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Ehmt2 (NM_147151) Mouse Tagged ORF Clone
CAT＃: MR222625
- TrueORF®
Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short
ORF Plasmid: tGFP
"NM_147151" in other vectors (4)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bat8; D17Ertd710e; G9a; KMT1C; NG36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222625 representing NM_147151
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGAGCTGCTGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGC TGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAGTTCATAGCTCTTTGGGGGACACCCCTCAGAG TGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGAGCCTGCCGGCCCCTCCTCTCCGGCCTCTGTC ACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTCGGGGCCGCATCACTCATCGGGGACGAACCCG AGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCCATGCCACAAAGTCGTTCCCCTCTTCCCCCAG CAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAATGACAGGGGCAGGAAAGTCGCCCCCCTCGGTC CAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCCCAGGGAGCTGCAACTGCTGGGCCTGAACCCT CTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAGTGCACCGAGCCCGGAAAACCATGTCCAAACC TAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCCTGAAGTCCAGCATTTCCGCATGAGTGATGAC ATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGGAGGAAGCTGAACTCTGGTAGCCTGTCCGAGG ACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGGAGAAGGGAGAGCCCAGGCCCCTGGAGGAGTG GGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGATGCGTACTCTGTGGATGAGCGGGTGGACTCT GACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTGAGTGAGGAGGAGGAGGAGGAAGAGGAGGAAG AAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGAGGACGAGGAGTCGGGCAATCAGTC AGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGGAAAGACAGCCCGTGGGTGAAG CCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCAAGAGGGGTCTCCAATGACACGT CTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCTCCCAAGAT TGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGTGTGGATGGAGAGCTCCTGGGC TGCAATGCTGCCATCCTTAAGCGGGAGACCATGCGGCCGTCTAGCCGCGTGGCGCTGATGGTGCTCTGTG AGGCCCATCGAGCCCGCATGGTCAAGCACCATTGCTGCCCGGGCTGCGGCTACTTCTGCACAGCGGGCAC CTTCCTGGAATGCCACCCCGACTTTCGTGTAGCTCACCGCTTCCATAAGGCCTGCGTATCCCAGCTCAAT GGGATGGTCTTCTGTCCCCACTGTGGAGAGGATGCCTCAGAGGCCCAGGAGGTGACCATTCCTCGGGGCG ATGGGGGAACACCCCCAATTGGCACCGCAGCTCCTGCTCTGCCACCCCTGGCACATGATGCCCCAGGGCG AGCGGATACCTCCCAGCCTAGCGCCCGAATGCGAGGGCATGGAGAGCCGCGGCGCCCGCCCTGTGATCCC CTGGCTGACACCATCGACAGCTCAGGGCCTTCACTGACTCTGCCTAATGGGGGCTGCCTCTCCGCTGTGG GTCTGCCCCCAGGGCCGGGCAGGGAAGCCCTGGAAAAAGCCTTGGTCATCCAGGAGTCTGAGAGGCGGAA GAAGCTGCGATTCCACCCACGGCAGCTGTACCTGTCGGTGAAGCAGGGGGAGCTGCAGAAGGTGATCCTT ATGCTGTTAGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCACGCGG CCGCCCAGAAGGGGTCGGTAGAGATCTGTCATGTGCTGCTGCAGGCAGGAGCCAACATCAATGCCGTAGA TAAGCAACAACGCACGCCACTAATGGAGGCCGTGGTGAACAACCACCTGGAGGTGGCACGCTACATGGTG CAGTTAGGTGGCTGTGTCTACAGCAAGGAAGAGGATGGCTCCACCTGTCTACATCATGCAGCCAAAATTG GGAACTTGGAAATGGTCAGCCTGCTACTGAGCACAGGACAGGTGGACGTCAATGCCCAGGACAGTGGGGG CTGGACGCCCATCATCTGGGCAGCCGAGCACAAGCACATCGATGTGATTCGTATGCTGCTGACCCGGGGT GCCGATGTCACCCTGACTGACAATGAGGAAAACATCTGCCTGCACTGGGCCTCCTTCACGGGTAGTGCCG CCATCGCTGAGGTCCTTCTGAATGCCCAGTGTGATCTCCATGCTGTCAACTACCATGGGGACACGCCCCT GCACATAGCCGCCAGGGAGAGCTACCATGACTGTGTTCTGTTGTTCCTGTCTCGTGGAGCCAACCCTGAG CTTCGGAACAAAGAAGGAGACACGGCATGGGATCTGACCCCAGAGCGCTCTGATGTGTGGTTTGCACTGC AGCTCAATCGAAAGCTTAGGCTTGGGGTAGGGAACCGGGCTGTCCGCACCGAGAAGATCATCTGCCGGGA CGTAGCCCGAGGCTATGAGAATGTACCCATCCCCTGTGTCAATGGTGTGGATGGGGAGCCGTGCCCGGAG GACTACAAGTACATCTCTGAGAACTGCGAGACATCGACCATGAACATCGACCGCAACATCACCCATCTGC AGCACTGCACGTGTGTGGATGACTGCTCCAGCTCCAATTGCCTATGTGGTCAGCTCAGTATCCGATGCTG GTATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACAAGATCGAGCCCCCCCTGATCTTTGAGTGTAAC CAGGCATGCTCCTGCTGGAGAAGCTGCAAGAACCGCGTGGTGCAGAGCGGCATCAAGGTACGGCTGCAGC TCTACCGGACTGCCAAGATGGGCTGGGGGGTCCGAGCCTTGCAGACCATCCCCCAGGGCACGTTCATCTG CGAGTATGTAGGAGAGCTGATCTCTGATGCCGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGAT TTAGATAACAAGGATGGCGAGGTTTACTGCATTGATGCCCGTTACTATGGCAACATCAGCCGATTCATTA ACCACCTGTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTGCACCAAGATCTACGGTTCCCACG CATTGCCTTCTTCAGCTCCAGGGACATCCGGACTGGGGAGGAGCTGGGCTTTGACTACGGTGACCGATTC TGGGACATCAAGAGCAAGTATTTCACCTGCCAGTGTGGCTCTGAGAAGTGCAAGCATTCAGCGGAGGCCA TCGCCCTGGAGCAGAGCCGCCTGGCCCGGCTGGACCCCCACCCGGAGCTGCTCCCTGACCTCAGCTCCCT GCCCCCCATCAACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222625 representing NM_147151
Red=Cloning site Green=Tags(s) MAAAAGAAAAAAAEGEAPAEMGALLLEKEPRGAAERVHSSLGDTPQSEETLPKANPDSLEPAGPSSPASV TVTVGDEGADTPVGAASLIGDEPESLEGDGGRIVLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSV QSLAMRLLSMPGAQGAATAGPEPSPATTAAQEGQPKVHRARKTMSKPSNGQPPIPEKRPPEVQHFRMSDD MHLGKVTSDVAKRRKLNSGSLSEDLGSAGGSGDIILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDS DSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVK PSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLG CNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLN GMVFCPHCGEDASEAQEVTIPRGDGGTPPIGTAAPALPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDP LADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVIL MLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMV QLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRG ADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPE LRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPE DYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECN QACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFD LDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRF WDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147151 |
| ORF Size | 3516 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_147151.3 |
| RefSeq Size | 3757 bp |
| RefSeq ORF | 3519 bp |
| Locus ID | 110147 |
| UniProt ID | Q9Z148 |
| MW | 128.9 kDa |
| Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223836 | Ehmt2 (untagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short, (10ug) |
CNY 15,110.00 |
|
| MG222625 | Ehmt2 (tGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2) transcript variant short, (10ug) |
CNY 12,830.00 |
|
| MR222625L3 | Lenti ORF clone of Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short |
CNY 13,590.00 |
|
| MR222625L4 | Lenti ORF clone of Ehmt2 (mGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short |
CNY 13,590.00 |
