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Phb2 (NM_007531) Mouse Tagged ORF Clone
CAT＃: MR218376
- TrueORF®
Phb2 (Myc-DDK-tagged) - Mouse prohibitin 2 (Phb2)
ORF Plasmid: tGFP
"NM_007531" in other vectors (4)
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CNY 2,081.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU044498; BAP; Bap37; Bcap37; REA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218376 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAACTTGAAGGACTTAGCTGGACGCCTGCCCGCCGGGCCTCGGGGCATGGGCACGGCGCTGA AGCTGCTGCTGGGGGCCGGGGCGGTGGCCTACGGCGTCCGCGAATCCGTGTTCACCGTGGAAGGCGGTCA TAGAGCCATCTTTTTTAATCGTATTGGTGGCGTGCAGCAGGACACGATCCTGGCCGAAGGCCTTCACTTC AGGATCCCCTGGTTCCAGTACCCCATCATCTATGACATTCGGGCCAGACCTCGAAAAATCTCCTCCCCCA CAGGCTCCAAAGACCTGCAGATGGTGAACATCTCCCTGCGTGTGCTGTCCCGACCCAATGCCCAGGAGCT CCCCAGCATGTACCAGCGTCTAGGGCTGGACTATGAGGAGCGAGTGCTGCCGTCCATTGTTAATGAGGTG CTCAAGAGTGTGGTGGCCAAGTTCAATGCCTCGCAGCTGATCACCCAGCGGGCTCAGGTGTCCCTGTTGA TCCGAAGAGAGCTGACAGAGCGCGCCAAGGACTTCAGCCTCATCCTGGATGATGTAGCTATCACAGAGCT GAGCTTCAGCCGAGAGTACACAGCTGCTGTAGAAGCCAAGCAAGTGGCCCAGCAGGAAGCCCAGCGGGCC CAGTTTTTGGTGGAGAAAGCGAAGCAGGAACAGCGACAGAAGATTGTGCAGGCTGAGGGGGAGGCGGAGG CTGCCAAGATGCTTGGAGAAGCACTGAGCAAGAATCCTGGCTATATCAAGCTCCGAAAGATCCGGGCCGC CCAGAACATCTCTAAAACGATCGCCACATCACAGAACCGAATCTATCTCACAGCTGACAACCTTGTGCTG AATCTACAGGATGAAAGTTTTACTCGGGGAAGTGACAGCCTCATTAAGGGTAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218376 protein sequence
Red=Cloning site Green=Tags(s) MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHF RIPWFQYPIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEV LKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAKQVAQQEAQRA QFLVEKAKQEQRQKIVQAEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADNLVL NLQDESFTRGSDSLIKGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007531 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007531.2, NP_031557.2 |
| RefSeq Size | 1360 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 12034 |
| UniProt ID | O35129 |
| MW | 33.3 kDa |
| Gene Summary | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In mitochondria, regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity (PubMed:20959514).[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagy
,null,
Scientific Reports
,PubMed ID 36593241
[Phb2]
|
|
Prohibitin levels regulate OMA1 activity and turnover in neurons
,Anderson, CJ;Kahl, A;Fruitman, H;Qian, L;Zhou, P;Manfredi, G;Iadecola, C;,
Cell Death Differ.
,PubMed ID 31819158
[PHB2]
|
|
Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation
,Saleh, A;Subramaniam, G;Raychaudhuri, S;Dhawan, J;,
Sci Rep
,PubMed ID 31165762
[PHB2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208177 | Phb2 (untagged) - Mouse prohibitin 2 (Phb2), (10ug) |
CNY 3,990.00 |
|
| MG218376 | Phb2 (tGFP-tagged) - Mouse prohibitin 2 (Phb2), (10ug) |
CNY 2,850.00 |
|
| MR218376L3 | Lenti ORF clone of Phb2 (Myc-DDK-tagged) - Mouse prohibitin 2 (Phb2) |
CNY 4,750.00 |
|
| MR218376L4 | Lenti ORF clone of Phb2 (mGFP-tagged) - Mouse prohibitin 2 (Phb2) |
CNY 4,750.00 |
