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Elmo1 (NM_080288) Mouse Tagged ORF Clone
CAT＃: MR220091
- TrueORF®
Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1
ORF Plasmid: tGFP
"NM_080288" in other vectors (4)
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CNY 5,320.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330578D22Rik; C230095H21Rik; CED-12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220091 representing NM_080288
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCGCCTTCGGACATCGTGAAGGTGGCCATAGAATGGCCTGGCGCCTACCCTAAACTCATGGAGA TTGACCAGAAAAAGCCACTGTCTGCCATTATAAAGGAAGTCTGTGATGGGTGGTCTCTTGCCAACCATGA ATATTTTGCACTGCAGCACGCTGATAGTTCAAACTTCTACATCACAGAAAAGAATCGCAACGAAATAAAA AATGGCACGATCCTTCGATTAACCACATCTCCAGCTCAGAATGCCCAGCAGCTCCATGAACGAATACAGT CATCAAGTATGGATGCCAAGTTGGAAGCCCTGAAGGACTTGGCCAGCCTCTCCCGGGATGTTACCTTTGC TCAGGAGTTCATAAACCTGGATGGCATCTCACTTCTCACTCAGATGGTGGAAAGTGGCACTGAACGATAC CAGAAGTTGCAGAAGATTATGAAGCCCTGCTTTGGAGACATGCTGTCCTTCACTCTCACAGCCTTTGTGG AGTTGATGGACCACGGCATTGTATCATGGGATACATTCTCAGTGGCCTTCATTAAGAAGATAGCCAGCTT TGTGAACAAGTCTGCCATAGACATCTCAATCCTCCAGCGGTCCTTGGCCATTCTGGAGTCAATGGTTCTC AACAGTCATGACCTCTACCAAAAGGTAGCCCAGGAGATCACCATTGGCCAACTCATTCCTCATCTTCAGG GGACAGACCAAGAAATACAAACTTACACCATTGCTGTAATCAACGCACTTTTCCTCAAAGCTCCTGATGA GAGGAGACAGGAGATGGCGAATATTTTGGCTCAGAAGCAGCTGCGCTACATCATTTTAACACATGTCATC CGAGCACAGCGGGCCATCAACAATGAGATGGCACACCAGTTATATGTTCTCCAAGTGCTGACCTTTAACC TTCTGGAAGACAGGATGATGACTAAGATGGACCCCCAGGACCAGGCCCAAAGGGACATCATATTTGAACT TCGAAGAATTGCTTTCGATGCAGAGTCAGAACCTAATAACAGCAGTGGCAGCATGGAGAAACGCAAGTCC ATGTACACTCGGGATTATAAAAAACTTGGCTTCATTAATCACGTCAATCCTGCCATGGACTTCACACAGA CCCCTCCTGGGATGCTGGCTCTGGACAACATGCTGTATTTCGCTAAGCACCACCAGGATGCATACATCCG GATCGTGCTAGAGAACAGCAGCCGAGAAGATAAACATGAGTGCCCCTTCGGCCGCAGCAGTATAGAGCTG ACCAAGATGCTGTGTGAGATCCTCAAAGTGGGCGAGCTGCCTAGTGAGACCTGCAACGACTTTCACCCGA TGTTCTTCACCCATGACAGATCTTTTGAGGAATTCTTCTGCATTTGCATTCAACTCCTGAACAAAACATG GAAGGAAATGAGGGCAACATCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGA GCGCTTACAACCAAGCCTAGTTCCCTGGACCAGTTCAAAAGCAAGCTGCAAAACCTGAGCTACACCGAGA TCCTGAAAATCCGCCAGTCTGAGAGGATGAACCAGGAAGATTTCCAGTCTCGCCCGATTTTGGAACTAAA GGAGAAGATCCAGCCAGAAATCTTAGAGCTGATTAAACAGCAGCGCCTGAACCGCCTTGTGGAAGGGACC TGCTTTAGGAAACTCAATGCTCGCCGGAGACAAGACAAGTTTTGGTATTGTCGGCTTTCACCAAATCACA AGGTCTTACATTATGGCGACTTGGAAGAGAGCCCCCAAGGAGAAGTGCCCCACGATTCCCTGCAGGACAA ATTGCCGGTGGCAGATATCAAAGCAGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCC CTTAAACAAAACAAGGAGGTGCTTGAACTTGCTTTCTCCATCTTATACGACTCAAATTGCCAACTGAACT TCATTGCTCCTGATAAGCATGAGTACTGCATCTGGACAGATGGGCTGAATGCACTGCTTGGGAAGGACAT GATGAGTGACCTGACACGCAATGACCTGGACACCCTGCTGAGCATGGAGATCAAGCTTCGCCTGCTGGAC CTGGAAAACATCCAGATCCCGGATGCACCTCCGCCTATCCCCAAGGAACCTAGCAACTATGACTTTGTCT ATGACTGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220091 representing NM_080288
Red=Cloning site Green=Tags(s) MPPPSDIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEIK NGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERY QKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILESMVL NSHDLYQKVAQEITIGQLIPHLQGTDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRYIILTHVI RAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKS MYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIEL TKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMR ALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGT CFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGA LKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLD LENIQIPDAPPPIPKEPSNYDFVYDCN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080288 |
| ORF Size | 2181 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080288.2, NP_525027.1 |
| RefSeq Size | 5576 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 140580 |
| UniProt ID | Q8BPU7 |
| MW | 83.9 kDa |
| Gene Summary | Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221117 | Elmo1 (untagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, (10ug) |
CNY 9,310.00 |
|
| MG220091 | Elmo1 (tGFP-tagged) - Mouse engulfment and cell motility 1 ced-12 homolog (C. elegans) (Elmo1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR220091L3 | Lenti ORF clone of Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1 |
CNY 7,700.00 |
|
| MR220091L4 | Lenti ORF clone of Elmo1 (mGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1 |
CNY 7,700.00 |
