAIYKERWPAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGW DNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVID GGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEE LCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILF GALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEK DYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRL NLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDIS IQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPVDWDDTKSRDCAVLSAILDLIK TQEKATPRNLPPRSQDGYYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028136 |
| ORF Size | 3003 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028136.1, NM_028136.2, NP_082412.2 |
| RefSeq Size | 4975 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 72162 |
| UniProt ID | Q8VHK9 |
| MW | 113.8 kDa |
| Gene Summary | Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:25611385). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:21703541, PubMed:21590736). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA) (By similarity). Plays a role in genomic integrity (By similarity). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (By similarity). Plays a role in transcriptional regulation. Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:25611385) (By similarity). Plays a role in post-transcriptional regulation (By similarity). Unwinds a G4-RNA structure located in the 3' UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (By similarity). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (By similarity). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3' UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (By similarity). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3' UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (By similarity). Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (By similarity). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541). Required for the early embryonic development and hematopoiesis (PubMed:22422825). Involved in the regulation of cardioblast differentiation and proliferation during heart development (PubMed:26489465). Involved in spermatogonia differentiation (PubMed:25611385). May play a role in ossification (PubMed:21590736).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223152 | Dhx36 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), (10ug) |
CNY 12,920.00 |
|
| MG226419 | Dhx36 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), (10ug) |
CNY 9,220.00 |
|
| MR226419L3 | Lenti ORF clone of Dhx36 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36) |
CNY 9,368.00 |
|
| MR226419L4 | Lenti ORF clone of Dhx36 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36) |
CNY 10,260.00 |
