United States
Germany
Japan
United Kingdom
China
Chrm3 (NM_033269) Mouse Tagged ORF Clone
CAT＃: MR225258
- TrueORF®
Chrm3 (Myc-DDK-tagged) - Mouse cholinergic receptor, muscarinic 3, cardiac (Chrm3)
ORF Plasmid: tGFP
"NM_033269" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,592.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Chrm-3; M3; M3R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGACCTTGCACAGTAACAGTACAACCTCGCCTTTGTTTCCCAACATCAGCTCTTCCTGGGTGCACAGTC CCTCAGAGGCGGGGCTGCCCTTGGGGACAGTCTCTCAATTGGACAGCTACAACATTTCCCAAACCTCTGG GAATTTCTCCTCAAATGACACCTCCAGTGACCCTCTAGGGGGCCACACCATCTGGCAAGTGGTCTTCATT GCATTCTTGACTGGCTTCCTGGCATTGGTGACCATCATCGGCAACATCCTTGTCATTGTGGCATTTAAGG TCAACAAACAGCTGAAGACAGTCAACAACTACTTCCTCTTAAGCCTGGCCTGCGCAGATCTGATCATCGG GGTCATTTCCATGAACCTGTTCACGACCTACATCATTATGAACCGCTGGGCTCTGGGGAACTTAGCCTGT GACCTCTGGCTTTCCATTGACTATGTGGCCAGCAATGCTTCTGTCATGAATCTGCTGGTGATCAGCTTTG ACAGGTACTTTTCTATTACCAGGCCACTCACTTACCGAGCCAAACGAACAACAAAACGAGCCGGTGTGAT GATTGGTCTGGCTTGGGTCATCTCCTTTGTCCTGTGGGCTCCTGCCATCTTGTTCTGGCAATACTTTGTA GGGAAGAGAACTGTGCCCCCCGGAGAATGTTTCATTCAGTTTCTAAGTGAGCCCACCATCACCTTCGGCA CGGCGATCGCTGCCTTTTACATGCCTGTCACCATCATGACTATTTTATACTGGAGAATCTATAAGGAGAC TGAGAAACGTACCAAAGAGCTGGCTGGGCTACAGGCCTCTGGGACAGAAGCGGAAGCAGAAAACTTTGTC CACCCCACAGGCAGTTCTCGAAGCTGTAGCAGCTATGAGCTACAACAGCAAGGCACGAAACGGTCATCTA GGAGGAAGTATGGTGGCTGTCACTTCTGGTTCACAACTAAGAGCTGGAAGCCCAGTGCTGAGCAGATGGA CCAAGACCACAGTAGCAGTGACAGTTGGAATAACAACGATGCTGCTGCCTCCCTGGAAAACTCTGCTTCT TCTGATGAAGAGGATATTGGCTCAGAGACCAGAGCCATCTATTCCATTGTACTCAAGCTGCCGGGTCATA GCACCATCCTCAACTCTACCAAGCTACCCTCCTCAGATAACCTGCAGGTGCCAGACAAGGACCTGGGGAC TATGGATGTAGAGAGAAATGCCCATAAGCTTCAGGCCCAGAAGAGTATGGATGACCGTGACAACTGTCAG AAGGACTTCTCCAAGCTCCCCATCCAGTTAGAGTCTGCCGTGGACACAGCCAAGACCTCTGACACCAACT CCTCGGTGGACAAGACCACGGCCGCTCTACCTCTGTCCTTCAAAGAAGCCACGCTGGCTAAGAGGTTTGC TCTCAAGACCAGAAGTCAGATCACCAAGCGGAAAAGGATGTCGCTCATCAAGGAGAAGAAGGCCGCCCAG ACACTCAGTGCCATCTTGCTGGCTTTCATCATCACGTGGACCCCCTACAACATCATGGTCCTGGTGAACA CCTTCTGTGACAGCTGCATACCCAAAACCTATTGGAATCTGGGCTACTGGCTGTGCTATATCAACAGCAC CGTGAACCCCGTGTGCTATGCCCTGTGCAACAAGACATTCAGAACCACCTTCAAGATGCTTCTCTTATGC CAGTGTGACAAGAGGAAGCGGCGCAAACAGCAGTACCAGCAGAGACAGTCCGTCATTTTTCACAAGCGAG TGCCTGAGCAGGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225258 protein sequence
Red=Cloning site Green=Tags(s) MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVSQLDSYNISQTSGNFSSNDTSSDPLGGHTIWQVVFI AFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLAC DLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFV GKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFV HPTGSSRSCSSYELQQQGTKRSSRRKYGGCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSAS SDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPDKDLGTMDVERNAHKLQAQKSMDDRDNCQ KDFSKLPIQLESAVDTAKTSDTNSSVDKTTAALPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQ TLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLLLC QCDKRKRRKQQYQQRQSVIFHKRVPEQAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033269 |
| ORF Size | 1770 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033269.4, NP_150372.1 |
| RefSeq Size | 3168 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 12671 |
| UniProt ID | Q9ERZ3 |
| MW | 66.2 kDa |
| Gene Summary | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219378 | Chrm3 (untagged) - Mouse cholinergic receptor, muscarinic 3, cardiac (Chrm3), (10ug) |
CNY 7,510.00 |
|
| MG225258 | Chrm3 (tGFP-tagged) - Mouse cholinergic receptor muscarinic 3 cardiac (Chrm3), (10ug) |
CNY 6,460.00 |
|
| MR225258L3 | Lenti ORF clone of Chrm3 (Myc-DDK-tagged) - Mouse cholinergic receptor, muscarinic 3, cardiac (Chrm3) |
CNY 7,790.00 |
|
| MR225258L4 | Lenti ORF clone of Chrm3 (mGFP-tagged) - Mouse cholinergic receptor, muscarinic 3, cardiac (Chrm3) |
CNY 7,790.00 |
