Vcan (NM_019389) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5430420N07Rik; 9430051N09; Cspg2; DPEAAE; hdf; NG2; PG-M; PG-M(V0); PG-M(V1)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225930 representing NM_019389 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAAAGGCATCTTATGGATGTGCTCAACCTTATTACTAACCCATGCACTACATCAAG CCAAAATGGAAACCAGCCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGGTCCTACCTTGTCATTTTTC AACCTTACCTACCTTACCACCCAATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAGATGGAA GTGGACAAAAATGGAAAAGATATAAAGGAGACGACTGTCTTGGTGGCCCAGAACGGAAATATCAAGATTG GTCAGGACTACAAGGGGCGAGTGTCCGTGCCTACACATCCCGATGATGTAGGTGATGCTTCCCTCACCAT GGTCAAACTCCGGGCTAGTGATGCAGGCGTCTACCGATGTGATGTCATGTATGGGATTGAAGACACTCAG GACACCATGTCACTGGCTGTGGATGGTGTTGTGTTTCACTACAGGGCAGCCACCAGCAGGTACACTCTGA ACTTTGCCGCTGCTCAACAGGCTTGTTTGGATATCGGGGCGGTCATAGCAAGCCCAGAGCAGCTGTTTGC CGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGATCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTACGGAGACATGATGGGGAAGGAAGGGGTTCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGTTATGTGGATCATCTGGATGGCGATGTGTTCCACATCACTGC TCCCAGTAAGTTCACCTTCGAGGAGGCCGAAGCAGAGTGTACAAGCAGGGATGCGAGGCTGGCGACTGTT GGAGAACTTCAGGCAGCTTGGAGAAATGGCTTTGACCAATGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGAGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGTCGATTG AGTGATATGATTGTAAGTGGTCATCCAATAGATTCAGAATCTAAAGAAGAGGAACCTTGCAGTGAAGAAA CAGATCCACTGCATGATCTGTTTGCTGAAATTTTACCCGAGTTACCAGACTCATTTGAAATAGACATATA TCACAGTGAGGAAGATGAAGACGGAGAGGAGGACTGTGTAAATGCAACGGATGTAACAACTACTCCGTCA GTGCAGTATATCAATGGGAAGCAGCTCGTTACCACAGTGCCTAAGGACCCGGAAGCTGCAGAAGCTAGGC GTGGCCAGTACGAAAGTGTTGCACCTTCTCAGAATTTCCCAGATAGTTCTGCAACTGACACCCATCAGTT TATACTAGCAGAAACAGAATCGTCAACTACCATGCAATTTAAGAAATCTAAAGAAGGCACGGAATTGTTA GAAATCACATGGAAACCCGAGACCTACCCTGAAACACCAGACCATGTTTCAAGTGGTGAGCCTGATGTTT TCCCTACTCTCTCATCCCATGATGGTAAAACCACCAGATGGTCAGAGTCCATCACAGAGAGCAGTCCAAA CCTTGAAAATCCAGTGCACAAACAACCTAAGCCTGTCCCTCTGTTTCCTGAAGAGTCTTCAGGAGAGGGT GCCATTGAACAAGCATCCCAGGAAACAATCTTATCTAGGGCTACAGAAGTAGCACTTGGTAAAGAGACAG ACCAAAGTCCTACCTTATCTACCTCTAGCATACTTTCAAGTTCTGTGTCAGTAAATGTCTTGGAGGAAGA ACCACTTACTCTAACAGGAATCTCACAGACTGATGAATCCATGTCTACTATAGAAAGCTGGGTAGAGATA ACACCTAGCCAAACTGTAAAGTTCTCGGAGAGTTCTTCAGCTCCTATCATTGAAGGGTCTGGGGAAGTAG AAGAAAATAAAAATAAAATCTTTAACATGGTAACTGATTTACCACAGAGAGATCCTACAGATACACTCAG CCCTTTGGATATGAGCAAGATAATGATCACAAACCATCACATTTATATTCCTGCAACCACCGCTCCTTTG GATTCAAAACTACCTTCTCCAGATGCAAGGCCTACACAGTTTGGAATTCAAACAGCCACCTCTGAGTGGG TTTCCGATAAGTCTTTTGAAGGAAGAAAAAGAGAAGAAGATGAAGAGGGAGCTGTGAATGCAGCCCACCA AGGAGAAGTTCGAGCAGCAACAGAGAGGTCAGATCACTTACTTTTAACACCAGAATTAGAAAGTTCAAAT GTAGATGCATCTAGTGATTTGGCTACCTGGGAAGGTTTTATTCTAGAGACAACACCCACAGAGTCTGAAA AGGAAATGGCAAATTCAACTCCTGTTTTTAGAGAAACAATTGGTGTAGCCAATGTGGAGGCACAGCCCTT TGAGCATAGCAGTAGTAGTCATCCCAGGGTTCAGGAAGAGCTGACCACTCTCTCAGGAAATCCCCCCTCT CTTTTTACGGACCTGGGCTCAGGAGATGCTAGTACTGGTATGGAACTCATCACTGCTTCTTTATTTACAT TAGATTTAGAGTCTGAAACCAAAGTCAAAAAGGAATTACCCAGTACTCCGTCTCCCAGTGTGGAGATATC ATCCTCTTTTGAGCCAACAGGATTAACCCCGAGTACTGTACTAGACATAGAAATTGCTGGAGTTATGAGC CAGACATCGCAGAAGACACTGATTTCTGAAATATCAGGAAAGCCAACTTCCCAGTCAGGAGTAAGGGATT TGTATACAGGCTTTCCAATGGGAGAAGATTTTAGTGGTGACTTCAGTGAATATCCAACAGTGTCTTATCC AACGATGAAAGAGGAAACAGTTGGGATGGGAGGTTCTGACGATGAACGAGTTAGGGATACACAGACTTCA TCATCTATACCGACTACCTCAGACAACATCTACCCCGTGCCTGACTCAAAAGGACCTGACAGTACTGTGG CCAGCACCACAGCCTTCCCCTGGGAAGAGGTCATGTCCTCAGCTGAGGGGTCAGGTGAGCAATTAGCCTC AGTCAGGAGCTCTGTTGGTCCAGTGCTTCCACTAGCTGTGGACATTTTTTCTGGAACAGAGTCTCCATAT TTTGATGAAGAGTTTGAAGAAGTGGCTGCAGTGACAGAAGCAAATGAAAGACCCACTGTTTTGCCAACAG CAGCAAGTGGAAATACTGTAGATCTTACAGAAAATGGGTACATAGAGGTAAATAGCACGATGTCACTGGA CTTTCCCCAGACAATGGAGCCATCCAAATTGTGGTCTAAACCAGAAGTCAATCTTGATAAACAAGAGATT GGAAGAGAAACAGTGACAAAGGAAAAAGCTCAGGGACAAAAGACTTTTGAATCGCTCCACAGTTCTTTTG CACCAGAACAAACCATTTTGGAAACCCAATCACTTATTGAAACTGAATTCCAAACTTCCGATTACTCTAT GTTAACAACACTGAAAACGTACATTACTAATAAGGAAGTGGAGGAGGAAGGTATGTCCATAGCGCATATG TCTACTCCAGGTCCAGGGATCAAAGACTTGGAATCATATACTACCCATCCCGAAGCTCCTGGAAAGTCAC ACTCTTTCTCAGCTACTGCTTTAGTGACTGAATCTGGAGCAGCTAGAAGTGTACTCATGGATTCCTCAAC TCAAGAGGAAGAAAGCATAAAACTCTTCCAAAAGGGTGTGAAACTAACAAATAAAGAGTCAAATGCTGAT CTCTCATTCTCTGGACTGGGATCAGGAGAGGCTTTGCCTCCTCTACCTACAACATCAGTGAATTTGACTG ACATGAAGCAGATCATTAGCACATTGTATGCTGAGACATCTCACATGGAAAGCTTAGGAACAAGCATCTT AGGTGATAAAATGGAAGACCATGAAAGAATGGAAGATGTGTCCAATGAAGTTAGAATGCTCATTTCCAAA ACAGGCAGCATCTCTCAAGACAGCACAGAGGCACTCGACACAACTTTGAGTCATACCGGAACAGAAGAAC CTACAACTTCAACTCTTCCTTTCGTGAAGCTCATGGACTTGGAACGTTCTCCAAAGCAGACCCTCAGGTG GGAAGAGGAAACCCAGACCCATAGACCACAAACTATGAGTGGACTGATCTCTAATGAAAATTCTTCAGCA TCAGAAGCTGAAGAAGCAGCAACTTCCCCCACTGCTTTTCTGCCTCAAACTTACAGTGTTGAAATGACCA AACACTTTGCTCCATCAGAGTCACAACCATCCGACTTGTTTAATGTAAACTCTGGAGAGGGATCTGGAGA AGTGGATACTCTTGACTTAGTGTACACTTCTGGAACTACTCAGGCAAGCAGTCAAGGAGACAGCATGCTT GCTTCTCATGGATTTCTGGAAAAACATCCAGAGGTGTCAAAGACTGAGGCTGGAGCTACTGATGTATCTC CAACAGCCTCAGCAATGTTCCTGCATCACTCAGAGTACAAGAGTTCTCTGTATCCAACCAGCACACTGCC AAGCACAGAGCCATATAAAAGTCCCTCAGAAGGTATTGAAGATGGTTTGCAAGACAATATCCAATTTGAA GGTTCTACCTTGAAACCTAGCAGAAGAAAAACCACTGAAAGCATTATTATAGATCTAGACAAAGAGGATA GTAAGGACCTAGGATTGACAATTACTGAGAGTGCTATTGTTGAAATTCTCCCTGAGCTGACATCAGATAA AAATATTATTATAGACATTGATCACACTAAACCTGTATATGAGTACATCCCTGGAATACAAACAGACCTA GATCCAGAGATAAAATTGGAATCACATGGCAGTAGTGAAGAAAGCCTTCAAGTTCAAGAGAAGTATGAGG GAGCTGTTACCCTTTCTCCAACTGAGGAATCATTTGAAGGCTCTGGTGATGCGCTTCTTGCTGGCTATAC TCAAGCAATATACAATGAGTCTGTGACTCCCAACGATGGAAAGCAGGCAGAGGACATAAGCTTTAGTTTT GCCACTGGGATCCCAGTTTCTAGCACAGAGACAGAATTACACACTTTCTTCCCCACAGCGTCAACCCTGC ACATTCCTAGTAAACTAACCACAGCTAGTCCGGAGATTGATAAACCCAACATTGAAGCAATATCTCTGGA TGATATATTTGAATCAAGCACCTTGTCTGATGGCCAAGCTATTGCAGACCAGAGTGAAGTAATATCAACA TTAGGGCATTTGGAAAAAACACAGGAAGAATATGAAGAAAAGAAATATGGAGGTCCTTCATTTCAGCCAG AATTCTTTTCAGGAGTGGGGGAAGTATTGACAGATCCCCCTGCTTATGTAAGTATTGGTAGTACATACCT TATAGCTCAGACTTTAACAGAATTACCCAATGTGGTAAGACCATCTGACTCCACACATTACACTGAAGCA ACACCTGAAGTTTCCTCTCTGGCAGAGCTCTCTCCACAGATACCATCTTCTCCCTTCCCTGTCTACGTAG ACAATGGAGTCTCTAAATTCCCAGAGGTTCCCCATACAAGTGCTCAGCCAGTTTCTACTGTCACCTCATC ACAAAAGTCTATAGAGAGTCCTTTTAAGGAAGTCCATGCTAATATTGAAGAAACCATCAAGCCATTGGGT GGAAATGTCCACAGAACAGAGCCTCCATCCATGTCTCGTGACCCTGCTTTAGATGTTTCAGAAGATGAAA GTAAGCATAAGTTACTAGAAGAACTGGAAACGTCTCCCACCAAACCTGAGACTTCCCAAGACTTCCCAAA CAAAGCAAAGGATCACATTCCAGGAGAAACAGTTGGGATGCTTGCTGGTATCAGAACAACAGAGAGCGAA CCTGTCATTACTGCAGACGACATGGAGCTAGGAGGTGCCACACAGCAGCCACATTCTGCTTCTGCAGCTT TCAGAGTTGAGACAGGTATGGTACCTCAGCCCATCCAACAGGAGCCTGAGAGGCCAACCTTTCCTTCCTT GGAAATTAACCATGAAACACACACATCATTATTTGGAGAATCTATACTGGCAACATCTGAAAAACAAGTG TCTCAAAAAATTCTTGATAATAGTAACCAGGCAACAGTCAGTAGTACTCTGGATCTACATACTGCACATG CATTATCACCATTTTCCATTCTGGACAATTCTAATGAAACTGCTTTCCTGATTGGCATTAGTGAAGAGTC CGTGGAAGGCACAGCAGTTTACCTACCAGGACCTGATCTCTGCAAAACAAACCCATGCCTCAACGGAGGC ACCTGTTATCCTACCGAGACTTCCTATGTGTGCACCTGTGCACCTGGATACAGCGGAGACCAGTGTGAAC TTGATTTTGATGAATGTCACTCTAACCCTTGTCGGAATGGTGCCACCTGTGTGGATGGTTTTAATACATT TAGATGTCTCTGTCTCCCAAGTTATGTTGGTGCACTCTGTGAACAAGACACTGAGACATGTGACTATGGC TGGCACAAATTCCAAGGACAGTGCTACAAGTACTTTGCTCATCGACGCACATGGGATGCTGCTGAAAGGG AGTGTCGCCTGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGT GGGCCATGATTACCAGTGGATCGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGC AGTGCACTGCAATATGAGAACTGGAGACCCAACCAGCCAGACAGTTTCTTTTCTGCAGGAGAAGACTGCG TTGTGATCATTTGGCATGAAAATGGTCAGTGGAATGACGTCCCCTGCAATTACCACCTCACCTACACTTG CAAGAAGGGAACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAA CCACGCTATGAAATCAACTCCTTGATTAGATATCACTGCAAAGATGGTTTCATTCAGCGACACCTTCCAA CTATCCGGTGCCTAGGAAACGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCA AAGGACTTATTCTAAGAAATACTTAAAAAATTCCTCATCCGCAAAGGACAATTCTATAAATACATCAAAA CATGAGCATCGCTGGAGCCGGAGGCGGCAGGAAACCAGGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225930 representing NM_019389 Red=Cloning site Green=Tags(s) MLINMKGILWMCSTLLLTHALHQAKMETSPPVKGSLSGKVVLPCHFSTLPTLPPNYNTSEFLRIKWSKME VDKNGKDIKETTVLVAQNGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQ DTMSLAVDGVVFHYRAATSRYTLNFAAAQQACLDIGAVIASPEQLFAAYEDGFEQCDAGWLSDQTVRYPI RAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECTSRDARLATV GELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKRRL SDMIVSGHPIDSESKEEEPCSEETDPLHDLFAEILPELPDSFEIDIYHSEEDEDGEEDCVNATDVTTTPS VQYINGKQLVTTVPKDPEAAEARRGQYESVAPSQNFPDSSATDTHQFILAETESSTTMQFKKSKEGTELL EITWKPETYPETPDHVSSGEPDVFPTLSSHDGKTTRWSESITESSPNLENPVHKQPKPVPLFPEESSGEG AIEQASQETILSRATEVALGKETDQSPTLSTSSILSSSVSVNVLEEEPLTLTGISQTDESMSTIESWVEI TPSQTVKFSESSSAPIIEGSGEVEENKNKIFNMVTDLPQRDPTDTLSPLDMSKIMITNHHIYIPATTAPL DSKLPSPDARPTQFGIQTATSEWVSDKSFEGRKREEDEEGAVNAAHQGEVRAATERSDHLLLTPELESSN VDASSDLATWEGFILETTPTESEKEMANSTPVFRETIGVANVEAQPFEHSSSSHPRVQEELTTLSGNPPS LFTDLGSGDASTGMELITASLFTLDLESETKVKKELPSTPSPSVEISSSFEPTGLTPSTVLDIEIAGVMS QTSQKTLISEISGKPTSQSGVRDLYTGFPMGEDFSGDFSEYPTVSYPTMKEETVGMGGSDDERVRDTQTS SSIPTTSDNIYPVPDSKGPDSTVASTTAFPWEEVMSSAEGSGEQLASVRSSVGPVLPLAVDIFSGTESPY FDEEFEEVAAVTEANERPTVLPTAASGNTVDLTENGYIEVNSTMSLDFPQTMEPSKLWSKPEVNLDKQEI GRETVTKEKAQGQKTFESLHSSFAPEQTILETQSLIETEFQTSDYSMLTTLKTYITNKEVEEEGMSIAHM STPGPGIKDLESYTTHPEAPGKSHSFSATALVTESGAARSVLMDSSTQEEESIKLFQKGVKLTNKESNAD LSFSGLGSGEALPPLPTTSVNLTDMKQIISTLYAETSHMESLGTSILGDKMEDHERMEDVSNEVRMLISK TGSISQDSTEALDTTLSHTGTEEPTTSTLPFVKLMDLERSPKQTLRWEEETQTHRPQTMSGLISNENSSA SEAEEAATSPTAFLPQTYSVEMTKHFAPSESQPSDLFNVNSGEGSGEVDTLDLVYTSGTTQASSQGDSML ASHGFLEKHPEVSKTEAGATDVSPTASAMFLHHSEYKSSLYPTSTLPSTEPYKSPSEGIEDGLQDNIQFE GSTLKPSRRKTTESIIIDLDKEDSKDLGLTITESAIVEILPELTSDKNIIIDIDHTKPVYEYIPGIQTDL DPEIKLESHGSSEESLQVQEKYEGAVTLSPTEESFEGSGDALLAGYTQAIYNESVTPNDGKQAEDISFSF ATGIPVSSTETELHTFFPTASTLHIPSKLTTASPEIDKPNIEAISLDDIFESSTLSDGQAIADQSEVIST LGHLEKTQEEYEEKKYGGPSFQPEFFSGVGEVLTDPPAYVSIGSTYLIAQTLTELPNVVRPSDSTHYTEA TPEVSSLAELSPQIPSSPFPVYVDNGVSKFPEVPHTSAQPVSTVTSSQKSIESPFKEVHANIEETIKPLG GNVHRTEPPSMSRDPALDVSEDESKHKLLEELETSPTKPETSQDFPNKAKDHIPGETVGMLAGIRTTESE PVITADDMELGGATQQPHSASAAFRVETGMVPQPIQQEPERPTFPSLEINHETHTSLFGESILATSEKQV SQKILDNSNQATVSSTLDLHTAHALSPFSILDNSNETAFLIGISEESVEGTAVYLPGPDLCKTNPCLNGG TCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYG WHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDG SALQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMK PRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSAKDNSINTSK HEHRWSRRRQETRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019389
ORF Size	7182 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019389.2, NP_062262.2
RefSeq Size	9552 bp
RefSeq ORF	7185 bp
Locus ID	13003
MW	263.2 kDa

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