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Mcoln2 (NM_026656) Mouse Tagged ORF Clone
CAT＃: MR225267
- TrueORF®
Mcoln2 (Myc-DDK-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 1
ORF Plasmid: tGFP
"NM_026656" in other vectors (3)
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CNY 6,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3300002C04Rik; AI549968; C86638; TRPML2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225267 representing NM_026656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGAGACGAGGAGACGCTGGACCTGCCTGCGTGGAACCGCGTCCCGGACCTGACTTGGGGTCCCC ACCACAGATCTGCGATGGCGTCTCTCGATTCTGAGGTGAGGGAAGAGTGTCTGAGGGAAGACCTGAAGTT CTACTTCATGAGTCCCTGTGAGAAGTACCGAGCCAGACGTCAGATCCCGTGGAAACTGGGCTTGCAGATA TTGAAGATAGTCATGGTAACCACACAGCTTGTTCGGTTTGGCTTAAGTAACCAGTTGGTGGTGGCTTTCA AAGAAGATAACACTGTTGCTTTTAAGCACTTGTTTTTGAAAGGATTTTCTGGAGTTGATGAAGATGACTA CAGCTGTAGCATATACACTCAGGAGAACACCTATGAGAGCATCTTTTTTGCTATAAAGCAGTATCGTCAT CTGAAGAATATTTCCCTGGCAACCCTTGGTTATGGAGAAAGTGAAGACAATAGAACTGGCTTGAAGGTCT GTAAGCAGCACTACAAGACAGGGGCCATGTTTTCTTCTAACGAGACCCTGAACATTGACAGTGACATTGA GACAGACTGCATTCACCTGGACCTTCAGGTGCTCACCACGGAACCCGAGGACTGGGCACAGACATCTTTC TTCAGGCTGGATTTTTATCGGCTCGTGCAAGTTGATATCTCCTTTGCCCTCAAAGGAATTGACCTACAGG CAGTTCATTCCCGAGAGATACCGGACTGTTACCTCTTTCAGAATACGATTACCTTCGACAACACAGCCCA CAGTGGGAAAATCAAAATCTATCTCAACAGCGAGGCCAACATCGAAGAGTGTAAAAACATGAACATATCT GGGTCTACTCAGAGAAGCACTCACTACCTCCTGGTGTTTGATGTGTTCGTCATTATGATCTGCCTGGCAT CCCTTATCCTGTGCACGAGATCCATTGTTCTTGCTCTGAGGCTGAGGAAGAGATTTCTAAACTTCTTCCT GGAGAAGTACAAACAGCGCGTGTGTGGCGCTGACCAGTGGGAATTCGTCAACGGATGGTACGTCCTGGTC ACTATCAGCGACCTCATGACAATAATTGGATCCATATTGAAGATGGAAATCAAAGCCAAGAAGCTCACAA ACTATGACGTGTGCAGCATCTTGCTCGGGACGTCGACGCTCTTTGTCTGGGTTGGAGTCATCAGATATCT GGGTTACTTTCAGACGTACAACGTGCTCATTTTAACCATGCAAGCCTCACTGCCCAAAGTTCTCCGGTTC TGTGCTTGCGCTGGCATGATCTACCTGGGGTACACCTTCTGCGGCTGGATTGTCTTAGGACCGTACCACG AGAAGTTTGAAAACCTGAACATAGTCGCTGAATGCCTGTTTTCTCTGGTCAACGGCGATGACATGTTTGC CACGTTCGCTCAGATCCAGCAGAAGAGCATTCTGGTGTGGCTGTTCAGCCGCCTCTACCTGTATTCCTTT ATCAGCCTATTCATTTACATGGTCCTCAGCCTCTTCATCGCGCTCATCACCGACTCTTACCACACCATTA AGAAGTACCAGCAGCATGGGTTTCCTGAAACAGATCTGCAGAAATTCCTGAAGGAGAGCGGAAGCAAAGA TGGGTACCAGAAGCAGCCATCGGCCTTGCTGTCCTGCCTCTGCTGTCTGAGGAGGAGAAGAAGCAATGAC CACTTGATTCTCATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225267 representing NM_026656
Red=Cloning site Green=Tags(s) MPGDEETLDLPAWNRVPDLTWGPHHRSAMASLDSEVREECLREDLKFYFMSPCEKYRARRQIPWKLGLQI LKIVMVTTQLVRFGLSNQLVVAFKEDNTVAFKHLFLKGFSGVDEDDYSCSIYTQENTYESIFFAIKQYRH LKNISLATLGYGESEDNRTGLKVCKQHYKTGAMFSSNETLNIDSDIETDCIHLDLQVLTTEPEDWAQTSF FRLDFYRLVQVDISFALKGIDLQAVHSREIPDCYLFQNTITFDNTAHSGKIKIYLNSEANIEECKNMNIS GSTQRSTHYLLVFDVFVIMICLASLILCTRSIVLALRLRKRFLNFFLEKYKQRVCGADQWEFVNGWYVLV TISDLMTIIGSILKMEIKAKKLTNYDVCSILLGTSTLFVWVGVIRYLGYFQTYNVLILTMQASLPKVLRF CACAGMIYLGYTFCGWIVLGPYHEKFENLNIVAECLFSLVNGDDMFATFAQIQQKSILVWLFSRLYLYSF ISLFIYMVLSLFIALITDSYHTIKKYQQHGFPETDLQKFLKESGSKDGYQKQPSALLSCLCCLRRRRSND HLILID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026656 |
| ORF Size | 1698 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026656.5 |
| RefSeq Size | 2519 bp |
| RefSeq ORF | 1701 bp |
| Locus ID | 68279 |
| UniProt ID | Q8K595 |
| MW | 65.9 kDa |
| Gene Summary | Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19763610). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (By similarity). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (PubMed:17050035). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (PubMed:26432893). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225267 | Mcoln2 (tGFP-tagged) - Mouse mucolipin 2 (Mcoln2) transcript variant 1, (10ug) |
CNY 6,180.00 |
|
| MR225267L3 | Lenti ORF clone of Mcoln2 (Myc-DDK-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 1 |
CNY 7,510.00 |
|
| MR225267L4 | Lenti ORF clone of Mcoln2 (mGFP-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 1 |
CNY 7,510.00 |
