Tnks1bp1 (NM_001081260) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA1741; Tab182
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215737 representing NM_001081260 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGATCCACTCTCAGGGAAGGCACAGCCATGGCTTCCCCACTGCCCCAGGATATGGAGGAGGAGC TGGCGCCTGTTGGCTCCGAGCCAGGTGACCCACGAGCCAAGCCCCCTGTCAAGCCCAAACCCCGGGGCTT GCCCAGCAAGCCAGCTCTGCCTGCCAAACCCAGCCTCCTGGTGCCTGTTGGGCCTCGCCCTCCTCGGGGT CCCCTGGCTGAGCTGCCTTCTGCCCGGAAGATGAACATGCTGGCAGGACCTCAGCCCTATGGGGTCAGCA AGCGTCCCCTCCCGTTTGCACCAAGGCCTTCAGCTGAAGCAACCGCTGGAGGAGACGTTACCCAAGAGTC TGGGAAGGAGGATGCTGGGAAAGAAGACTTGCCCCCTCTCACACCCCCAGCTCGGTGCGCTGCCCTGGGA GGTGTGAGGAAGGCCCCTGCCCCTTTCCGTCCCTCCTCTGAGCGGTTTGCAGCCTGTACAGTAGAAGAAA TTTTGGCCAAGATGGAGCAGCCACGGAAAGAGATCCTGGCCAGCCCTGACCGCCTCTGGGGCTCCCGCCT CACTTTTAACCACGATGGCAGCTCTCGATATGGCCCCAGGACCTATGGTGCCCCCTGCCCCAGAGAGGAG GATAGTAAGTCCCCTGCCAAAGGACGGTCCCAGGAGGGGACAGCAGAGATTCCTGCAGAGTGCCAGGAAG AGCATAGCAAGACTCCTGAGGAGAGGAACCTCACTTCCAGCCCGGCAATGAATGGGGACCTAGCTAAACT GGCTTGCTCTGAAGCACCTACCGACGTTTCCAAGACCTGGGTCACCTCAAGTGCAGACCCTGTTTCAGAG CATGGCGGCTCTACCAGTGCAGTCCGCCTGGCTAACATCTCTGTCCCAGCCTCGGAGTCTCCCCGGCTTT CAAGCCGCCCGAGTTCTCCCTGCCATTCTCAACTTTCAGAAACCCAAAGTCCTGCAGCTTCTGAGGCTTC TTCCATCTGCCTCCCTGTGACTCCAGCCTCCCCCAGTGCAGTCTTGCCTGCTGAGCCTCCCGGCCATTCT CCTAGCTCAGAGTTACCTGCTGAGGCAGCCCCAGAAACCCTCAGCCCCAACAGCTCTCCTGTGGAGACAG TCTCAGGGCACCACAGCCCAGAGCAGCCCCCAGTCCTCTTGCCTCAACTCCTGACTGAGGGGGCTGAGTT GCCGGACATTACACGAACGTTCCCCTGTGGGGAGGAGGCAGCAGCCAGGGGTCACACGGAATCTCGTCCC AGCAGCCTGGCTCAGCGTCGTTTCTCAGAAGGCGTGCTCCAGCCACCTAGCCAGGACCAAGAGAAGCTGG GAGGCTCGCTGGCTACTCTGCCCCAAGGTCAGGGGAGCCAATCTGCTTTAGATCGTCCCTTCGGAAGCGG GACAGAGTCTAATTGGAGCTTGTCACAGTCATTTGAGTGGACCTTTCCCACAAGGCCCTCAGGTCTGGGC GTGTGGCGGCTGGACTCCCCACCTCCATCTCCCATTACAGAAGCCAGTGAGGCCGCAGAGGCTGCTGAAG CTGACAGTTGGGCTGTATCTGGCAGGGGGGAAGGTGTGTCCCAGGTGGGACCAGGAACCCCACCAGCTCC AGAGAGCCCCAGAAAGCCTATTTCTGGGGTTCAGGGAAATGATCCAGGCATATCCCTACCCCAGAGAGAT GATGGAGAGAGTCAGCCTCGGTCCCCAGCTCTTCTTCCCTCTACAGTTGAGGGCCCACCTGGAGCACCCT TACTGCAGGCAAAGGAGAACTATGAGGACCAGGAGCCACTAGTGGGGCACGAGTCCCCCATTACTTTGGC TGCAAGGGAGGCGGCCTTACCTGTGCTAGAGCCAGCGCTGGGACAGCAGCAGCCAACACCCTCTGACCAA CCCTGCATCCTCTTTGTGGATGTGCCCGATCCTGAGCAGGCATTGTCTACTGAGGAGGACGTGGTGACCC TAGGCTGGGCGGAGACGACCCTGCCCATGACAGAGGCTCAGGAACCCTGTAGTGTGTCTCCTGAGCCTAC AGGCCCTGAAAGCAGTTCTCGATGGCTGGATGACCTCCTAGCATCACCACCACCCAACTCGGGCAGTGCG CGGCGGGCAGCTGGAGCTGAGCTAAAGGATAGACAGTCACCAAGCACCTGCTCTGAGGGACTCCTTGGCT GGGCCCAGAAAGACCTGCAGAGTGAATTTGGGGTTGCCACAGACTCACACCACAGCAGTTTTGGTTCTTC TAGCTGGTCTCAAGACACTTCTCAGAACTATAGCCTCGGGGGCAGGAGCCCCGTTGGAGACACAGGTCTT GGGAAAAGAGACTGGAGCAGCAAGTGTGGGCAAGGGTCCGGGGAGGGGAGCACTAGGGAGTGGGCCAGCA GACACAGCCTTGGCCAAGAGGTGATTGGAATCGGTGGCAGCCAAGACGAGAGCGAAGTGCCTGTCCGTGA GCGGGCAGTGGGAAGGCCGGCCCAGCTTGGTGCTCAGGGCCTGGAGGCTGATGCACAGCAATGGGAATTC GGGAAGAGGGAGTCCCAGGACCCTCACTCCATCCACGACAAAGAGCTCCAGGACCAGGAGTTCGGGAAGC GAGATTCCTTGGGTTCTTTCAGCACCCGAGATGCAAGCCTTCAAGACTGGGAGTTTGGGAAGAGAGCGTC TGTGAGCACCAACCAGGATACAGATGAGAATGATCAAGAACTGGGGATGAAGAACCTCTCTCGTGGCTAC AGCAGCCAGGATGCTGAAGAGCAGGACCGGGAGTTTGAGAAGCGAGATTCTGTACTGGACATCCATGGCA GCCGGGCCACAGCCCAGCAGAACCAGGAGTTCGGGAAGAGTGCTTGGTTCCAGGATTACAGTAGTGGTGG TGGCGGCTCAAGGGTGCTCGGCTCACAGGAGAGAGGTTTCGGAATTCGATCTCTGAGCTCAGGGTTCAGC CCCGAGGAAGCTCAGCAGCAAGATGAGGAGTTTGAGAAGAAGACCCCAGTTGGTGAAGACAGGTTTTGTG AGGCCAGCAGGGATGTGGGTCACCTGGAAGAAGGAGCATCTGGGGGCCTGCTGAGCCCCAGCACACCTCA TTCTCGAGATGGAGCTGCCAGGCCAAAAGACGAAGGCAGCTGGCAAGATGGTGACTCCAGCCAGGAGATC ACAAGGCTGCAGGGGAGAATGCAGGCAGAAAGTCAGAGCCCTACCAACGTGGACCTGGAAGACAAAGAGA GAGAGCAGAGAGGCTGGGCCGGGGAGTTCAGCCTCGGTGTTGCAGCCCAGTCGGAGGCAGCATTTAGTCC AGGGCGGCAAGACTGGAGCAGGGACGTGTGTGTGGAGGCCTCTGAGAGTAGCTATCAGTTTGGCATCATC GGCAATGACAGAGTGAGTGGTGCTGGCCTCAGCCCTTCCAGGAAGAGTGGAGGTGGCCACTTTGTGCCTC CCGGAGAGACAAAGGCCGGGGCTGTGGATTGGACTGACCAGCTAGGTCTCAGGAACTTGGAGGTGTCTAG CTGTGTGAGTTCTGAAGGCCCAAGCGAGGCTAGAGAGAATGTTGTAGGACAGATGGGCTGGTCAGACAGC CTGGGCTTGAACAATGGAGATCTGGCCAGGCGTTTAGGAACTGGAGAGTCTGAGGAGCCCAGGAGCCTGG GAGTTGGGGAAAAGGACTGGACTTCCAGCGTGGAGGCGAGGAACAGAGATTTGCCAGGGCAGGCAGAGGT AGGAAGGCACAGCCAGGCCAGAGAGAGTGGTGTGGGGGAGCCCGACTGGTCAGGGGCAGAGGCCGGAGAG TTCCTTAAGTCAAGAGAGCGTGGAGTTGGACAGGCAGATTGGACACCTGATCTTGGATTGAGAAACATGG CCCCAGGAGCAGGCTGCAGCCCTGGAGAGCCCAGGGAACTTGGGGTGGGTCAGGTAGACTGGGGTGACGA TCTAGGCCTGAGGAATTTGGAGGTGTCCTGTGACCTAGAGTCGGGAGGCTCTCGGGGCTGTGGAGTTGGG CAAATGGACTGGGCCCAAGACTTGGGACTCCGGAACCTTAGGCTCTGTGGGGCCCCAAGTGAAGTTAGGG AATGTGGGGTGGGGAGAGTGGGCCCTGACCTAGAGCTAGATCCCAAGAGTAGTGGTAGCTTGTCCCCTGG TTTGGAGACTGAAGACCCGTTGGAAGCCAGGGAGCTGGGGGTTGGTGAAATAAGTGGGCCAGAGACACAG GGTGAAGACAGCTCGTCACCTTCCTTTGAGACTCCTTCTGAAGACACTGGAATGGATACGGGAGAAGCAC CCAGCTTGGGAGCCAGCCCCAGCAGTTGTCTGACTCGCTCGCCACCTTCTGGCTCCCAGAGCCTTTTGGA GGGGATAATGACAGCCAGCAGCTCTAAGGGAGCTCCTCAGAGGGAATCAGCAGCTTCCGGCTCCCGGGTT CTGTTGGAGGAGGAAGGACTCGCTGCAGGTGCCGGCCAAGGGGAGCCCCAGGAGCCTAGCAGAGCTCCTT TGCCATCCTCGAGGCCTCAGCCTGATGGCGAAGCCAGCCAGGTAGAAGAGGTGGATGGCACGTGGAGTCT TACGGGGGCGGCTAGGCAAAATGAGCAGGCATCAGCCCCGCCCCCCCGGCGGCCTCCCCGAGGACTTCTG CCCAGCTGCCCCAGTGAGGACTTCTCTTTTATAGAGGACACTGAGATCCTTGATAGTGCCATGTATCGGA GTCGTGCCAACCTAGGACGTAAGCGGGGGCACCGGGCCCCAGCCATCCGGCCTGGGGGTACCCTGGGACT GTCAGAGACAGCAGACTCGGATACTCGGCTGTTCCAAGATTCAACCGAGCCAAGGGCCTCCCGGGTGCCA TCTTCAGATGAGGAGGTTGTAGAGGAGCCTCAGAGCCGCCGGACACGGATGTCCTTGGGCACTAAAGGGC TGAAAGTCAACCTCTTTCCTGGCCTGAGCCCATCAGCCTTGAAGGCCAAGCTACGCTCCCGGAACCGCTC AGCTGAGGAGGGGGAGGTGACAGAGAGCAAATCAAGCCAGAAAGAGTCTTCCGTCCAGCGTTCTAAGTCC TGCAAGGTCCCAGGGCTAGGGAAGCCCCTTACATTACCCCCAAAGCCAGAAAAATCCTCAGGGTCAGAAG GATCGTCGCCCAACTGGCTGCAAGCCCTGAAGCTAAAAAAGAAAAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215737 representing NM_001081260 Red=Cloning site Green=Tags(s) MKGSTLREGTAMASPLPQDMEEELAPVGSEPGDPRAKPPVKPKPRGLPSKPALPAKPSLLVPVGPRPPRG PLAELPSARKMNMLAGPQPYGVSKRPLPFAPRPSAEATAGGDVTQESGKEDAGKEDLPPLTPPARCAALG GVRKAPAPFRPSSERFAACTVEEILAKMEQPRKEILASPDRLWGSRLTFNHDGSSRYGPRTYGAPCPREE DSKSPAKGRSQEGTAEIPAECQEEHSKTPEERNLTSSPAMNGDLAKLACSEAPTDVSKTWVTSSADPVSE HGGSTSAVRLANISVPASESPRLSSRPSSPCHSQLSETQSPAASEASSICLPVTPASPSAVLPAEPPGHS PSSELPAEAAPETLSPNSSPVETVSGHHSPEQPPVLLPQLLTEGAELPDITRTFPCGEEAAARGHTESRP SSLAQRRFSEGVLQPPSQDQEKLGGSLATLPQGQGSQSALDRPFGSGTESNWSLSQSFEWTFPTRPSGLG VWRLDSPPPSPITEASEAAEAAEADSWAVSGRGEGVSQVGPGTPPAPESPRKPISGVQGNDPGISLPQRD DGESQPRSPALLPSTVEGPPGAPLLQAKENYEDQEPLVGHESPITLAAREAALPVLEPALGQQQPTPSDQ PCILFVDVPDPEQALSTEEDVVTLGWAETTLPMTEAQEPCSVSPEPTGPESSSRWLDDLLASPPPNSGSA RRAAGAELKDRQSPSTCSEGLLGWAQKDLQSEFGVATDSHHSSFGSSSWSQDTSQNYSLGGRSPVGDTGL GKRDWSSKCGQGSGEGSTREWASRHSLGQEVIGIGGSQDESEVPVRERAVGRPAQLGAQGLEADAQQWEF GKRESQDPHSIHDKELQDQEFGKRDSLGSFSTRDASLQDWEFGKRASVSTNQDTDENDQELGMKNLSRGY SSQDAEEQDREFEKRDSVLDIHGSRATAQQNQEFGKSAWFQDYSSGGGGSRVLGSQERGFGIRSLSSGFS PEEAQQQDEEFEKKTPVGEDRFCEASRDVGHLEEGASGGLLSPSTPHSRDGAARPKDEGSWQDGDSSQEI TRLQGRMQAESQSPTNVDLEDKEREQRGWAGEFSLGVAAQSEAAFSPGRQDWSRDVCVEASESSYQFGII GNDRVSGAGLSPSRKSGGGHFVPPGETKAGAVDWTDQLGLRNLEVSSCVSSEGPSEARENVVGQMGWSDS LGLNNGDLARRLGTGESEEPRSLGVGEKDWTSSVEARNRDLPGQAEVGRHSQARESGVGEPDWSGAEAGE FLKSRERGVGQADWTPDLGLRNMAPGAGCSPGEPRELGVGQVDWGDDLGLRNLEVSCDLESGGSRGCGVG QMDWAQDLGLRNLRLCGAPSEVRECGVGRVGPDLELDPKSSGSLSPGLETEDPLEARELGVGEISGPETQ GEDSSSPSFETPSEDTGMDTGEAPSLGASPSSCLTRSPPSGSQSLLEGIMTASSSKGAPQRESAASGSRV LLEEEGLAAGAGQGEPQEPSRAPLPSSRPQPDGEASQVEEVDGTWSLTGAARQNEQASAPPPRRPPRGLL PSCPSEDFSFIEDTEILDSAMYRSRANLGRKRGHRAPAIRPGGTLGLSETADSDTRLFQDSTEPRASRVP SSDEEVVEEPQSRRTRMSLGTKGLKVNLFPGLSPSALKAKLRSRNRSAEEGEVTESKSSQKESSVQRSKS CKVPGLGKPLTLPPKPEKSSGSEGSSPNWLQALKLKKKKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081260
ORF Size	5160 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081260.2, NP_001074729.1
RefSeq Size	5781 bp
RefSeq ORF	5163 bp
Locus ID	228140
UniProt ID	P58871
MW	182.3 kDa

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