Ubr2 (NM_001177374) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9930021A08Rik; AI462103; AW540746; E130209G04Rik; ENSMUSG00000043296; mKIAA0349
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219537 representing NM_001177374 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGAGATGGAGCCCGAGGTGCAGGCCATCGACCGCAGTTTGCTGGAATGTTCTGCCGAAGAGA TCGCAGGGAGATGGCTGCAAGCAACCGACCTCAACAGAGAAGTGTACCAGCATTTAGCCCACTGTGTGCC CAAAATCTACTGCCGGGGCCCTAACCCCTTCCCTCAGAAGGAAGACACGCTGGCACAGCACATCCTGCTG GGACCGATGGAGTGGTACATCTGCGCTGAAGACCCTGCGCTGGGATTTCCAAAGCTCGAGCAGGCAAACA AGCCTTCTCACCTCTGTGGCCGAGTGTTTAAAGTGGGGGAACCTACATACTCCTGCAGAGACTGTGCAGT TGACCCCACCTGTGTTTTATGCATGGAGTGCTTCCTGGGAAGTATCCATAGAGACCATCGATATAGGATG ACCACATCGGGAGGAGGGGGCTTCTGTGACTGTGGTGACACTGAGGCGTGGAAAGAGGGACCTTACTGCC AGAAGCACAAGCTCAGCAGCTCTGAAGTTGTGGAGGAGGAGGATCCTCTTGTGCATCTATCAGAAGATGT GATCGCCAGAACTTACAACATTTTTGCTATTATGTTTCGATATGCAGTAGATATACTGACCTGGGAAAAA GAAAGTGAATTGCCTGAAGACTTAGAAGTGGCAGAGAAGAGTGACACCTACTACTGCATGCTGTTTAATG ATGAGGTTCACACCTATGAGCAAGTCATTTATACCCTTCAGAAAGCTGTGAACTGTACACAGAAGGAAGC CATTGGCTTTGCAACTACAGTTGATCGAGATGGCCGTAGGTCTGTCCGATATGGAGATTTCCAGTACTGT GATCAAGCAAAGACAGTCATTGTGAGGAACACCAGCAGACAGACCAAGCCGCTCAAAGTTCAAGTTATGC ACTCCTCCGTGGCTGCTCATCAGAATTTTGGTTTGAAAGCTCTGTCGTGGCTGGGAAGTGTTATTGGATA CTCAGATGGCCTTCGCAGGATTTTGTGTCAAGTTGGATTACAAGAAGGTCCAGATGGCGAAAACTCTTCT CTGGTCGACAGACTGATGCTTAATGATTCCAAATTATGGAAAGGGGCTAGGAGTGTGTATCACCAGTTGT TCATGAGCAGCCTGCTCATGGACCTCAAGTATAAGAAGCTGTTCGCGCTTCGATTTGCTAAAAACTATGA GCGTTTGCAGAGAGATTATGTGACAGATGACCACGACAGAGAGTTTTCAGTCGCAGACCTCTCGGTTCAG ATATTCACAGTTCCTTCACTTGCGCGAATGCTCCTCACAGAAGAGAACCTGATGACCGTTATCATTAAGG CTTTCATGGACCATTTGAAACACAGAGATGCCCAGGGCAGATTCCAGTTTGAACGCTACACTGCCCTCCA AGCCTTCAAGTTCAGGAGAGTCCAGAGCCTCATCTTAGATCTCAAGTATGTATTGATTAGCAAACCAACG GAGTGGTCAGATGAGCTGAGGCAGAAGTTCTTACAAGGGTTCGATGCCTTCTTGGAATTACTGAAGTGCA TGCAGGGAATGGACCCGATCACGCGTCAGGTGGGACAGCACATTGAGATGGAGCCAGAGTGGGAAGCAGC CTTCACACTGCAGATGAAGCTGACACACGTCATCTCAATGGTGCAGGACTGGTGTGCTCTGGACGAAAAA GTGTTAATTGAAGCTTACAAGAAATGCCTGGCTGTGCTGACACAGTGTCATGGCGGATTTACTGATGGTG AACAGCCAATCACACTCAGTATTTGTGGACACTCGGTGGAAACCATCAGATACTGTGTTTCCCAAGAAAA AGTTAGCATTCACCTCCCAATTTCTCGCTTGCTTGCAGGTTTGCATGTATTGTTAAGCAAAAGTGAAGTG GCATATAAATTTCCAGAGCTCCTACCTCTAAGTGAACTGAGCCCACCCATGTTGATAGAACATCCTCTTA GATGTCTTGTCTTATGTGCTCAAGTGCATGCTGGGATGTGGAGAAGAAATGGCTTCTCTCTAGTAAATCA GATCTATTACTACCATAATGTGAAATGCAGGCGAGAGATGTTCGACAAGGACATAGTGATGCTTCAGACA GGTGTCTCCATGATGGACCCAAACCACTTCCTGATGATCATGCTCAGCCGCTTTGAACTCTATCAGCTCT TCAGCACGCCTGACTATGGGAAGAGATTCAGTTCTGAGGTTACCCATAAGGACGTCGTTCAGCAGAACAA CACTCTGATCGAAGAGATGCTCTACCTCATCATCATGCTTGTGGGAGAAAGATTCAACCCTGGGGTTGGA CAGGTGGCTGCCACAGATGAAATCAAGAGGGAGATTATCCATCAGTTGAGCATCAAGCCTATGGCTCACA GTGAGCTGGTGAAGTCTCTGCCTGAAGATGAGAACAAGGAGACCGGCATGGAGAGCGTCATCGAGTCCGT TGCACATTTCAAGAAACCTGGGCTCACAGGGCGAGGCATGTATGAGCTGAAGCCAGAGTGTGCCAAAGAG TTCAACCTGTATTTTTATCATTTCTCCAGGGCAGAGCAGTCCAAGGCAGAGGAAGCTCAGCGGAAATTGA AAAGAGAAAATAAAGAAGATACAGCACTCCCTCCTCCGGCTTTGCCACCGTTCTGCCCTTTGTTCGCGAG TCTGGTTAACATCTTGCAGTGTGACGTCATGCTGTACATCATGGGAACGATCCTGCAGTGGGCTGTAGAG CATCACGGGTCTGCCTGGTCAGAGTCCATGCTACAGAGGGTGCTGCATTTGATCGGGATGGCTCTCCAGG AAGAGAAGCACCACTTGGAGAACGCCGTGGAAGGGCACGTGCAGACCTTCACCTTCACACAGAAGATTTC AAAGCCTGGTGATGCACCACATAACTCCCCGAGCATCCTAGCTATGCTGGAGACCTTGCAGAACGCCCCC TCCCTGGAAGCCCACAAGGACATGATCAGGTGGTTGCTAAAGATGTTTAATGCAATTAAGAAGATAAGAG AGTGTTCATCCAGCAGCCCTGTGGCCGAGGCGGAGGGAACCATAATGGAGGAGAGCTCAAGAGACAAGGA CAAAGCAGAGAGGAAAAGAAAAGCCGAGATCGCCAGACTGCGCCGGGAGAAGATCATGGCCCAGATGTCT GAGATGCAGCGGCACTTCATTGACGAAAACAAAGAGCTCTTCCAGCAGACCCTAGAGCTGGACACCTCTG CCTCTGCCACTCTTGACAGCAGCCCTCCCGTTTCAGACGCAGCTCTTACAGCACTGGGCCCAGCACAGAC ACAGGTCCCTGAACCGAGACAGTTTGTCACCTGTATATTATGTCAAGAGGAGCAAGAGGTGACTGTGGGA AGCAGGGCGATGGTCTTGGCAGCGTTTGTTCAGAGGTCAACGGTTCTGTCAAAAGACAGGACGAAAACCA TCGCGGACCCAGAAAAATATGATCCATTATTCATGCACCCCGATCTGTCTTGTGGGACACACACTGGCAG CTGTGGGCACGTTATGCATGCCCATTGTTGGCAAAGGTATTTTGATTCCGTTCAAGCCAAGGAGCAGCGA AGGCAGCAGCGGCTGCGCTTGCACACTAGCTACGATGTAGAGAATGGCGAGTTCCTCTGCCCGCTCTGTG AGTGCCTGAGCAACACGGTGATCCCCCTGCTGCTTCCTCCCAGGAGCATCCTCAGCAGGAGGTTAAATTT TTCAGACCAACCAGATCTGGCACAGTGGACGAGAGCAGTAACACAGCAGATAAAGGTGGTCCAGATGCTG AGGAGAAAGCACAATGCTGCTGACACGTCTTCTTCAGAGGACACAGAAGCCATGAATATAATACCGATCC CCGAAGGCTTCAGGCCTGATTTTTATCCTAGGAACCCATATTCTGATAGCATAAAAGAAATGTTAACGAC ATTTGGAACGGCTGCTTACAAGGTGGGACTGAAGGTTCATCCTAATGAAGGTGACCCCCGTGTGCCCATC CTGTGCTGGGGGACCTGTGCATACACCATCCAGAGCATAGAAAGAATTTTGAGTGATGAGGAGAAGCCTG TTTTTGGACCTCTGCCTTGTAGACTGGACGACTGTCTCAGGTCGTTAACACGGTTTGCAGCAGCACATTG GACAGTGGCGTTACTTCCTGTGGTACAAGGACACTTCTGTAAACTCTTTGCATCCTTGGTGCCTAGTGAC AGCTATGAAGACCTCCCGTGCATACTAGACATCGACATGTTTCACTTGCTGGTGGGCCTGGTGCTCGCTT TCCCAGCTCTGCAGTGTCAGGATTTTTCAGGAAGCAGCCTGGCCACTGGGGACCTGCACATCTTCCACTT GGTTACCATGGCACACATCGTACAGATCTTACTTACCTCATGTACAGAAGAGAATGGCATGGATCAAGAG AATCCCACTGGGGAAGAAGAACTGGCCATTCTCTCTTTGCACAAAACACTTCACCAGTATACTGGAAGTG CCTTGAAAGAAGCCCCCTCCGGCTGGCACCTGTGGAGGAGCGTCCGGGCCGCCATCATGCCTTTCCTCAA GTGCTCTGCTTTGTTTTTCCACTATTTAAATGGAGTCCCGGCCCCTCCAGACCTTCAAGTTTCTGGAACA AGCCATTTTGAACACTTATGTAACTACCTTTCCCTACCAACCAACCTCATTCACCTTTTTCAAGAAAACA GTGACATCATGAACTCCCTGATTGAAAGTTGGTGCCAGAACAGTGAAGTTAAACGGTATCTAAATGGCGA GAGAGGAGCGATAAGCTACCCCAGAGGAGCTAACAAACTGATAGACCTTCCAGAGGATTACAGCAGCCTC ATTAACCAAGCATCCAATTTCTCGTGCCCCAAATCAGGTGGCGACAAGAGCAGAGCTCCTACTCTGTGCC TCGTGTGTGGGAGTCTCCTCTGCTCTCAGAGTTACTGCTGCCAAGCTGAGCTGGAGGGTGAGGACGTCGG AGCCTGCACAGCACACACCTACTCCTGCGGCTCCGGGGCCGGCATCTTCCTGAGAGTGCGGGAATGTCAG GTGCTATTTTTAGCTGGCAAAACCAAAGGATGTTTTTATTCTCCTCCTTACCTTGACGACTATGGAGAGA CCGACCAGGGACTCAGACGAGGAAATCCTTTACATTTATGCCAAGAGCGGTTTCGAAAGATCCAGAAGCT CTGGCAGCAGCATAGTATCACAGAGGAGATCGGACACGCGCAGGAGGCTAACCAGACCCTGGTCGGAATT GACTGGCAGCATTTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219537 representing NM_001177374 Red=Cloning site Green=Tags(s) MASEMEPEVQAIDRSLLECSAEEIAGRWLQATDLNREVYQHLAHCVPKIYCRGPNPFPQKEDTLAQHILL GPMEWYICAEDPALGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRM TTSGGGGFCDCGDTEAWKEGPYCQKHKLSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDILTWEK ESELPEDLEVAEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYC DQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQVGLQEGPDGENSS LVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFALRFAKNYERLQRDYVTDDHDREFSVADLSVQ IFTVPSLARMLLTEENLMTVIIKAFMDHLKHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPT EWSDELRQKFLQGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMVQDWCALDEK VLIEAYKKCLAVLTQCHGGFTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPISRLLAGLHVLLSKSEV AYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQT GVSMMDPNHFLMIMLSRFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVG QVAATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKE FNLYFYHFSRAEQSKAEEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVE HHGSAWSESMLQRVLHLIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAP SLEAHKDMIRWLLKMFNAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMS EMQRHFIDENKELFQQTLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVG SRAMVLAAFVQRSTVLSKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQR RQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQML RRKHNAADTSSSEDTEAMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPI LCWGTCAYTIQSIERILSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSD SYEDLPCILDIDMFHLLVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQE NPTGEEELAILSLHKTLHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGT SHFEHLCNYLSLPTNLIHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSL INQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQ VLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGI DWQHL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001177374
ORF Size	5265 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001177374.1, NP_001170845.1
RefSeq Size	7696 bp
RefSeq ORF	5268 bp
Locus ID	224826
UniProt ID	Q6WKZ8
MW	199.6 kDa
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity). Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (PubMed:28708824). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retranstoposon mobilization (PubMed:28806172).[UniProtKB/Swiss-Prot Function]

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