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Srebf2 (NM_033218) Mouse Tagged ORF Clone
CAT＃: MR226021
- TrueORF®
Srebf2 (Myc-DDK-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2)
ORF Plasmid: tGFP
"NM_033218" in other vectors (4)
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CNY 7,872.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI608257; bHLHd2; lop13; nuc; SREBP-2; SREBP2; SREBP2gc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226021 representing NM_033218
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGAGCAGCGAGCTGGGCGTTCTGGAGACCATGGAGACCCTCACGGAGCTGGGCGATGAGCTGA CTCTCGGGGACATCGACGAGATGCTACAGTTTGTCAGCAATCAAGTGGGAGAGTTCCCTGATTTGTTTTC AGAGCAACTTTGTAGCTCCTTCCCTGGAGGTGGCAGCAATGGTGGCAGTGGCAACAACAGCAGTGGCAGA GGCAACAATGGTGGAGCCACAGACCCTGCAGTACAGCGGTCATTCAGCCAGGTCCCATTATCTACCTTCT CTCCCTCAGCTGCCTCCCCTCAGGCTCCGGCCCTGCAGGTCAAAGTCTCTCCTACCCCACCAAGGGCAAC CCCTGTTCTCCAGCCGCGACCCCAGCCCCAGCCCCAACCTCCAGCTCAGCTGCAGCAGCAGACGGTGATG ATCACCCCGACGTTCAGCACCGCTCCGCAGACGAGGATCATCCAGCAGCCTTTGATATACCAGAACGCAG CGACCAGCTTTCAAGTCCTGCAGCCTCAAGTGCAAAGCCTCGTGACGTCCCCGCAGGTGCAGCCCGTCAC CATCCAGCAGCAGGTGCAGACGGTACAGGCCCAGCGGGTGTTGACGCAGACAGCCAATGGCACACTGCAG ACCCTTGCCCCCGCCACGGTACAGACGGTCGCTGCGCCACAGGTGCAGCAGGTCCCGGTCCTGGTGCAAC CTCAGATCATTAAGACAGATTCCCTTGTGTTGACCACGCTGAAGACAGATGGCAGCCCTGTCATGGCTGC AGTCCAGAACCCGGCCCTCACGGCCCTCACTGCCCCCATCCAGACAGCCGCCCTTCAAGTGCCAACCCTG GTGGGCAGCAACGGGACCATTCTGACCACAATGCCTGTGATGATGGGGCAAGAGAAAGTTCCTATCAAGC AAGTGCCTGGCGGCGTGAAGCAGCTGGATCCTCCCAAAGAAGGAGAGAGGCGGACAACACACAATATCAT TGAAAAGCGCTACCGGTCCTCCATCAACGACAAAATCATAGAGTTGAAGGACTTAGTCATGGGGACAGAT GCCAAGATGCACAAGTCTGGCGTTCTGAGGAAGGCCATTGATTACATCAAATATCTGCAGCAGGTCAATC ACAAGCTGCGCCAGGAGAACATGGTGCTGAAGCTGGCCAATCAGAAAAACAAGCTCCTGAAGGGCATCGA CCTGGGCAGTCTGGTGGACAGTGATGTGGACTTGAAAATTGATGACTTTAACCAGAATGTCCTTCTGATG TCTCCGCCGGCCTCCGACTCCGGGTCCCAGGCCGGCTTCTCTCCCTATTCCATTGACTCTGAGCCGGGCA GCCCTCTGCTGGATGACGCAAAGGTCAAGGATGAACCGGACTCTCCTCCTGTGGCACTGGGCATGGTGGA CCGCTCTCGAATCCTCTTATGTGTCCTCACCTTCCTGGGCCTCTCCTTTAACCCCTTGACTTCCTTGCTG CAGTGGGGAGGGGCCCACAACACTGACCAGCACCCATACTCAGGCTCGGGCCGCAGTGTACTGTCACTGG AGTCAGGTGCTGGGGGCTGGTTTGACTGGATGGTTCCAACTCTCCTCCTGTGGCTGGTAAATGGTGTGAT TGTCTTGAGCGTCTTTGTGAAGCTGTTGGTCCACGGGGAGCCGGTGATTCGCCCACACTCACGCCCCTCA GTCACCTTCTGGAGACACCGGAAGCAGGCAGACCTAGACCTCGCCAAAGGTGATTTCGCAGCTGCTGCTG CCAACCTACAAACCTGCTTATCCGTGCTGGGCCGGGCGCTGCCCACCTCCCGCCTGGACTTGGCCTGCAG CCTCTCCTGGAATGTGATCCGCTACAGCCTGCAGAAGCTGCGCCTGGTACGCTGGTTACTCAAGAAGGTC TTCCAGCGCTGGCGGGCTACTCCCGCCACTGCAGCTGGCTTTGAGGATGAGGCTAAGAGCAGCGCAAGGG ATGCGGCACTGGCCTACCACAGGCTGCACCAGCTGCACATCACAGGGAAGCTTCCGGCGGGATCTGCCTG TTCAGATGTGCACATGGCTCTGTGTGCTGTGAACCTGGCCGAGTGTGCGGAGGAGAAAATCCTACCAAGC ACACTGATTGAGATCCACCTGACAGCGGCCATGGGGCTCAAGACCCGCTGTGGAGGCAAGCTGGGCTTCT TGGCTAGCTACTTCTTAAATCGTGCCCAGAGTCTGTGTGGCCCAGAGCATAGCACTGTCCCCGACTCCCT GCGCTGGCTATGCCATCCCTTGGGCCAGAAGTTTTTCATGGAGCGAAGCTGGTCGATCAAGTCAGCAGCC AAGGAGAGCCTGTACTGCGCCCAGAGGAGCCCAGCTGACCCCATTGCCCAGGTACACCAGGCGTTCTGCA AAAACCTGCTAGAAAGAGCGGTGGAGTCCTTGGTGAAACCTCAGGCCAAGAAGAAGGCAGGCGACCAGGA AGAAGAGAGCTGTGAATTTTCCAGTGCTCTAGAGTATTTGAAATTACTTCATTCTTTTGTGGACTCTGTG GGATTTGTGACCTCACCATTCTCCAGCAGTTCCGTGCTCAGGTCGGCCCTTGGCCCCGATGTCATCTGCC GGTGGTGGACGTCCGCAGTCACCATGGCCATCAGCTGGCTCCAGGGAGACGATGCAGCTGTGCGCTCTCG TTTTACTGAAGTAGAGCGGGTCCCCAAGGCCCTGGAAGTGACCGAGAGTCCCTTGGTGAAGGCCGTCTTC TATACCTGTAGAGCCATGCATGCCTCCCTCTCTGGGAAAGCTGACGGGCAGCAGAACTCCTTCTGTCACT GTGAGAGGGCCAGTGGTCACCTGTGGAGCAGTCTCAACGTCAGCGGCACTACTTCTGATCCTTCACTTAA CCATGTGATCCAGCTGTTCACCTGTGACCTGCTACTGTCGCTGAGGACAGCACTCTGGCAAAAGCAGGCC AGTGCCAGCCAGCTCCTGGGTGAGACCTACCATGCATCCGGCACTGAGCTGGCCGGCTTCCAGCGGGACC TGGGCAGCCTTCGAAGGCTGGCCCATAGCTTCCGCCCAGCCTACCGCAAGGTGTTCCTGCATGAAGCCAC TGTGCGCCTGATGGCAGGAGCCAGCCCTACCCGTACACACCAGCTGCTGGAGCATAGCCTACGGCGGCGC CCCACTCAGAACACCAAGCATGGAGAGGTAGACACCTGGCCTGGCCAACGAGAGCGAGCTACTGCCATCC TGCTGGCCTGCCGCCATCTTCCCCTCTCTTTCCTGTCTTCCCCGGGCCAGCGGGCAGTGCTGCTGGCTGA AGCTGCCCGCACCCTGGAGAAGGTGGGTGACCGCCGTTCCTGCAGTGACTGCCAGCAGATGATTGTCAAG CTGGGCGGTGGCACTGCCATCGCTGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226021 representing NM_033218
Red=Cloning site Green=Tags(s) MDESSELGVLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGGGSNGGSGNNSSGR GNNGGATDPAVQRSFSQVPLSTFSPSAASPQAPALQVKVSPTPPRATPVLQPRPQPQPQPPAQLQQQTVM ITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSPQVQPVTIQQQVQTVQAQRVLTQTANGTLQ TLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTAPIQTAALQVPTL VGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLDPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTD AKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDSDVDLKIDDFNQNVLLM SPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLGLSFNPLTSLL QWGGAHNTDQHPYSGSGRSVLSLESGAGGWFDWMVPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRPS VTFWRHRKQADLDLAKGDFAAAAANLQTCLSVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKV FQRWRATPATAAGFEDEAKSSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKILPS TLIEIHLTAAMGLKTRCGGKLGFLASYFLNRAQSLCGPEHSTVPDSLRWLCHPLGQKFFMERSWSIKSAA KESLYCAQRSPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSV GFVTSPFSSSSVLRSALGPDVICRWWTSAVTMAISWLQGDDAAVRSRFTEVERVPKALEVTESPLVKAVF YTCRAMHASLSGKADGQQNSFCHCERASGHLWSSLNVSGTTSDPSLNHVIQLFTCDLLLSLRTALWQKQA SASQLLGETYHASGTELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRR PTQNTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCSDCQQMIVK LGGGTAIAAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033218 |
| ORF Size | 3390 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033218.1, NP_150087.1 |
| RefSeq Size | 4570 bp |
| RefSeq ORF | 3393 bp |
| Locus ID | 20788 |
| UniProt ID | Q3U1N2 |
| MW | 123.4 kDa |
| Gene Summary | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223685 | Srebf2 (untagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), (10ug) |
CNY 8,664.00 |
|
| MG226021 | Srebf2 (tGFP-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2), (10ug) |
CNY 10,830.00 |
|
| MR226021L3 | Lenti ORF clone of Srebf2 (Myc-DDK-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2) |
CNY 11,780.00 |
|
| MR226021L4 | Lenti ORF clone of Srebf2 (mGFP-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2) |
CNY 11,780.00 |
