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Celf1 (NM_017368) Mouse Tagged ORF Clone
CAT＃: MR220707
- TrueORF®
Celf1 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1
ORF Plasmid: tGFP
"NM_017368" in other vectors (4)
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CNY 3,824.00
CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1600010O03Rik; AA407467; Brunol2; CUG-BP; CUG-BP1; CUGBP; Cugbp1; D2Wsu101e; HNAB50; NAB50 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220707 representing NM_017368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGTTTAAGTTGGATTTCCTTCCAGAAATGATGGTGGATCATTGCTCTTTGAATTCCAGTCCTG TCTCAAAGAAAATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGT GGGCCAGGTTCCAAGGACCTGGTCTGAGAAAGACTTGAGGGAGCTTTTTGAGCAGTATGGTGCTGTCTAT GAAATCAACATCCTAAGGGATAGGAGCCAAAACCCTCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTT ACACCCGTAAAGCTGCATTAGAAGCTCAGAATGCTCTCCATAACATGAAGGTCCTCCCTGGGATGCATCA CCCTATACAGATGAAGCCTGCTGACAGTGAAAAGAACAATGCTGTGGAAGACAGGAAGCTCTTTATTGGT ATGATTTCCAAGAAGTGTACTGAAAATGACATCCGAGTCATGTTTTCTTCGTTTGGACAGATTGAAGAGT GCCGGATATTGCGGGGACCTGATGGCTTGAGCAGAGGTTGTGCATTTGTGACTTTTACTACAAGAACCAT GGCACAGACAGCTATCAAAGCAATGCACCAAGCACAAACCATGGAGGGTTGCTCATCCCCAATGGTGGTA AAGTTTGCCGACACCCAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAGCTCCAGCAGCAGATGCAGC AGATCAGTGCAGCGTCTGTGTGGGGAAACCTGGCTGGTCTGAACACACTTGGACCCCAGTACTTAGCACT TTATTTGCAGCTCCTACAGCAGACTGCCTCCTCTGGGAACCTCAACACCCTGAGCAGCCTCCACCCAATG GGAGGGTTAAATGCAATGCAGCTACAGAATTTGGCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACA CACCAAGTGGTACCAATGCTCTCACTACATCCAGCAGTCCCCTCAGCGTACTCACCAGTTCAGGGTCTTC ACCGAGCTCCAGCAGCAGTAATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAAACATTGGCCGGA GCAACAGCTGGCCTCAACGTTGGCTCATTGGCAGGGATGGCTGCTTTGAATGGTGGTCTGGGCAGCAGTG GCCTTTCCAATGGCACTGGGAGTACCATGGAAGCCCTCACCCAGGCGTATTCTGGTATCCAGCAATATGC TGCTGCAGCCCTCCCCACTCTGTACAACCAGAATTTATTGACACAGCAGAGTATTGGTGCTGCTGGAAGC CAGAAGGAAGGTCCAGAAGGAGCCAACCTGTTCATCTATCACCTGCCCCAGGAGTTTGGAGACCAGGACT TACTGCAGATGTTTATGCCCTTTGGGAATGTCGTGTCGGCCAAGGTTTTCATAGACAAGCAGACAAACCT GAGCAAGTGCTTTGGTTTTGTAAGTTATGACAATCCTGTCTCAGCTCAAGCTGCCATCCAGTCCATGAAC GGCTTTCAAATTGGAATGAAGAGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGATAGTAAGCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220707 representing NM_017368
Red=Cloning site Green=Tags(s) MAAFKLDFLPEMMVDHCSLNSSPVSKKMNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVY EINILRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNAVEDRKLFIG MISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRTMAQTAIKAMHQAQTMEGCSSPMVV KFADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTASSGNLNTLSSLHPM GGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSGSSPSSSSSNSVNPIASLGALQTLAG ATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGS QKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMN GFQIGMKRLKVQLKRSKNDSKPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017368 |
| ORF Size | 1539 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017368.3, NP_059064.2 |
| RefSeq Size | 4873 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 13046 |
| UniProt ID | P28659 |
| MW | 55.1 kDa |
| Gene Summary | RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing (By similarity). Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition (By similarity). Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs (By similarity). Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB (By similarity). Promotes exclusion of exon 11 of the INSR pre-mRNA (By similarity). Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast (By similarity). Increases translation and controls the choice of translation initiation codon of CEBPB mRNA (By similarity). Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3 (By similarity). Mediates rapid cytoplasmic mRNA deadenylation (By similarity). Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation (By similarity). Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3' UTR of transcripts such as DMPK and to Bruno response elements (BREs) (By similarity). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity). Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3' UTR of JUN and FOS mRNAs. Binds to the IR RNA (By similarity). Binds to the 5'-region of CDKN1A and CEBPB mRNAs (By similarity). Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217278 | Celf1 (untagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1, (10ug) |
CNY 3,832.00 |
|
| MG220707 | Celf1 (tGFP-tagged) - Mouse CUGBP Elav-like family member 1 (Celf1) transcript variant 1, (10ug) |
CNY 4,940.00 |
|
| MR220707L3 | Lenti ORF clone of Celf1 (Myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1 |
CNY 6,370.00 |
|
| MR220707L4 | Lenti ORF clone of Celf1 (mGFP-tagged) - Mouse CUGBP, Elav-like family member 1 (Celf1), transcript variant 1 |
CNY 6,370.00 |
