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Srebf1 (NM_011480) Mouse Tagged ORF Clone
CAT＃: MR211688
- TrueORF®
Srebf1 (Myc-DDK-tagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1)
"NM_011480" in other vectors (3)
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CNY 11,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADD-; ADD1; bHLHd; bHLHd1; SRE; SREB; SREBP; SREBP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211688 representing NM_011480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGCTGGCCTTCGGTGAGGCGGCTCTGGAACAGACACTGGCCGAGATGTGCGAACTGGACACAG CGGTTTTGAACGACATCGAAGACATGCTCCAGCTCATCAACAACCAAGACAGTGACTTCCCGGGCCTGTT TGACGCCCCCTATGCTGGGGGTGAGACAGGGGACACAGGCCCCAGCAGCCCAGGTGCCAACTCTCCTGAG AGCTTCTCTTCTGCTTCTCTGGCCTCCTCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTGCAC CCTTGTCCCCTCCACCATCGGCACCCGCTGCTTTAAAGATGTACCCGTCCGTGTCCCCCTTTTCCCCTGG GCCTGGGATCAAAGAGGAGCCAGTGCCACTCACCATCCTACAGCCTGCAGCGCCACAGCCGTCACCGGGG ACCCTCCTGCCTCCGAGCTTCCCCGCACCACCCGTACAGCTCAGCCCTGCGCCCGTGCTGGGTTACTCGA GCCTGCCTTCAGGCTTCTCAGGGACCCTTCCAGGAAACACTCAGCAGCCACCATCTAGCCTGCCGCTGGC CCCTGCACCAGGAGTCTTGCCCACCCCTGCCCTGCACACCCAGGTCCAAAGCTTGGCCTCCCAGCAGCCG CTGCCAGCCTCAGCAGCCCCTAGAACAAACACTGTGACCTCACAGGTCCAGCAGGTCCCAGTTGTACTGC AGCCACACTTCATCAAGGCAGACTCACTGCTGCTGACAGCTGTGAAGACAGATGCAGGAGCCACCGTGAA GACTGCAGGCATCAGCACCCTGGCTCCTGGCACAGCCGTGCAGGCAGGTCCCCTGCAGACCCTGGTGAGT GGAGGGACCATCTTGGCCACAGTACCTTTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTCGCAG CTGGCAGCAAGGCCCTAGGCTCAGCTCAGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAA GCGCTACCGGTCTTCTATCAATGACAAGATTGTGGAGCTCAAAGACCTGGTGGTGGGCACTGAAGCAAAG CTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATCCGCTTCTTGCAGCACAGCAACCAGAAGC TCAAGCAGGAGAACCTGACCCTACGAAGTGCACACAAAAGCAAATCACTGAAGGACCTGGTGTCAGCTTG TGGCAGTGGAGGAGGCACAGATGTGTCTATGGAGGGCATGAAACCCGAAGTGGTGGAGACGCTTACCCCT CCACCCTCAGACGCCGGCTCACCCTCCCAGAGTAGCCCCTTGTCTTTTGGCAGCAGAGCTAGCAGCAGTG GTGGTAGTGACTCTGAGCCCGACAGTCCAGCCTTTGAGGATAGCCAGGTCAAAGCCCAGCGGCTGCCTTC ACACAGCCGAGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGTGTACTGGCCTTTCTGTGTCTGACCTGC AATCCTTTGGCCTCGCTTTTCGGCTGGGGCATTCTCACTCCCTCTGATGCTACGGGTACACACCGTAGTT CTGGGCGCAGCATGCTGGAGGCAGAGAGCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCT AGTCTGGCTGGCCAATGGACTACTAGTGTTGGCCTGCTTGGCTCTTCTCTTTGTCTATGGGGAACCTGTG ACTAGGCCACACTCTGGCCCGGCTGTACACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCC GGGGAGATTTCCCCCAGGCTGCTCAACAGCTGTGGCTGGCCCTGCAAGCGCTGGGCCGGCCCCTGCCCAC CTCAAACCTGGATCTGGCCTGCAGTCTGCTTTGGAACCTCATCCGCCACCTGCTCCAGCGTCTCTGGGTG GGCCGCTGGCTGGCAGGCCAGGCCGGGGGCCTGCTGAGGGACCGTGGGCTGAGGAAGGATGCCCGTGCCA GTGCCCGGGATGCGGCTGTTGTCTACCATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGG ACATCTTGCTGCTTCTAACCTGGCACTAAGTGCCCTCAACCTGGCTGAGTGCGCAGGAGATGCTATCTCC ATGGCAACACTGGCAGAGATCTATGTGGCAGCGGCCCTGAGGGTCAAAACCAGCCTCCCAAGAGCCCTGC ACTTCTTGACACGTTTCTTCCTGAGCAGCGCCCGCCAGGCCTGCCTAGCACAGAGCGGCTCGGTGCCTCT TGCCATGCAGTGGCTCTGCCACCCTGTAGGTCACCGTTTCTTTGTGGACGGGGACTGGGCCGTGCACGGT GCCCCCCCGGAGAGCCTGTACAGCGTGGCTGGGAACCCAGTGGATCCGCTGGCCCAGGTGACCCGGCTAT TCCGTGAACATCTCCTAGAGCGAGCGTTGAACTGTATTGCTCAGCCCAGCCCAGGGGCAGCTGACGGAGA CAGGGAGTTCTCAGATGCCCTTGGATATCTGCAGTTGCTAAATAGCTGTTCTGATGCTGCCGGGGCTCCT GCTTGCAGTTTCTCTGTCAGCTCCAGCATGGCTGCCACCACTGGCCCAGACCCAGTGGCCAAGTGGTGGG CCTCACTGACAGCTGTGGTGATCCACTGGCTGAGGCGGGATGAAGAGGCAGCTGAGCGCTTGTACCCACT GGTAGAGCATATCCCCCAGGTGCTGCAGGACACTGAGAGACCCCTGCCCAGGGCAGCTCTGTACTCCTTC AAGGCTGCCCGGGCTCTGCTGGACCACAGAAAGGTGGAATCTAGCCCAGCCAGCCTGGCCATCTGTGAGA AGGCCAGTGGGTACCTGCGGGACAGCTTAGCCTCTACACCAACTGGCAGTTCCATTGACAAGGCCATGCA GCTGCTCCTGTGTGATCTACTTCTTGTGGCCCGTACCAGTCTGTGGCAGCGGCAGCAGTCACCAGCTTCA GTCCAGGTAGCTCACGGTACCAGCAATGGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATG ACCTGAGCAGCCTGCGGCGGTTGGCACAGAGCTTCCGGCCTGCTATGAGGAGGGTATTCCTACATGAGGC CACAGCTCGGCTGATGGCAGGAGCAAGTCCTGCCCGGACACACCAGCTCCTGGATCGCAGTCTGAGGAGG AGGGCAGGTTCCAGTGGCAAAGGAGGCACTACAGCTGAGCTGGAGCCACGGCCCACATGGCGGGAGCACA CCGAGGCCCTGCTGTTGGCATCCTGCTATCTGCCCCCTGCCTTCCTGTCGGCTCCTGGGCAGCGAATGAG CATGCTGGCCGAGGCGGCACGCACCGTAGAGAAGCTTGGCGATCACCGGCTACTGCTGGACTGCCAGCAG ATGCTCCTGCGCCTGGGCGGCGGAACCACCGTCACTTCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211688 representing NM_011480
Red=Cloning site Green=Tags(s) MDELAFGEAALEQTLAEMCELDTAVLNDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGANSPE SFSSASLASSLEAFLGGPKVTPAPLSPPPSAPAALKMYPSVSPFSPGPGIKEEPVPLTILQPAAPQPSPG TLLPPSFPAPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQPPSSLPLAPAPGVLPTPALHTQVQSLASQQP LPASAAPRTNTVTSQVQQVPVVLQPHFIKADSLLLTAVKTDAGATVKTAGISTLAPGTAVQAGPLQTLVS GGTILATVPLVVDTDKLPIHRLAAGSKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAK LNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTP PPSDAGSPSQSSPLSFGSRASSSGGSDSEPDSPAFEDSQVKAQRLPSHSRGMLDRSRLALCVLAFLCLTC NPLASLFGWGILTPSDATGTHRSSGRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPV TRPHSGPAVHFWRHRKQADLDLARGDFPQAAQQLWLALQALGRPLPTSNLDLACSLLWNLIRHLLQRLWV GRWLAGQAGGLLRDRGLRKDARASARDAAVVYHKLHQLHAMGKYTGGHLAASNLALSALNLAECAGDAIS MATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGDWAVHG APPESLYSVAGNPVDPLAQVTRLFREHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAAGAP ACSFSVSSSMAATTGPDPVAKWWASLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQDTERPLPRAALYSF KAARALLDHRKVESSPASLAICEKASGYLRDSLASTPTGSSIDKAMQLLLCDLLLVARTSLWQRQQSPAS VQVAHGTSNGPQASALELRGFQHDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRR RAGSSGKGGTTAELEPRPTWREHTEALLLASCYLPPAFLSAPGQRMSMLAEAARTVEKLGDHRLLLDCQQ MLLRLGGGTTVTSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011480 |
| ORF Size | 3402 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011480.4 |
| RefSeq Size | 4299 bp |
| RefSeq ORF | 3405 bp |
| Locus ID | 20787 |
| UniProt ID | Q9WTN3 |
| MW | 121 kDa |
| Gene Summary | This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Alternatively spliced transcript variants have been characterized for this gene. [provided by RefSeq, Nov 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205184 | Srebf1 (untagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1), (10ug) |
CNY 7,904.00 |
|
| MR211688L3 | Lenti ORF clone of Srebf1 (Myc-DDK-tagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1) |
CNY 11,780.00 |
|
| MR211688L4 | Lenti ORF clone of Srebf1 (mGFP-tagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1) |
CNY 11,780.00 |
