Wnk1 (NM_001185020) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221512 representing NM_001185020 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGGCACAGGGGCAGAACCAGGGCCAGCCAT CAAGTAGTTTGGCTGGGGTTCTATCTTCACAACCTATCCAACATCCTCAGCAGCAGGGAATACAGCCAAC TGTTCCTTCTCAACAGGCAGTACAGTATTCACTTCCACAAGCAGCATCTTCCAGTGAAGGTACTACTGCA CAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTACTCAGGGAGTCTCTCAGGCTGCTCCTC CAGAACAAACTCCAATAACACAGTCACAACCTACTCAGCCTGTTCCATTGGTTACCTCTGCAGACAGTGC ACATTCAGACGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCTCCATCATCCAGCGGGAGGCATGAA GGGAGGACAACAAAACGGCATTATAGAAAATCCGTAAGAAGTCGCTCTCGGCATGAAAAGACTTCTCGCC CAAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAA TCGGAAGATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTG AACAATGACTTTATTCTAGCAATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGG CTGATGAAATGTTAAGTGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTGCAAGG AAAGGATGACTATGGCTTCCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCT TCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACGCAAGTTGTTCATTCTGCTGGAAGACGGT TTATAGTGAGTCCTGTACCTGAAAGTAGGTTACGAGAATCAAAAGTTTTCACCAGTGACATATCAGATCC AGTTGTGGCCTCTACATCTCAGGCCCCTGGAATGAACTTATCTCACTCTGCTTCATCCCTCAGTCTCCAG CAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATC ACATGGCGGGACCAACATTTTCTCCCTTTTTGGCTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCAC ACCGTCAGTATCAGTCCCTATAACAAGCAGTCCTCTTAATGACATTTCCACATCAGTGATGCAGTCTGAG ACTGCACTGCCCACTGAAAAAGGCATCGTGGGAGTTACCACTACCAGCACAGGTGTGGTAGCTTCAGGTG GTCTCACCACAATGTCTGTGTCTGAGTCACCAACATCTTCCAGTGCGGTTTCAAGTTCTACAGTACCTGC AGTTGTCACTGTGTCCACACCATCCCAGCCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCAAGTACA GGCAGTTTTCCTCCTGGAACATTTTCTACAACTACAGCTACTACAATGGGTAGTGTGGTGGCCCCAGATG CTAAGCCTCCAACTGTATTATTGCAACAGGTAGCAAGCAATACTGCTGGGGTTGCCATAGTGACATCAGT CTCTACCACCACTCCATTCCCAGGCATGGCTTCACAGCCATCCCTTCCACTTAGTAGCAGCACCTCTGCT CCTACTCTAGCTGAAACAATGGTGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAG GATTGGGTTTGTCCTTTTGTGCGCCATCCTCTTCCTCCTCCTCTGGAACAGCTGTGTCTACTTCTGTTTC TCAGCCTGGTATGGTTCATCCTTTGGTCATCTCATCAGCTGTAGTTTCTACACCTGGCCTCCCCCAACCA GTAGTACCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGTTA ATGTGCCTGCTGTACAGCAAACACTCATTCACAGTCAGCCTCAACCAGCTCTGCTTCCCAACCAGCCTCA CACACACTGTCCTGAAATGGATGCTGATACCCAATCCAAAGCTCCTGGGATTGATGATATAAAGACTCTG GAGGAAAAGCTGAGATCTCTCTTTAGTGAACACAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTAG AGACACCTTTAGTAGTAGAGACGACTGTCACACCGGGCATCACTACCACTGCTGTAGCACCAAGTAAACT CATGACTTCCACTACAAGTACTTGCTTACCACCAACCAGTTTACCATTAGGAGCAGCTGGCATGCCAGTT ATGCCAGTAGGCACACCTGGGCAGGTTTCTACCCCAGGCACACACGCTTCAGCCCCAGTCGGCACTGCAA CAGGAGTAAAACCTGGAACAACTCCCCCAAAGCCAACTAAGACTGTGGTGCCACCAGTGGGTACTGAACT TTCAGCCGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCTGGACCTTCTCTAATTCAGTCCCAGCAA CCACTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTACAGTGGCACCTGCAG TTGGTCCTTTGTCTACGATGTCTTCAACAACAGTTACAGAAGCAGGGACTCGGCTTCAAAAGGATGGCAC AGAAGGTCATGTCACAGCAACTAGTTCAGGAGCTGGTGTTGTTAAGATGGGGCGATTTCAGGTTTCAGTT ACAATGGATGATGCCCAGAAAGAACGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTA GCACATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGG AATTAGTATTTCTGGCATCTCCTTAGATGTCCCAGACAGCACCCACAAAGCACCTACCCCAGAAGCAAAG TCAGATGCAGGACAGCCTACCAAGGTGGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTT TCTCTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTCCTTT CAGAGATTCAGAACAAACTGTTATTCCTGCTGTGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCT TCCCATTTGAATGGGCCATCTTCAGACCTGGAGGCTGCCTTTCTAAGTAGGGGTACAGAGGATGGCTCAG GTAGTCCACACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCGTCCAGAATCTAAGTCAAAGCCTTAG TAATTCATTCAACTCCTCATACATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTA GAACTACGACGACTACGAGAAAAACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAA TTGAATCTTTGTATACTAAACTGGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTC GGGGAGAAGAAGGAGACCCACTAAAAGCAAAGGCAGCAAGTCTAGTCGCAGCAGCTCATTGGGCAATAAA AGCCCACAGCTTTCAGGCAACCTGTCTGGTCAGAGTGGAACTTCAGTCTTACACCCCCAACAGACCCTCC ATCCCGCAGGAAACACCCCCGAGACTGGGCACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAG TGACAACCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAA GGGACCAGCAGCACAAATACCGTTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCTA TGACTTCCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTTAGTAGACAACTGGGCCCGAGATGC CATGAATCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAG TTCTCTGCTCCTGGGCAGCTGTGTGTTCCCATGACCTCAAACCTGGGTGGCTCTACCCCCATCTCTGCAG CATCTGCTACCTCTCTAGGTCACTTCACCAAATCCATGTGTCCCCCACAGCAGTATGGTTTTCCACCTGC CCCATTTGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCGCAGCCACTTGGCCAGTTCCAACCTGTA GGAACAGCATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCA ATCTACGGACCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221512 representing NM_001185020 Red=Cloning site Green=Tags(s) MSDGAAEKQSGTPGFLTPPAPVPKNGSSSDSSVGEKLGATVADSGVGRTEEYRRRRHTMDKDSRGAAATT TPTEHRFFRRSVICDSNATALELPGLPLSIPQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAAS PGEQAVVGSATTTVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEAQEDRSQQQDDIEELETK AVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNI VRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELA EEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKD RVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQQLSAASTGIPTAPATSASVSTQVEP EEPEADQHQQLQYQQPSISVLSDGTIDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTAPGHTVSSIQ AQSQPHGVYPPSSMAQGQNQGQPSSSLAGVLSSQPIQHPQQQGIQPTVPSQQAVQYSLPQAASSSEGTTA QPVSQPQVSAGTQSTQGVSQAAPPEQTPITQSQPTQPVPLVTSADSAHSDVASGMSDGNENAPSSSGRHE GRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMV NNDFILAIERESFVAQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFPGSQKLEGEFKQPIAVS SMPQQIGVPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSDISDPVVASTSQAPGMNLSHSASSLSLQ QAFSELKHGQMTEGPNTAPPNFNHMAGPTFSPFLASIAGVQTVAASTPSVSVPITSSPLNDISTSVMQSE TALPTEKGIVGVTTTSTGVVASGGLTTMSVSESPTSSSAVSSSTVPAVVTVSTPSQPVQASTSGSIASST GSFPPGTFSTTTATTMGSVVAPDAKPPTVLLQQVASNTAGVAIVTSVSTTTPFPGMASQPSLPLSSSTSA PTLAETMVVSAHSLDKASHSSTAGLGLSFCAPSSSSSSGTAVSTSVSQPGMVHPLVISSAVVSTPGLPQP VVPTSTPLLPQVPNIPPLVQPVVNVPAVQQTLIHSQPQPALLPNQPHTHCPEMDADTQSKAPGIDDIKTL EEKLRSLFSEHSSSGTQHASVSLETPLVVETTVTPGITTTAVAPSKLMTSTTSTCLPPTSLPLGAAGMPV MPVGTPGQVSTPGTHASAPVGTATGVKPGTTPPKPTKTVVPPVGTELSAGTVPCEQLPPFPGPSLIQSQQ PLEDLDAQLRRTLSPETITVAPAVGPLSTMSSTTVTEAGTRLQKDGTEGHVTATSSGAGVVKMGRFQVSV TMDDAQKERKNRSEDTKSVHFESSTSESSVLSSSSPESTLVKPEPNGISISGISLDVPDSTHKAPTPEAK SDAGQPTKVGRFQVTTTANKVGRFSVSRTEDKVTELKKEGPVTSPPFRDSEQTVIPAVIPKKEKPELAEP SHLNGPSSDLEAAFLSRGTEDGSGSPHSPPHLCSKSLPVQNLSQSLSNSFNSSYMSSDNESDIEDEDLRL ELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNK SPQLSGNLSGQSGTSVLHPQQTLHPAGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQ GTSSTNTVGGTVSSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARK FSAPGQLCVPMTSNLGGSTPISAASATSLGHFTKSMCPPQQYGFPPAPFGTQWSGTGGPAPQPLGQFQPV GTASLQNFNISNLQKSISNPPGSNLRTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001185020
ORF Size	6384 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001185020.1, NP_001171949.1
RefSeq Size	9828 bp
RefSeq ORF	6387 bp
Locus ID	232341
UniProt ID	P83741
MW	225.9 kDa
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function]

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