Shank3 (NM_021423) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI841104; Shank3b; SHANK3c-3; SHANK3c-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218960 representing NM_021423 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCCCCGGGGCCAGCGCCGTGGTCGTGCGCGTCGGCATCCCGGACCTGCAACAAACGAAGTGCC TGCGTCTGGACCCAACCGCGCCCGTGTGGGCCGCCAAGCAGCGTGTGCTCTGCGCCCTCAATCATAGCCT TCAAGACGCGCTCAACTACGGGCTATTCCAGCCTCCCTCCCGGGGTCGCGCCGGCAAGTTCCTGGATGAA GAGCGGCTCTTACAGGACTACCCGCCTAACCTGGACACGCCCCTGCCCTATCTGGAGTTCCGATACAAGC GGAGAGTTTATGCCCAGAACCTCATAGATGACAAGCAGTTTGCAAAGCTACACACAAAGGCAAACCTGAA GAAGTTCATGGACTATGTCCAGCTACACAGCACAGATAAGGTGGCCCGCCTGCTGGACAAGGGGCTGGAC CCCAATTTCCATGACCCTGACTCAGGAGAGTGCCCTCTGAGCCTTGCGGCACAGTTGGACAACGCCACTG ACCTCCTGAAGGTTCTCCGCAACGGCGGTGCTCATCTGGACTTCCGGACCCGAGATGGGCTGACAGCCGT CCACTGTGCTACCCGCCAGCGGAACGCAGGGGCATTGACGACCCTGCTGGACCTGGGGGCTTCGCCTGAC TACAAGGACAGCCGCGGCCTGACGCCCCTGTACCATAGTGCCCTAGGGGGCGGGGATGCCCTCTGTTGCG AGCTGCTTCTCCATGATCATGCACAGCTGGGGACCACTGATGAGAATGGTTGGCAAGAGATCCATCAGGC CTGTCGCTTTGGACACGTGCAGCACCTGGAGCACCTTTTGTTCTATGGGGCCAACATGGGTGCTCAGAAT GCCTCGGGAAACACAGCCCTGCACATCTGTGCCCTCTACAACCAGGAGAGTTGCGCGCGCGTCCTGCTTT TCCGTGGTGCCAACAAGGACGTCCGCAATTACAACAGCCAGACAGCCTTCCAGGTGGCCATTATTGCAGG GAACTTTGAGCTTGCCGAGGTAATCAAGACCCACAAAGACTCCGATGTCGTACCATTCAGGGAAACCCCC AGCTATGCAAAGCGACGGCGTCTGGCTGGCCCGAGTGGCCTGGCATCCCCACGGCCCTTACAGCGCTCAG CCAGTGATATCAACCTGAAAGGTGATCAGCCCGCAGCTTCTCCAGGGCCCACTCTCCGAAGCCTCCCTCA TCAACTCTTGCTCCAGAGGCTTCAGGAGGAGAAAGACCGTGACAGGGATGGTGAACTGGAGAATGACATC AGCGGCCCCTCAGCAGGCAGGGGTGGCCACAACAAGATCAGCCCCAGTGGGCCCGGCGGATCCGGCCCCG CGCCCGGCCCCGGCCCGGCGTCTCCCGCGCCCCCCGCGCCGCCGCCCCGGGGCCCGAAGCGGAAACTTTA CAGTGCCGTCCCCGGCCGCAAGTTCATCGCTGTGAAGGCGCACAGCCCGCAGGGCGAGGGCGAGATCCCG CTGCACCGCGGCGAGGCCGTGAAGGTGCTCAGCATTGGGGAGGGCGGTTTCTGGGAGGGAACCGTGAAGG GCCGAACAGGCTGGTTCCCAGCTGACTGTGTGGAAGAAGTGCAGATGCGACAGTATGACACCCGGCATGA AACCAGAGAGGACCGGACGAAGCGTCTCTTCCGCCACTACACTGTGGGTTCCTATGACAGCCTCACTTCA CACAGCGATTATGTCATCGATGATAAGGTGGCTATCCTGCAGAAAAGGGACCATGAGGGGTTTGGCTTTG TTCTCCGGGGAGCCAAAGCAGAGACCCCCATTGAGGAGTTTACACCCACACCTGCCTTCCCTGCACTCCA ATACCTTGAGTCTGTAGATGTGGAAGGTGTGGCCTGGAGGGCTGGACTTCGAACTGGGGACTTCCTCATT GAGGTGAACGGAGTGAATGTCGTGAAGGTTGGACACAAGCAAGTGGTGGGTCTCATCCGTCAGGGTGGCA ACCGCCTGGTCATGAAGGTTGTGTCTGTGACCAGGAAACCCGAGGAGGATGGTGCTCGGCGCAGAGCCCC ACCACCCCCAAAGAGGGCTCCCAGCACCACGCTGACCCTGCGGTCCAAGTCCATGACGGCTGAGCTCGAG GAACTTGCTTCCATTCGGAGAAGAAAAGGGGAGAAGTTGGATGAGATCCTGGCAGTTGCCGCGGAGCCGA CACTGAGGCCGGACATTGCAGATGCTGACTCGAGGGCGGCCACTGTCAAGCAGCGGCCCACCAGCCGGAG GATCACCCCTGCTGAGATCAGCTCATTGTTTGAGCGCCAGGGCCTCCCAGGCCCAGAGAAGCTGCCGGGC TCTCTGCGGAAGGGGATTCCACGGACCAAATCTGTAGGGGAGGATGAGAAGCTGGCATCCCTACTGGAAG GGCGCTTCCCACGCAGCACGTCAATGCAGGACACAGTGCGTGAAGGTCGAGGCATTCCACCCCCGCCGCA GACCGCCCCGCCACCCCCACCCGCGCCCTACTACTTCGACTCCGGGCCACCCCCCACCTTCTCACCGCCG CCACCACCGGGCCGGGCCTATGACACTGTGCGCTCCAGCTTCAAACCAGGCCTGGAGGCTCGTCTGGGTG CGGGGGCCGCCGGCCTGTATGATCCGAGCACGCCTCTGGGCCCGCTGCCCTACCCTGAGCGTCAGAAGCG TGCGCGCTCCATGATCATACTGCAGGACTCTGCGCCAGAAGTGGGTGATGTCCCCCGGCCTGCGCCTGCC GCCACACCGCCTGAGCGCCCCAAGCGCCGGCCTCGGCCGTCAGGCCCTGATAGTCCCTATGCCAACCTGG GCGCCTTCAGTGCCAGCCTCTTCGCTCCGTCGAAACCACAGCGCCGCAAGAGCCCGCTGGTGAAGCAGCT TCAGGTGGAGGACGCTCAGGAGCGCGCGGCCCTGGCCGTGGGTAGCCCGGGACCAGTGGGCGGAAGCTTT GCACGAGAACCCTCTCCGACGCACCGCGGGCCCCGGCCAGGCAGCCTTGACTACAGCTCTGGAGAAGGCC TAGGCCTTACCTTCGGCGGCCCTAGCCCTGGCCCAGTCAAGGAGCGGCGCCTGGAGGAGCGACGCCGTTC CACTGTGTTCCTCTCTGTGGGTGCCATCGAGGGCAGCCCTCCCAGCGCGGATCTGCCATCCCTACAACCC TCCCGCTCCATTGATGAGCGCCTCCTGGGGACAGGCGCCACCACTGGCCGCGATTTGCTACTCCCCTCCC CTGTCTCTGCTCTGAAGCCATTGGTCGGTGGTCCCAGCCTTGGGCCCTCAGGCTCCACCTTCATCCACCC TCTCACTGGCAAACCCTTGGATCCTAGCTCACCCTTAGCTCTTGCTCTGGCTGCCCGGGAGCGGGCTCTG GCCTCGCAAACACCTTCCCGGTCCCCCACACCTGTGCACAGCCCCGATGCTGACCGCCCTGGACCCCTCT TTGTGGATGTGCAAACCCGAGACTCTGAGAGAGGACCGTTGGCTTCCCCAGCCTTCTCCCCTCGGAGTCC AGCGTGGATTCCAGTGCCTGCTCGGAGAGAGGCAGAGAAGCCCCCTCGGGAAGAGCGGAAGTCACCAGAG GACAAGAAGTCCATGATCCTCAGCGTCTTGGACACGTCCTTGCAACGGCCAGCTGGCCTCATTGTTGTGC ATGCCACCAGCAATGGGCAGGAGCCCAGCAGGCTGGGGGCTGAAGAGGAGCGCCCCGGTACTCCGGAGCT GGCCCCAGCCCCCATGCAGGCAGCAGCTGTGGCAGAGCCCATGCCAAGCCCCCGGGCCCAGCCCCCTGGC AGCATCCCAGCAGATCCCGGGCCAGGTCAAGGCAGCTCAGAGGAGGAGCCAGAGCTGGTATTCGCTGTGA ACCTGCCACCTGCTCAGCTGTCCTCCAGCGATGAGGAGACCAGAGAGGAGCTGGCCCGCATAGGGCTAGT GCCACCCCCTGAAGAGTTTGCCAATGGGATCCTGCTGACCACCCCGCCCCCAGGGCCGGGCCCCTTGCCC ACCACGGTACCCAGCCCGGCCTCAGGGAAGCCCAGCAGCGAGCTGCCCCCTGCCCCTGAGTCTGCAGCTG ACTCTGGAGTAGAGGAGGCTGACACTCGAAGCTCCAGTGACCCCCACCTGGAGACCACAAGCACCATTTC CACAGTGTCCAGCATGTCCACCCTGAGCTCGGAGAGTGGGGAACTCACGGACACCCACACCTCCTTTGCC GATGGACACACTTTTCTACTCGAGAAGCCACCAGTGCCTCCCAAGCCCAAGCTCAAGTCCCCGCTGGGGA AGGGGCCGGTGACCTTCAGGGACCCGCTGCTGAAGCAATCCTCGGACAGTGAGCTCATGGCCCAGCAGCA CCATGCTGCCTCTACTGGGTTGGCTTCTGCTGCTGGGCCCGCCCGCCCTCGCTACCTCTTCCAGAGAAGG TCCAAGCTGTGGGGGGACCCCGTGGAGAGTCGGGGGCTCCCTGGGCCTGAAGATGACAAACCAACTGTGA TCAGTGAGCTCAGCTCCCGTCTGCAGCAGCTGAATAAAGACACACGCTCCTTGGGGGAGGAACCAGTTGG TGGCCTGGGCAGCCTGCTGGACCCTGCTAAGAAGTCACCCATTGCAGCAGCTCGGCTCTTCAGCAGCCTC GGTGAGCTGAGCACCATCTCAGCGCAGCGCAGCCCGGGGGGCCCGGGCGGAGGGGCCTCCTACTCGGTGC GGCCCAGCGGCCGGTACCCCGTGGCGAGACGAGCCCCGAGCCCAGTGAAACCCGCATCGCTGGAGCGGGT GGAGGGGCTGGGGGCGGGCGTGGGAGGCGCGGGGCGGCCCTTCGGCCTCACGCCTCCCACCATCCTCAAG TCGTCCAGCCTCTCCATCCCGCACGAACCCAAGGAAGTGCGCTTCGTGGTGCGAAGTGTGAGTGCGCGCA GCCGCTCCCCCTCACCATCTCCGCTGCCCTCGCCTTCTCCCGGCTCTGGCCCCAGTGCCGGCCCGCGTCG GCCATTTCAACAGAAGCCCCTGCAGCTCTGGAGCAAGTTCGATGTGGGCGACTGGCTGGAGAGCATCCAC TTAGGCGAGCACCGAGACCGCTTCGAGGACCATGAGATCGAAGGCGCACACCTGCCTGCGCTCACCAAGG AAGACTTCGTGGAGCTGGGCGTCACACGCGTTGGCCACCGCATGAACATCGAGCGTGCGCTCAGGCAGCT GGATGGCAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218960 representing NM_021423 Red=Cloning site Green=Tags(s) MDGPGASAVVVRVGIPDLQQTKCLRLDPTAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDE ERLLQDYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLD PNFHDPDSGECPLSLAAQLDNATDLLKVLRNGGAHLDFRTRDGLTAVHCATRQRNAGALTTLLDLGASPD YKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGTTDENGWQEIHQACRFGHVQHLEHLLFYGANMGAQN ASGNTALHICALYNQESCARVLLFRGANKDVRNYNSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETP SYAKRRRLAGPSGLASPRPLQRSASDINLKGDQPAASPGPTLRSLPHQLLLQRLQEEKDRDRDGELENDI SGPSAGRGGHNKISPSGPGGSGPAPGPGPASPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEIP LHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQYDTRHETREDRTKRLFRHYTVGSYDSLTS HSDYVIDDKVAILQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWRAGLRTGDFLI EVNGVNVVKVGHKQVVGLIRQGGNRLVMKVVSVTRKPEEDGARRRAPPPPKRAPSTTLTLRSKSMTAELE ELASIRRRKGEKLDEILAVAAEPTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLPG SLRKGIPRTKSVGEDEKLASLLEGRFPRSTSMQDTVREGRGIPPPPQTAPPPPPAPYYFDSGPPPTFSPP PPPGRAYDTVRSSFKPGLEARLGAGAAGLYDPSTPLGPLPYPERQKRARSMIILQDSAPEVGDVPRPAPA ATPPERPKRRPRPSGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPVGGSF AREPSPTHRGPRPGSLDYSSGEGLGLTFGGPSPGPVKERRLEERRRSTVFLSVGAIEGSPPSADLPSLQP SRSIDERLLGTGATTGRDLLLPSPVSALKPLVGGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERAL ASQTPSRSPTPVHSPDADRPGPLFVDVQTRDSERGPLASPAFSPRSPAWIPVPARREAEKPPREERKSPE DKKSMILSVLDTSLQRPAGLIVVHATSNGQEPSRLGAEEERPGTPELAPAPMQAAAVAEPMPSPRAQPPG SIPADPGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGILLTTPPPGPGPLP TTVPSPASGKPSSELPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFA DGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHAASTGLASAAGPARPRYLFQRR SKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARLFSSL GELSTISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAGVGGAGRPFGLTPPTILK SSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPSPGSGPSAGPRRPFQQKPLQLWSKFDVGDWLESIH LGEHRDRFEDHEIEGAHLPALTKEDFVELGVTRVGHRMNIERALRQLDGS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021423
ORF Size	5190 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021423.3, NP_067398.2
RefSeq Size	7131 bp
RefSeq ORF	5193 bp
Locus ID	58234
UniProt ID	Q4ACU6
MW	185.4 kDa
Gene Summary	Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.[UniProtKB/Swiss-Prot Function]

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