Plxna2 (NM_008882) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2810428A13Rik; AA589422; AW457381; mKIAA0463; OCT; PlexA2; Plxn2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212094 representing NM_008882 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGAGGAGGTTCTATCTGCGGGCCATGCAGGCTGACAACCTGTCTGTGGTTCTGCTCTCAGTGG CCTGGCTCCTGCTAGCTCGTGGGACCACAGGTATGCCTCAGTACAGCACTTTCCACTCTGAGAATCGTGA CTGGACTTTCAACCATTTGACTGTACACCGAAGAACAGGGGCTGTGTATGTGGGGGCTATCAATCGTGTC TACAAGTTGACTGGCAACCTCACCATCCAGGTGGCTCACAAGACAGGGCCAGAAGAGGACAACAAGGCTT GCTACCCACCCCTCATTGTACAGCCCTGCAGTGAAGTGCTTACACTCACCAACAATGTCAACAAACTACT GATCATTGACTACTCTGAGAATCGCCTGCTGGCCTGCGGAAGCCTCTACCAGGGTGTTTGCAAGCTCCTG CGACTAGATGACCTCTTCATCCTGGTGGAGCCATCCCACAAGAAGGAACACTACTTGTCCAGTGTCAATA AGACAGGCACCATGTATGGTGTGATTGTGCGCTCTGAGGGGGAAGATGGCAAGCTTTTTATCGGCACTGC TGTGGATGGCAAGCAGGATTACTTCCCTACTCTGTCCAGCCGCAAGCTGCCCCGTGACCCTGAGTCTTCA GCAATGCTGGACTATGAGCTCCACAGTGATTTTGTCTCCTCCCTCATCAAGATTCCCTCTGACACCCTAG CCCTGGTCTCTCACTTCGACATCTTCTACATCTATGGCTTTGCCAGTGGGGGGTTTGTCTACTTTCTCAC TGTCCAGCCAGAGACCCCTGACGGCATGGCCATCAATTCAGCTGGAGACCTCTTCTATACCTCAAGAATT GTGCGTCTCTGCAAGGATGACCCCAAGTTCCACTCCTATGTGTCCCTGCCTTTTGGCTGCACACGTGCTG GGGTGGAATATCGCCTTCTGCAGGCAGCTTACCTTGCAAAGCCAGGGGAAGCTCTAGCTCAGGCCTTCAA CATCAGCAGCGACGAAGATGTCCTGTTTGCCATCTTCTCCAAGGGGCAGAAGCAGTACCACCACCCCCCT GATGACTCTGCCCTCTGTGCCTTCCCCATCCGGGCCATCAACTTGCAAATCAAGGAGCGGTTGCAGTCCT GCTACCATGGAGAGGGCAACTTGGAGCTCAACTGGCTGCTGGGAAAGGATGTGCAGTGCACCAAGGCGCC TGTCCCAATCGATGATAACTTCTGCGGCCTGGACATCAACCAGCCTCTGGGAGGCTCCACTCCTGTGGAG GGACTGACCCTGTATACCACCAGCAGGGACCGCCTGACCTCTGTGGCCTCCTATGTTTACAATGGCTACA GTGTGGTTTTTGTGGGGACTAAGAGTGGCAAGCTGAAGAAGATTCGAGCTGATGGTCCCCCCCATGGTGG GGTCCAGTATGAGATGGTCTCTGTGTTCAAAGATGGGAGCCCAATCCTCCGGGACATGGCCTTCTCCATC AATCAGCTATACCTATATGTCATGTCTGAGAGACAGGTCACCAGGGTCCCTGTTGAATCATGTGAACAGT ATACAACTTGTGGAGAGTGTCTAAGCTCAGGGGATCCTCACTGTGGCTGGTGTGCCCTGCACAACATGTG CTCCCGAAGAGACAAATGCCAACGGGCCTGGGAAGCAAATCGATTTGCTGCCAGTATCAGCCAGTGCATG AGCCTTGAGGTACACCCCAACAGCATCTCTGTGTCAGATCACAGCCGGCTGCTCAGCCTGGTTGTGAATG ATGCTCCCAACCTCTCTGAAGGTATTGCTTGTGCCTTTGGGAATCTGACTGAGGTGGAGGGACAGGTATC TGGGAGTCAAGTCATCTGCATCTCACCTGGACCCAAGGATGTCCCTGTCATCCCTCTGGATCAAGACTGG TTTGGCCTAGAGCTGCAGCTGAGATCCAAAGAGACAGGAAAGATCTTTGTCAGCACCGAATTCAAGTTCT ATAACTGCAGTGCCCACCAACTGTGCCTGTCCTGTGTTAACAGCGCCTTCCGCTGCCATTGGTGCAAGTA CCGTAACCTCTGCACACATGACCCCACTACCTGTTCCTTCCAGGAAGGCAGGATCAATGTTTCAGAGGAC TGTCCCCAGCTCGTGCCCACGGAGGAGATTCTGATCCCAGTTGGGGAAGTAAAACCAATCACCCTTAAGG CCCGAAACCTGCCCCAGCCCCAGTCTGGCCAGCGAGGCTACGAGTGTGTGCTCAGCATTCAAGGGGCTGT CCACCGGGTCCCTGCCCTGCGTTTCAACAGTTCCAGTGTGCAGTGCCAAAACAGCTCGTACCAGTATGAT GGGATGGACATCAGCAACCTAGCAGTGGACTTTGCTGTAGTATGGAATGGCAACTTCATTATTGACAACC CTCAGGACCTGAAAGTACATCTCTACAAGTGTGCAGCCCAGCGGGAAAGCTGTGGTCTCTGCCTCAAGGC TGACCACAAGTTCGAGTGTGGCTGGTGCAGTGGTGAGCGCAGATGTACCCTCCACCAGCACTGCCCCAGC ACTTCTAGCCCCTGGCTTGACTGGTCCAGCCACAATGTCAAGTGTTCCAACCCCCAAATCACAGAGATTT TGACAGTATCAGGACCACCTGAAGGAGGGACTCGTGTGACCATCCATGGCGTGAACCTGGGCTTGGACTT CTCTGAGATTGCTCACCATGTGCAGGTGGCTGGAGTGCCCTGCACACCTATCCCAGGGGAATACATCATC GCTGAGCAGATCGTCTGTGAGATGGGCCATGCCGTTATAGGTACCACATCTGGGCCTGTGCGCCTGTGCA TTGGGGAATGCAAGCCAGAGTTCATGACCAAGTCCCACCAGCAGTATACTTTTGTGAATCCTTCTGTGCT GTCACTCAGCCCGATCCGGGGACCAGAGTCAGGAGGTACCATGGTGACCATCACAGGCCATTACCTTGGT GCTGGGAGCAGTGTGGCAGTGTACCTGGGCAATCAGACCTGTGAATTCTATGGGAGGTCAATGAATGAGA TTGTATGTGTTTCACCCCCATCATCCAATGGACTAGGACCAGTCCCTGTCTCCGTGAGTGTCGACAGAGC CCGGGTGGATAGCAGTCTGCAGTTCGAGTATATAGATGACCCACGGGTCCAACGTATTGAGCCAGAGTGG AGTATCACTAGTGGGCACACACCCCTAACCATCACAGGCTTCAACTTGGATGTCATTCAGGAGCCCAGGG TCCGAGTCAAATTTAATGGCAAAGAATCTGTCAATGTATGCACAGTGGTAAACACAACCACCCTCACCTG TCTGGCACCCTCTCTGACCAGTGACTACCGCCCAGGTCTGGACACTGTGGAACGGCCAGATGAGTTTGGA TTTCTCTTTAACAATGTTCAATCCTTACTCATCTATAACGACACCAAGTTCATCTACTACCCCAACCCAA CGTTTGAACTGCTCAGCCCCACTGGAATCTTGGATCAGAAGCCAGGCTCACCCATCATCCTGAAGGGCAA AAATCTCTGTCCTCCTGCCTCTGGAGGGGCCAAACTCAACTACACAGTAATGATTGGAGAGACACCTTGT ACAGTCACTGTGTCTGAGACACAGCTGCTTTGTGAACCTCCCAACCTCACAGGGCAGCACAAGGTCATGG TTCACGTGGGCGGGATGGTGTTCTCACCTGGCTCCGTGAGCGTCATCTCCGACAGCCTGTTGACCCTGCC AGCCATCATCAGCATCGCAGCTGGTGGAAGCCTCCTTCTTATCATCGTCATCATTGTCCTCATCGCTTAC AAGCGCAAGTCTAGGGAAAATGACCTCACACTCAAGCGGCTCCAAATGCAAATGGACAACCTGGAGTCCA GGGTGGCACTGGAGTGCAAGGAAGCTTTTGCGGAGCTTCAGACAGACATCAATGAGCTAACCAGTGACTT GGATCGATCAGGAATCCCTTACCTGGACTACCGTACCTATGCCATGAGAGTCCTGTTCCCAGGCATTGAG GACCACCCTGTTCTGCGGGAACTGGAGGTACAGGGAAATGGACAGCAGCACGTGGAGAAAGCCCTGAAAC TCTTCGCCCAGCTTATCAACAACAAGGTGTTCTTGCTGACCTTCATCCGTACACTGGAACTACAGCGCAG CTTCTCCATGCGAGACCGTGGGAACGTGGCCTCTCTCATCATGACAGGCCTTCAGGGTCGCCTAGAATAT GCCACTGATGTCCTCAAGCAGCTGCTCTCTGACCTCATTGACAAGAACCTGGAGAACAAGAACCACCCCA AGCTGCTTCTCCGCAGGACTGAGTCTGTGGCCGAGAAGATGCTGACTAACTGGTTTGCTTTTCTTCTACA CAAGTTCCTGAAGGAGTGTGCTGGGGAACCACTCTTCATGCTATACTGTGCAATCAAGCAGCAGATGGAA AAAGGCCCCATTGACGCTATTACTGGTGAGGCCCGATACTCCCTGAGTGAAGACAAGCTCATCCGGCAGC AGATCGAGTATAAGACTCTGATCCTGAACTGTGTCAACCCTGACAATGAGAACAGCCCAGAGATCCCAGT GAAAGTACTAAACTGTGACACCATCACTCAAGTCAAGGAGAAGATCCTCGATGCCGTATATAAGAATGTC CCCTACTCCCAGCGGCCAAGGGCTGTGGACATGGATCTGGAGTGGCGCCAAGGCCGGATTGCCAGAGTGG TGTTGCAGGACGAAGACATTACCACCAAAATAGAGGGTGACTGGAAGCGGCTTAACACACTGATGCATTA CCAGGTGTCAGACAGATCCGTGGTGGCTCTGGTTCCTAAGCAGACCTCCTCCTACAACATCCCTGCCTCT GCCAGCATCTCTCGGACATCCATTAGCAGATATGACTCTTCCTTCAGGTACACAGGCAGCCCAGACAGCC TCCGGTCCCGGGTCCCCATGATCACCCCAGACTTGGAGAGCGGTGTCAAGGTTTGGCATCTGGTGAAGAA TCATGACCATGGTGACCAGAAGGAGGGTGACCGGGGCAGCAAAATGGTGTCTGAGATCTACTTGACCCGG CTTCTAGCCACCAAGGGCACCCTGCAGAAATTTGTGGACGACTTGTTTGAGACCTTGTTCAGCACTGTGC ACCGGGGTAGTGCTCTCCCCCTAGCCATCAAGTACATGTTTGATTTCCTGGATGAGCAGGCAGACAGACA CAGTATCCACGACACAGATGTGCGGCACACCTGGAAAAGCAACTGCCTTCCACTTCGTTTCTGGGTGAAT GTCATCAAGAACCCTCAATTTGTATTTGACATCCACAAGGGCAGCATCACAGATGCCTGCCTCTCTGTGG TAGCCCAGACCTTTATGGACTCCTGTTCCACATCAGAGCACCGACTAGGCAAGGACTCACCTTCCAACAA GCTGCTCTATGCCAAGGATATCCCCAGTTATAAGAACTGGGTAGAAAGATACTATGCAGATATTGCCAAG CTCCCAGCCATTAGTGACCAAGATATGAATGCCTACCTCGCGGAGCAGTCCCGCCTGCATGCTACAGAGT TCAATATGCTGAGCGCCCTCAACGAGATCTACTCATATGTCAGCAAGTACAGTGAGGAGCTCATCGGGGC ACTAGAGCAGGATGAACAGGCCCGACGGCAACGACTGGCCTACAAGGTGGAGCATCTCATCAACGCCATG TCCATAGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212094 representing NM_008882 Red=Cloning site Green=Tags(s) MEQRRFYLRAMQADNLSVVLLSVAWLLLARGTTGMPQYSTFHSENRDWTFNHLTVHRRTGAVYVGAINRV YKLTGNLTIQVAHKTGPEEDNKACYPPLIVQPCSEVLTLTNNVNKLLIIDYSENRLLACGSLYQGVCKLL RLDDLFILVEPSHKKEHYLSSVNKTGTMYGVIVRSEGEDGKLFIGTAVDGKQDYFPTLSSRKLPRDPESS AMLDYELHSDFVSSLIKIPSDTLALVSHFDIFYIYGFASGGFVYFLTVQPETPDGMAINSAGDLFYTSRI VRLCKDDPKFHSYVSLPFGCTRAGVEYRLLQAAYLAKPGEALAQAFNISSDEDVLFAIFSKGQKQYHHPP DDSALCAFPIRAINLQIKERLQSCYHGEGNLELNWLLGKDVQCTKAPVPIDDNFCGLDINQPLGGSTPVE GLTLYTTSRDRLTSVASYVYNGYSVVFVGTKSGKLKKIRADGPPHGGVQYEMVSVFKDGSPILRDMAFSI NQLYLYVMSERQVTRVPVESCEQYTTCGECLSSGDPHCGWCALHNMCSRRDKCQRAWEANRFAASISQCM SLEVHPNSISVSDHSRLLSLVVNDAPNLSEGIACAFGNLTEVEGQVSGSQVICISPGPKDVPVIPLDQDW FGLELQLRSKETGKIFVSTEFKFYNCSAHQLCLSCVNSAFRCHWCKYRNLCTHDPTTCSFQEGRINVSED CPQLVPTEEILIPVGEVKPITLKARNLPQPQSGQRGYECVLSIQGAVHRVPALRFNSSSVQCQNSSYQYD GMDISNLAVDFAVVWNGNFIIDNPQDLKVHLYKCAAQRESCGLCLKADHKFECGWCSGERRCTLHQHCPS TSSPWLDWSSHNVKCSNPQITEILTVSGPPEGGTRVTIHGVNLGLDFSEIAHHVQVAGVPCTPIPGEYII AEQIVCEMGHAVIGTTSGPVRLCIGECKPEFMTKSHQQYTFVNPSVLSLSPIRGPESGGTMVTITGHYLG AGSSVAVYLGNQTCEFYGRSMNEIVCVSPPSSNGLGPVPVSVSVDRARVDSSLQFEYIDDPRVQRIEPEW SITSGHTPLTITGFNLDVIQEPRVRVKFNGKESVNVCTVVNTTTLTCLAPSLTSDYRPGLDTVERPDEFG FLFNNVQSLLIYNDTKFIYYPNPTFELLSPTGILDQKPGSPIILKGKNLCPPASGGAKLNYTVMIGETPC TVTVSETQLLCEPPNLTGQHKVMVHVGGMVFSPGSVSVISDSLLTLPAIISIAAGGSLLLIIVIIVLIAY KRKSRENDLTLKRLQMQMDNLESRVALECKEAFAELQTDINELTSDLDRSGIPYLDYRTYAMRVLFPGIE DHPVLRELEVQGNGQQHVEKALKLFAQLINNKVFLLTFIRTLELQRSFSMRDRGNVASLIMTGLQGRLEY ATDVLKQLLSDLIDKNLENKNHPKLLLRRTESVAEKMLTNWFAFLLHKFLKECAGEPLFMLYCAIKQQME KGPIDAITGEARYSLSEDKLIRQQIEYKTLILNCVNPDNENSPEIPVKVLNCDTITQVKEKILDAVYKNV PYSQRPRAVDMDLEWRQGRIARVVLQDEDITTKIEGDWKRLNTLMHYQVSDRSVVALVPKQTSSYNIPAS ASISRTSISRYDSSFRYTGSPDSLRSRVPMITPDLESGVKVWHLVKNHDHGDQKEGDRGSKMVSEIYLTR LLATKGTLQKFVDDLFETLFSTVHRGSALPLAIKYMFDFLDEQADRHSIHDTDVRHTWKSNCLPLRFWVN VIKNPQFVFDIHKGSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKNWVERYYADIAK LPAISDQDMNAYLAEQSRLHATEFNMLSALNEIYSYVSKYSEELIGALEQDEQARRQRLAYKVEHLINAM SIES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008882
ORF Size	5682 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008882.2, NP_032908.2
RefSeq Size	11049 bp
RefSeq ORF	5685 bp
Locus ID	18845
UniProt ID	P70207
MW	212 kDa
Gene Summary	Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.[UniProtKB/Swiss-Prot Function]

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