Myo5a (NM_010864) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9630007J19Rik; AI413174; AI661011; d; d-120J; Dbv; flail; flr; MVa; Myo5; MyoVA; Sev-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221640 representing NM_010864 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGTCCGAGCTCTACACCAAGTTTGCCAGGGTTTGGATCCCTGATCCTGAGGAAGTGTGGAAAT CGGCAGAGTTGCTCAAGGATTATAAGCCTGGAGATAAAGTGCTCCTGCTTCACCTCGAGGAAGGGAAGGA TTTGGAATACCGTCTAGACCCAAAGACCGGTGAGCTCCCTCACTTACGGAACCCTGACATACTTGTTGGA GAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCCGCTGTGCTACATAATCTCCGAGTTCGCTTCA TCGATTCCAAACTTATTTATACGTATTGTGGAATAGTTCTGGTAGCTATAAATCCCTATGAGCAGCTGCC TATTTATGGAGAAGATATTATTAATGCCTACAGTGGCCAGAACATGGGTGACATGGATCCTCACATCTTC GCAGTAGCTGAAGAGGCTTACAAGCAAATGGCAAGGGATGAACGAAATCAGTCCATCATTGTAAGTGGAG AGTCAGGCGCAGGGAAGACGGTCTCTGCTAAGTATGCCATGCGGTACTTCGCAACTGTAAGTGGCTCTGC CAGTGAGGCCAATGTTGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCAATTGGAAATGCCAAA ACAACCAGGAATGATAATAGCAGCCGGTTTGGAAAATATATTGAAATTGGTTTTGACAAGAGGTACAGAA TCATCGGTGCCAATATGAGAACTTACCTTTTAGAGAAATCCAGAGTGGTGTTCCAGGCAGAAGAGGAGAG AAACTACCATATCTTCTATCAGCTCTGTGCTTCGGCAAAGTTACCTGAGTTTAAAATGCTGCGGTTAGGA AATGCAGATAGTTTTCATTACACGAAGCAAGGAGGCAGCCCTATGATAGAAGGAGTAGATGATGCGAAGG AGATGGCGCACACCAGGCAGGCCTGCACTCTGCTGGGAATTAGTGAATCTTACCAAATGGGAATTTTTCG AATACTTGCTGGCATTCTTCACTTAGGCAATGTTGGATTTGCATCTCGGGATTCAGACAGCTGCACAATA CCTCCCAAGCACGAACCTCTTACCATCTTCTGTGACCTTATGGGTGTGGATTATGAAGAGATGTGTCACT GGCTCTGCCACCGAAAGCTGGCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCAC AAATGCCCGAGATGCTTTAGCAAAGCACATCTATGCAAAGCTCTTTAACTGGATTGTTGACCACGTCAAT CAGGCTCTCCATTCTGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATCTATGGATTTGAAACAT TTGAAATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAACTACAACAACAATTCAACAT GCATGTCTTCAAATTGGAACAGGAGGAATATATGAAGGAACAGATTCCATGGACACTTATAGATTTCTAT GATAATCAGCCTTGTATCAATCTTATAGAATCTAAACTGGGAATTCTCGATTTGCTGGATGAGGAATGTA AGATGCCTAAAGGCACAGATGACACATGGGCCCAAAAACTGTACAACACACATTTGAACAAATGTGCCCT CTTTGAGAAGCCCCGCATGTCAAACAAAGCTTTCATCATCAAACATTTTGCTGACAAAGTGGAGTACCAG TGTGAAGGTTTTCTTGAAAAGAATAAAGATACTGTTTTTGAAGAACAAATTAAAGTCCTTAAGTCAAGCA AGTTTAAGATGCTACCAGAGCTATTTCAAGATGATGAGAAGGCCATCAGTCCCACCTCTGCCACCTCCTC AGGGCGCACTCCTCTCACTCGAGTTCCTGTAAAGCCCACCAAGGGCCGACCTGGCCAGACTGCCAAAGAG CACAAGAAGACAGTGGGACATCAGTTTCGAAACTCCCTTCACCTGCTTATGGAAACCCTTAATGCCACTA CTCCTCACTATGTACGCTGTATTAAGCCTAATGATTTCAAGTTTCCATTCACATTTGATGAGAAGAGGGC AGTGCAGCAGCTAAGAGCATGTGGTGTCCTGGAGACCATCCGGATCAGCGCAGCAGGGTTTCCCTCACGG TGGACTTACCAAGAGTTTTTCAGCCGGTACCGGGTCCTAATGAAGCAAAAAGATGTGCTGGGAGATAGAA AGCAAACGTGCAAGAATGTATTAGAGAAACTAATATTGGACAAGGATAAATACCAGTTTGGTAAGACAAA GATCTTTTTCCGTGCTGGTCAAGTGGCCTATCTTGAAAAATTGAGGGCTGACAAACTTCGGGCTGCCTGC ATCCGGATCCAGAAGACCATTCGTGGGTGGCTTCTAAGGAAGAGATACCTGTGTATGCAGAGGGCAGCCA TCACAGTGCAGCGATACGTGCGGGGCTATCAGGCTCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC AGCAACCACCATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC ACGATTGTTATTCAGTCTTACTTGAGAGGCTACTTGACAAGAAATAGGTATCGCAAGATACTCCGTGAAT ACAAAGCAGTCATCATTCAGAAACGTGTCCGTGGCTGGCTGGCCCGTACACATTATAAGAGGACCATGAA AGCCATCGTCTACCTTCAGTGCTGCTTCCGGCGGATGATGGCCAAGCGTGAGCTGAAGAAACTCAAAATT GAGGCTCGCTCTGTGGAACGCTACAAGAAGCTCCATATTGGCATGGAGAACAAGATTATGCAGCTGCAGC GCAAAGTGGATGAGCAGAATAAAGACTACAAATGCCTCATGGAGAAACTGACCAATCTGGAAGGAGTATA CAACTCTGAGACTGAAAAACTACGAAATGATGTAGAACGTCTTCAGCTAAGTGAAGAGGAAGCTAAGGTT GCCACTGGGCGGGTCCTTAGTCTGCAGGAAGAAATTGCAAAACTCCGAAAAGACCTGGAACAAACTCGAT CAGAGAAAAAGTCTATTGAAGAAAGAGCAGATAAATACAAACAAGAAACTGACCAGCTGGTGTCAAACTT GAAGGAAGAAAATACTTTGCTGAAGCAGGAAAAGGAGACCCTCAACCACCGCATTGTGGAGCAGGCGAAG GAGATGACAGAAACTATGGAGAGGAAGTTAGTAGAAGAGACAAAACAACTGGAGCTTGACCTGAATGATG AGAGGCTGAGGTATCAGAACCTTCTGAATGAGTTCAGTCGCCTGGAGGAGCGCTATGATGACCTCAAGGA AGAGATGACCCTGATGCTGAATGTGCCTAAGCCAGGACACAAGAGAACAGACTCTACCCACAGCAGTAAT GAGTCTGAATACACCTTCAGCTCTGAGTTTGCAGAAACTGAAGACATTGCACCAAGAACAGAGGAGCCAA TTGAGAAGAAGGTGCCTCTGGATATGTCACTATTCCTTAAGCTCCAGAAGCGTGTCACAGAGCTGGAACA GGAGAAGCAGCTGATGCAGGATGAGCTGGACCGCAAGGAGGAGCAGGTGTTCCGCAGCAAGGCAAAGGAA GAAGAAAGGCCACAGATAAGAGGAGCTGAACTAGAGTATGAGTCTCTCAAGCGTCAAGAACTGGAGTCAG AAAACAAAAAACTGAAAAACGAGCTGAATGAGTTGCGCAAAGCCCTCAGTGAGAAAAGTGCCCCAGAAGT GACTGCGCCAGGTGCGCCTGCTTACCGAGTCCTCATGGAACAGCTGACCTCCGTGAGCGAGGAGCTCGAC GTGCGCAAGGAGGAAGTCCTCATCTTGAGGTCGCAGCTGGTGAGCCAAAAAGAAGCCATCCAACCCAAGG ATGACAAGAATACAATGACAGATTCCACAATTCTTTTAGAAGATGTACAGAAAATGAAAGACAAAGGTGA AATAGCACAAGCATATATTGGTTTGAAAGAAACAAACAGGCTCTTAGAATCCCAGCTACAGTCACAGAAA AGAAGCCATGAGAATGAGGCTGAGGCCCTCCGTGGGGAGATCCAGAGCCTAAAGGAAGAAAACAACCGGC AACAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCTGAGGCCCGCATTGAGGCCAGCCTGCAGCATGA GATCACCCGGCTGACCAATGAAAACCTGTATTTTGAGGAATTATATGCAGATGACCCTAAGAAGTATCAA TCCTATCGGATTTCACTTTACAAAAGGATGATTGATCTGATGGAACAACTTGAAAAGCAGGATAAAACTG TCCGGAAACTGAAGAAACAACTGAAAGTCTTTGCCAAAAAAATTGGTGAACTAGAAGTGGGGCAGATGGA GAACATATCTCCAGGACAGATCATCGATGAGCCTATCCGGCCAGTCAACATTCCCCGGAAAGAAAAGGAT TTCCAAGGAATGCTGGAGTACAAAAGGGAGGATGAGCAGAAGCTAGTGAAGAACCTGATTCTAGAACTAA AGCCACGTGGTGTGGCTGTCAATCTGATTCCAGGGTTACCAGCATATATCTTGTTTATGTGTGTACGACA TGCTGACTATCTGAACGATGATCAGAAAGTAAGATCATTGCTGACATCAACAATTAACAGCATCAAAAAA GTCTTGAAGAAAAGAGGTGACGATTTTGAAACTGTCTCCTTCTGGCTCTCTAACACATGTCGATTTTTGC ACTGTTTGAAGCAATATAGTGGAGAGGAGGGCTTTATGAAGCACAACACGTCTCGCCAGAATGAGCACTG CCTCACCAATTTTGACCTTGCTGAATATCGGCAAGTACTGAGTGACTTGGCCATTCAGATCTATCAGCAG CTTGTGAGGGTGTTAGAGAACATTCTTCAGCCAATGATAGTCTCAGGTATGCTAGAGCATGAAACAATTC AGGGCGTATCTGGGGTGAAGCCCACAGGGCTCAGAAAACGAACCTCCAGTATCGCAGATGAGGGCACCTA CACACTGGACTCCATCCTTCGGCAGCTCAACTCCTTCCACTCAGTCATGTGTCAGCATGGCATGGACCCG GAGCTAATCAAGCAGGTAGTCAAACAGATGTTCTACATTGTGGGCGCCATCACCCTAAACAACCTCCTCC TGCGCAAGGACATGTGCTCCTGGAGCAAGGGCATGCAGATCAGGTACAATGTCAGTCAACTGGAAGAATG GCTACGTGACAAGAATCTAATGAACAGTGGGGCCAAGGAGACTCTGGAACCTCTTATCCAGGCCGCTCAG CTTTTGCAAGTGAAAAAGAAAACTGACGATGACGCAGAAGCCATCTGTTCCATGTGCAACGCCCTGACCA CGGCCCAGATTGTCAAAGTGTTGAATCTGTACACACCGGTTAATGAGTTTGAAGAAAGGGTCTCAGTTTC ATTTATCCGCACTATACAGATGCGGTTACGAGACAGGAAAGACTCTCCACAGCTGCTCATGGATGCAAAA CACATCTTTCCTGTCACTTTTCCCTTTAACCCATCCTCCCTAGCCCTAGAAACCATCCAGATCCCAGCCA GCCTGGGCCTGGGCTTCATCGCACGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221640 representing NM_010864 Red=Cloning site Green=Tags(s) MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYRLDPKTGELPHLRNPDILVG ENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIF AVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAK TTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLG NADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILHLGNVGFASRDSDSCTI PPKHEPLTIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDHVN QALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFY DNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQ CEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRPGQTAKE HKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSR WTYQEFFSRYRVLMKQKDVLGDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAAC IRIQKTIRGWLLRKRYLCMQRAAITVQRYVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRRYKIRRAA TIVIQSYLRGYLTRNRYRKILREYKAVIIQKRVRGWLARTHYKRTMKAIVYLQCCFRRMMAKRELKKLKI EARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQLSEEEAKV ATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETDQLVSNLKEENTLLKQEKETLNHRIVEQAK EMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMLNVPKPGHKRTDSTHSSN ESEYTFSSEFAETEDIAPRTEEPIEKKVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVFRSKAKE EERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELD VRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQK RSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQ SYRISLYKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKD FQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKK VLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQ LVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDP ELIKQVVKQMFYIVGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQ LLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAK HIFPVTFPFNPSSLALETIQIPASLGLGFIARV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010864
ORF Size	5559 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010864.2, NP_034994.2
RefSeq Size	11684 bp
RefSeq ORF	5562 bp
Locus ID	17918
UniProt ID	Q99104
MW	215.5 kDa
Gene Summary	Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.[UniProtKB/Swiss-Prot Function]

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