United States
Germany
Japan
United Kingdom
China
Nedd4l (NM_001114386) Mouse Tagged ORF Clone
CAT＃: MR225817
- TrueORF®
Nedd4l (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 4-like (Nedd4l), transcript variant 1
ORF Plasmid: tGFP
"NM_001114386" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 9,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300012C07Rik; Nedd4-2; Nedd4b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225817 representing NM_001114386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGGGCTTGGGGAGCCTGTCTACGGACTTTCGGAAGAGGAGGGAGAATCCCGTATCCTCAGAG TAAAAGTTGTTTCTGGAATTGACCTCGCCAAGAAGGACATATTTGGAGCCAGCGACCCATACGTGAAGCT GTCCTTGTATGTAGCTGATGAGAATAGAGAACTTGCTCTGGTTCAAACCAAGACAATTAAAAAGACGCTG AACCCAAAGTGGAATGAAGAGTTTTATTTTAGGGTAAATCCATCTAATCACAGGCTCCTATTTGAAGTAT TTGACGAGAACAGATTGACACGAGACGACTTCCTGGGCCAGGTGGACGTGCCTCTTAGTCACCTTCCGAC AGAAGATCCAACCATGGAGAGACCCTATACATTTAAGGATTTTCTCCTGCGACCTAGAAGTCATAAATCT CGAGTCAAGGGGTTTTTGAGGTTGAAAATGGCCTACATGCCGAAAAACGGAGGTCAGGATGAAGAAAACA GCGAGCAGAGGGATGACATGGAGCATGGATGGGAAGTGGTTGACTCCAACGACTCAGCTTCCCAGCACCA GGAGGAGCTCCCCCCTCCTCCCCTGCCGCCAGGATGGGAAGAGAAAGTGGACAATTTAGGCCGAACTTAC TATGTCAACCACAACAACAGGAGTACTCAGTGGCACCGACCCAGCTTGATGGATGTGTCCTCGGAATCAG ACAATAACATCAGGCAGATCAACCAGGAGGCTGCACACCGACGCTTCCGCTCTCGGAGGCACATTAGTGA AGATTTGGAGCCTGAGGCCTCTGAAGGCGGTGGAGAAGGCCCTGAGCCTTGGGAGACCATTTCAGAGGAA ATGAACATGGCAGGAGATTCTCTCAGCCTGGCTCTGCCCCCACCGCCTGCCTCCCCAGTGTCCCGGACCA GCCCCCAGGAGCTGTCGGAAGAAGTGAGCCGAAGGTTGCAGATCACTCCGGACTCCAACGGGGAACAGTT CAGTTCTCTGATTCAGAGAGAGCCCTCGTCAAGGCTTCGGTCCTGCAGTGTTACCGACACGGTTGCTGAG CAAGCTCACCTTCCACCGCCCAGCACCCCAACTAGGCGAGCCCGTTCGTCAACTGTCACGGGTGGTGAGG AATCCACGCCATCAGTGGCCTATGTACATACCACGCCGGGCCTGCCTTCAGGCTGGGAAGAAAGAAAGGA TGCAAAGGGACGCACATACTATGTCAATCATAACAATCGAACCACAACTTGGACTCGGCCAATCATGCAG CTTGCAGAAGACGGTGCCTCCGGATCAGCCACAAACAGTAACAACCACCTAGTCGAACCCCAGATCCGCC GGCCCCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCACTGGAGGGTGCCAAGGATTCACCCAT CCGCCGTGCCGTGAAAGATACCCTTTCCAATCCACAGTCCCCTCAGCCATCACCTTACAACTCCCCCAAA CCACAACACAAAGTCACACAGAGCTTCCTGCCACCAGGCTGGGAGATGAGGATAGCCCCCAACGGCCGAC CCTTCTTCATTGACCATAACACAAAGACTACAACCTGGGAAGATCCACGGTTGAAATTTCCAGTACACAT GCGGTCAAAAGCATCTTTAAACCCCAATGACCTGGGCCCTCTTCCTCCTGGCTGGGAAGAGAGGATCCAC TTGGATGGCCGCACGTTTTACATTGACCATAATAGTAAAATTACCCAGTGGGAAGATCCAAGACTACAGA ACCCAGCCATCACTGGTCCGGCTGTTCCGTACTCCAGAGAGTTTAAGCAGAAATACGACTACTTTAGGAA GAAATTAAAGAAGCCTGCTGATATTCCAAACAGGTTTGAAATGAAACTTCACAGAAACAACATATTTGAA GAGTCCTATCGGAGGATCATGTCTGTAAAGAGACCTGACGTCCTAAAGGCTAGGCTGTGGATTGAGTTTG AATCAGAGAAAGGCCTGGACTATGGGGGCGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTT TAACCCCTACTATGGCCTCTTCGAGTACTCTGCCACGGACAACTACACACTTCAGATCAATCCCAACTCA GGCCTCTGTAATGAAGACCATTTGTCCTATTTCACCTTCATTGGAAGAGTTGCTGGCCTAGCGGTGTTTC ATGGGAAACTCTTAGATGGATTCTTCATTCGACCATTCTACAAGATGATGCTGGGGAAGCAGATAACGCT GAACGACATGGAGTCCGTGGACAGCGAGTACTACAACTCTTTGAAGTGGATCTTAGAAAACGACCCCACG GAACTTGACCTCATGTTCTGCATAGACGAAGAGAACTTTGGGCAGACATACCAAGTGGATCTGAAGCCCA ACGGGTCAGAAATAATGGTAACCAATGAGAACAAACGAGAATACATTGACTTAGTCATCCAGTGGAGATT TGTGAACAGGGTCCAGAAGCAAATGAATGCCTTCTTGGAGGGATTTACAGAACTTCTTCCAATCGACTTG ATTAAAATTTTTGATGAAAATGAGCTGGAGTTGCTGATGTGCGGCCTTGGTGATGTCGACGTGAACGACT GGAGACAGCACTCTATTTACAAGAACGGCTACTGCCCCAACCACCCTGTCATCCAGTGGTTCTGGAAGGC CGTGCTCCTGATGGATGCTGAGAAGCGCATCCGGTTACTACAGTTTGTCACAGGCACCTCCAGAGTACCC ATGAATGGATTTGCCGAACTCTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTC CCGAAAAACTACCCAGAGCTCATACATGCTTTAATCGCCTTGATTTACCTCCATATGAAACCTTTGAAGA TTTACGGGAGAAACTTCTCATGGCTGTGGAAAACGCTCAAGGCTTCGAAGGTGTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225817 representing NM_001114386
Red=Cloning site Green=Tags(s) MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTL NPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKS RVKGFLRLKMAYMPKNGGQDEENSEQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTY YVNHNNRSTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWETISEE MNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDTVAE QAHLPPPSTPTRRARSSTVTGGEESTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQ LAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPK PQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIH LDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFE ESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNS GLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPT ELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDL IKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVP MNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114386 |
| ORF Size | 2928 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114386.1, NP_001107858.1 |
| RefSeq Size | 8163 bp |
| RefSeq ORF | 2931 bp |
| Locus ID | 83814 |
| MW | 112.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225817 | Nedd4l (tGFP-tagged) - Mouse neural precursor cell expressed developmentally down-regulated gene 4-like (Nedd4l) transcript variant 1, (10ug) |
CNY 10,640.00 |
|
| MR225817L3 | Lenti ORF clone of Nedd4l (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 4-like (Nedd4l), transcript variant 1 |
CNY 11,590.00 |
|
| MR225817L4 | Lenti ORF clone of Nedd4l (mGFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 4-like (Nedd4l), transcript variant 1 |
CNY 11,590.00 |
