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Hic1 (NM_010430) Mouse Tagged ORF Clone
CAT＃: MR226267
- TrueORF®
Hic1 (Myc-DDK-tagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 1
ORF Plasmid: tGFP
"NM_010430" in other vectors (4)
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CNY 8,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408311; HIC-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226267 representing NM_010430
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTACAAGAATGCGCCACCATCCCTGGCTTAAGAGTTCCTCATTTGAAGACAGAACCTTCCCCTCCC CCCCCCCGAATTGGCGCGATATAACATATAAATATGTACTCGGGAGCACTCAGCACAAAACCCCCAGGTT TATGGGACTCCCAGGTCCGATATCTGAGTCTCCAGGTGGGCACATCTGGAAGCGCGCGAACCTGCAAGAT CTGCTCAGCTCCGCATCTGCAAGCCTCTTGGCCCAGGTGTGCGCCAGAGGTCGCAGCCCAGCCGCACACA GTCCCCGCGTGGCCGCGAGGTGGCGCCATGGCCGCGGCAGCGTCTGCCGGTTCGGGCCGCTCCAGATAAG AGTGTGCGGAAAGCGCGGAGGGGCTGAGACGCGACCAGGACGCGGGGAGGACGGACCGGCAAGACAGACC GACCGGGGGCCCGGCGGGCGGAGGGCAGCGCACTGCAGCCACGTCCCCCCCTGGATCCGCCGGCAGCCGG GCCCGGGGCTTCCGACATGCCCCCCAGGAGAGTGTGCTGGGCAGACGATGCTGGACACGATGGAGGCGCC TGGCCATTCGAGGCAGCTACTGCTGCAGCTCAACAATCAGCGCACCAAGGGCTTCTTGTGCGACGTGATC ATCGTGGTGCAGAATGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCCTACCTCAAGT CCCTGGTGGTGCATGACAACCTGCTAAACCTGGACCATGACATGGTGAGCCCGGCCGTGTTCCGCCTGGT GCTGGACTTCATCTACACCGGCCGCCTGACTGACAGTGTTGAGGCCGCAGCAGCAGCAGCGGTGGCCCCG GGCGCGGAGCCGAGCCTGGGCGCTGTGCTGGCTGCTGCCAGCTACCTGCAGATCCCTGACCTCGTGGCTC TGTGCAAGAAGCGCCTCAAACGCCACGGCAAGTACTGCCACCTGCGGGGAGGAGGCAGCGGCGGCGGCGG CTACGCTCCTTACGGGCGGCCCGGCCGGGGCTTGAGGGCTGCCACGCCCGTCATCCAGGCTTGCTACTCG TCCCCGGCCGGGCCACCGCCGCCGCCTGCCGCCGAGCCGCCGTCGGGCCCCGATGCAGCCGTCAACACCC ACTGCGCTGAGCTATATGCTTCAGGCCCGGGCCCAGCAGCCTCACTCTGCGCCCCGGAGCGTCGCTGCTC CCCGCTTTGCGGCCTGGATCTGTCCAAGAAGAGCCCGCCAGGTTCCTCGGTCCCCGAGCGACCGCTTAGT GAGCGCGAACTGCCTCCACGCCCGGATAGCCCTCCCGGTGCGGGGCCCGCAGTCTACAAGGAGCCATCAC TCGCCCTGCCCCCGCTGCCGCCTTTGCCCTTCCAAAAGCTGGAGGAGGCCGTACCGACTCCAGACCCGTT TCGAGGAAGCGGTGGCAGTCCGGGACCCGAGCCCCCCGGCCGCCCCGACGGCTCCAGCCTCCTCTACCGC TGGATGAAGCACGAGCCAGGCCTGGGTAGTTATGGCGATGAACTGGTCCGGGATCGAGGCTCCCCGGGTG AGCGCCTGGAGGAGCGCGGTGGGGATCCTGCCGCCTCACCCGGGGGCCCCCCGCTGGGCCTGGTACCCCC GCCACGCTACCCCGGGAGCCTGGACGGGCCAGGCACAGGAGCAGACGGCGACGACTATAAGAGCAGCAGC GAGGAGACCGGTAGCAGCGAAGACCCCAGCCCACCCGGCGGCCACCTGGAGGGCTACCCATGCCCGCACT TGGCTTATGGTGAGCCTGAGAGCTTTGGTGACAACCTGTACGTGTGCATCCCATGTGGCAAAGGCTTCCC CAGCTCGGAACAGCTGAATGCACACGTGGAGGCTCACGTAGAGGAAGAAGAGGCGTTATATGGCAGGGCA GAGGCTGCTGAGGTGGCTGCTGGGGCCGCCGGCCTTGGGCCCCCCTTTGGTGGCGGTGGGGACAAGGTCA CTGGGGCGCCGGGCGGACTAGGAGAGCTGCTGCGGCCATACCGCTGCGCGTCCTGCGACAAGAGCTACAA GGACCCGGCCACGCTGAGGCAGCACGAGAAGACACACTGGCTGACAAGGCCCTATCCGTGTACCATCTGC GGAAAGAAGTTCACGCAACGCGGAACCATGACACGCCATATGCGCAGCCACTTGGGCCTGAAGCCCTTTG CGTGCGACGCGTGCGGCATGCGCTTCACCCGCCAGTATCGCCTCACGGAGCACATGCGCATCCACTCTGG AGAGAAGCCCTACGAGTGCCAGGTGTGCGGTGGCAAGTTTGCTCAACAGCGCAACCTCATCAGCCATATG AAGATGCACGCTGTAGGTGGCGCGGCCGGCGCGGCCGGGGCGCTGGCTGGCCTGGGGGGACTACCTGGCG TCCCTGGCCCTGACGGCAAGGGCAAGCTCGACTTCCCTGAGGGTGTCTTTGCTGTGGCCCGCCTCACAGC TGAACAGCTGAGTCTGAAGCAGCAGGACAAGGCAGCTGCTGCCGAGCTGCTGGCGCAGACCACGCACTTC CTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCTAAATTCACTGCTGAGCTAGGACTCA GCCCAGACAAGGCGGCAGAGGTGCTGAGCCAGGGTGCGCACTTGGCCGCAGGACCGGACAGCCGAACCAT CGACCGTTTCTCTCCCACC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >MR226267 representing NM_010430
Red=Cloning site Green=Tags(s) MITRMRHHPWLKSSSFEDRTFPSPPPNWRDITYKYVLGSTQHKTPRFMGLPGPISESPGGHIWKRANLQD LLSSASASLLAQVCARGRSPAAHSPRVAARWRHGRGSVCRFGPLQIRVCGKRGGAETRPGRGEDGPARQT DRGPGGRRAAHCSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVI IVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLTDSVEAAAAAAVAP GAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGSGGGGYAPYGRPGRGLRAATPVIQACYS SPAGPPPPPAAEPPSGPDAAVNTHCAELYASGPGPAASLCAPERRCSPLCGLDLSKKSPPGSSVPERPLS ERELPPRPDSPPGAGPAVYKEPSLALPPLPPLPFQKLEEAVPTPDPFRGSGGSPGPEPPGRPDGSSLLYR WMKHEPGLGSYGDELVRDRGSPGERLEERGGDPAASPGGPPLGLVPPPRYPGSLDGPGTGADGDDYKSSS EETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRA EAAEVAAGAAGLGPPFGGGGDKVTGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTIC GKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHM KMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHF LHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDSRTIDRFSPT TRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010430 |
| ORF Size | 2679 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010430.2, NP_034560.2 |
| RefSeq Size | 4247 bp |
| RefSeq ORF | 2682 bp |
| Locus ID | 15248 |
| MW | 94.8 kDa |
| Gene Summary | Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222411 | Hic1 (untagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 1, (10ug) |
CNY 11,500.00 |
|
| MG226267 | Hic1 (tGFP-tagged) - Mouse hypermethylated in cancer 1 (Hic1) transcript variant 1, (10ug) |
CNY 12,064.00 |
|
| MR226267L3 | Lenti ORF clone of Hic1 (Myc-DDK-tagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 1 |
CNY 10,830.00 |
|
| MR226267L4 | Lenti ORF clone of Hic1 (mGFP-tagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 1 |
CNY 12,864.00 |
