Cavin4 (NM_026509) Mouse Tagged ORF Clone

CAT#: MR215243

  • TrueORF®

Murc (Myc-DDK-tagged) - Mouse muscle-related coiled-coil protein (Murc)

ORF Plasmid: DDK tGFP



  "NM_026509" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2310039E09Rik; Murc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR215243 representing NM_026509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACACAACGGATCAGCTTCAAATGCTGGTAAAATCCACCAGAATCGGTTGTCAAGTGTGACGGAAG
ATGAAGACCAAGACGCAGCCCTCACCATTGTGACCGTGCTGGACAGAGTGGCCAGTGTCGTGGACAGTGT
GCAGGCAAGCCAGAAGAGAATTGAGGAGAGACACAGAGAAATGGGTAACGCGATAAAGTCTGTCCAAATA
GACCTGCTGAAGCTCTCACAGTCACACAGCAATACGGGCTACGTTGTCAACAAGCTGTTTGAGAAGACCC
GGAAAGTCAGCGCTCACATTAAGGATGTCAAGGCCCGGGTAGAGAAGCAACAGGTTCGTGTAACCAAAGT
CGAAACCAAGCAAGAAGAAATAATGAAGAAAAACAAATTCCGAGTGGTAATCTTCCAGGAGGACATTCCC
TGCCCCGCATCCCTGTCTGTTGTTAAAGACAGAAGCCTGCCCGAGAACCAGGAGGAGGCCGAAGAAGTCT
TCGATCCCCCAATAGAGCTCTCCTCGGATGAGGAGTATTATGTTGAAGAAAGCAGATCTGCCAGGCTTAG
GAAGTCAGGCAAAGAGCACATTGACCACATCAAGAAAGCCTTTTCCAGAGAAAACATGCAGAAGACACGG
CAGACTCTTGACAAGAAAGTGAGTGGGATTAGAACTAGGATAGTCACTCCTGAGAGGAGAGAGAGGCTGA
GGCAGTCGGGAGAGAGGCTGAGGCAGTCAGGAGAGAGGCTGAGGCAGTCGGGGGAAAGATTTAAGAAATC
GATTTCCAGTGCCGCTCCCTCAAAGGAAGCTTTTAAGATTCGGAGCCTTAGGAAAGCGAAGGATCCCAAG
GCCGAGGGCCAGGAGGTAGACAGAGGGATGGGCGTGGACATCATCTCAGGTAGCCTGGCTCTGGGGCCCA
TCCATGAGTTCCACTCTGATGAGTTCAGTGAAACAGAAAAGGAGGTGACCAAAGGAGGGTACAGTCCCCA
AGAAGGAGGGGACCCCCCAACGCCTGAGCCTTTGAAGGTGACTTTTAAACCCCAGGTGAGGGTAGAGGAT
GACGAGTCACTCCTGTTGGAGCTAAAGCAGTCCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR215243 representing NM_026509
Red=Cloning site Green=Tags(s)

MEHNGSASNAGKIHQNRLSSVTEDEDQDAALTIVTVLDRVASVVDSVQASQKRIEERHREMGNAIKSVQI
DLLKLSQSHSNTGYVVNKLFEKTRKVSAHIKDVKARVEKQQVRVTKVETKQEEIMKKNKFRVVIFQEDIP
CPASLSVVKDRSLPENQEEAEEVFDPPIELSSDEEYYVEESRSARLRKSGKEHIDHIKKAFSRENMQKTR
QTLDKKVSGIRTRIVTPERRERLRQSGERLRQSGERLRQSGERFKKSISSAAPSKEAFKIRSLRKAKDPK
AEGQEVDRGMGVDIISGSLALGPIHEFHSDEFSETEKEVTKGGYSPQEGGDPPTPEPLKVTFKPQVRVED
DESLLLELKQSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026509
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_026509.3, NP_080785.2
RefSeq Size 2062 bp
RefSeq ORF 1089 bp
Locus ID 68016
UniProt ID A2AMM0
MW 41.5 kDa
Gene Summary Modulates the morphology of formed caveolae in cardiomyocytes, but is not required for caveolar formation. Facilitates the recruitment of MAPK1/3 to caveolae within cardiomyocytes and regulates alpha-1 adrenergic receptor-induced hypertrophic responses in cardiomyocytes through MAPK1/3 activation (PubMed:24567387). Contributes to proper membrane localization and stabilization of caveolin-3 (CAV3) in cardiomyocytes (PubMed:26497963). Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway (PubMed:18332105).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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