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Rbl1 (NM_011249) Mouse Tagged ORF Clone
CAT＃: MR211567
- TrueORF®
Rbl1 (Myc-DDK-tagged) - Mouse retinoblastoma-like 1 (p107) (Rbl1), transcript variant 1
ORF Plasmid: tGFP
"NM_011249" in other vectors (3)
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CNY 10,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW547426; p107; PRB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211567 representing NM_011249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGAGGACGAGCCCCACGCCGAGGGGGCGGCGGCGGTCGCCGCGGCCAGGGAGGCGCTGCAGGCCC TGTGCCAGGAACTGAACCTGGACGAGGGAAGCGCGGCCGAAGCCCTGGATGACTTCACGGCCATCCGCGG CAACTACAGCCTAGAGGGAGAAGTTATACACTGGCTGGCATGCTCTTTATATGTTGCTTGCCGCAAGAGC ATCATTCCTACTGTGGGAAAGGGCGTCATGGAAGGAAACTGTGTCTCACTCACCAGAATACTGCGCTCAG CTAAGTTAAGCTTAATACAGTTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAACCTACCACAAGA GTTTCGTGAGCGGATAGAAAGGCTAGAAAGAAATTTTGAAGTATCTACTGTAATTTTTAAAAAATTTGAG CCCATTTTTTTAGATATCTTTCAAAATCCATATGAAGAGCCACCAAAGTTGCCCCGGAGCCGAAAACAGA GGAGGATCCCCTGCAGTGTTAAGGACCTCTTTAACTTCTGCTGGACACTCTTTGTTTACACCAAGGGGAA TTTCCGTATGATTGGTGATGATTTAGTAAACTCCTATCATTTACTTCTGTGCTGCTTGGACCTGATCTTT GCCAATGCTATAATGTGCCCAAATAGACGAGACTTGTTAAATCCATCATTTAAAGGCTTACCATCTGATT TCCATGCTCCGGACTTCAAAGCCGCAGAAGAGCCTCCGTGCATCATTGCTGTACTTTGTGATCTGCATGA CGGACTTCTAGTAGAAGCAAAAGGGATAAAGGAGCATTACTTTAAGCCATATATTTCAAAGCTCTTTGAT AAGAAGATTTTAAAAGGTGAATGCCTCTTGGATCTTTCCAGCTTTACTGATAATAGCAAAGCCGTGAACA AGGAATATGAAGAGTATGTCCTAACTGTTGGGGACTTCGATGAGAGGATCTTTTTGGGAGCAGATGCAGA GGAGGAGATTGGAACACCTCGAAAGTTCACTGCTGACACCCCATTTGGGAAACTGACATCACAGGCCAGT GTGGAATGCAACCTTCAACAACACTTTGAAAAAAAACGGTCATTTGCACCTTCTACCCCACTTACTGGAC GGCGGTATTTACAAGAAAAAGAGGCAGTCACCACGCCTGTAGCTTCAGCCACTCAAAGTGTAAGCCGGTT ACAGAGCATCGTCGCTGGATTAAAAAGCGCACCAAGTGAGCAGCTTCTGAATATTTTTGAATCTTGTATG CGGAATCCTATGGGAAACATTATAAAAATTGTGAAAGGAATAGGAGAGACTTTCTGCCAACACTATACCC AGTCCACAGATAAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGACTAAAACTGGCAGAAATTTT GTATTACAAAATACTGGAGACTATAATGGTCCAGGAAACACGACGGCTTCATGGAATGGACATGTCGGTT CTTTTAGAGCAAGATATATTTCATAAATCCTTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCATATA GCTCACCCCGTACTTTCCCGTGGATTATTGAAGTTCTCGATTTGCAGCCATTTTATTTTTATAAGGTTAT TGAGGTGGTGATCCGCTCAGAGGAGGGCCTTTCCAGAGACATGGTGAAACATCTGAACAGCATTGAAGAA CAGATTTTGGAGAGTTTGGCTTGGACTAATAATTCTGCACTGTGGGAGGCTCTGCATGCTTCTGCAAACA GAGTCCCTTCCTGTGAAGAAGTTATATTCCCTAATAACTTTGAAATAGGTAATGGAGGAAATGTGCAAGG CCATCTCCCCATGATGCCAATGTCTCCAATAATACATCCAAGAGTCAAGGAAGTTCGCACTGACAGTGGG AGCCTTCGACAGGATATGCAACCATTGTCTCCGATTTCTGTCCATGAGCGCTACAGCTCCCCTGCTGCAG GAAGTGCTAAGAGGAGACTGTTTGGAGATGACCCACCAAAGGACACACTGATGGATAAGATTATGGCAGA AGGAACAAAGCTGAAAATTGCTCCTTCGAGTGTCACTGCTGAAAGCTTATCAATTTCCCCTGGGCAAGCT CTTCTGACAATGGCCACAACCACAGTCACAGGGACGACGGGACGGAAAGTTACAGTCCCTTTGCATGGTA TCGCCAATGATGCTGGAGAAATCACACTGGTTCCTATCTCCATGAATCCAACTCAGGAGTCCACAGCTGA GAGCCCTGTGTCACTGACTGCCCAGTCACTAATTGGTACTTCTCCGAAACAGACCCATCTGACTAAAGCA CAAGATGCTCATCTGACCGGAGTAAGCAAACCCAAGAGGACTGGGTCCTTAGCACTGTTCTATAGGAAGG TCTATCATTTGGCAAGTGTGCGCCTACGTGACTTATGTCTAAAACTTGATGTTTCAAATGAATTACGAAG GAAGATCTGGACATGTTTTGAATTCACTTTAGTTCACTGCCCTGATTTAATGAAAGATAGGCATTTGGAT CAGCTCCTTCTGTGTGCATTTTACATCATGGCAAAAGTAACAAAAGAAGAAAGAACTTTTCAAGAAATAA TGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATACAGGAGTGTTCTCTTGAAGAGTATTCC AGGAGGAGTCGTGGTGTACAATGGTGACTGTGAAATGACCGATGGTGACATAGAAGATGCCACAAAGACT CCCAACTGTTCCAGTGAACCAGTGAAAGAGGAAAGAGGTGATCTTATCAAATTTTACAATACAGTATATG TAGGAAGAGTGAAGTCATTTGCATTGAAGTATGATTTATCCAATCAGGACCATATAATGGATGCTCCCCC GCTCTCTCCTTTCCCACACATTAAGCAGCAGCCGGGTTCCCCACGCCGCATCTCCCAGCAGCATTCCCTC TATGTGTCTCCACACAAGAACGGAGCGGGCCTCACCCCCAGGAGCGCACTGCTGTACAAGTTCAACGGCA GCCCTTCTAAGAGTTTGAAAGATATCAACAACATGATAAGGCAAGGCGAGCAGAAAACCAAGAAGCGCGT GATAGCCATCAGTGGAGATGCAGACTCACCTGCCAAACGTCTCTGCCAAGAGAACGATGATGTTTTACTT AAGCGACTACAAGATGTTGTCAGTGAGAGAGCAAATCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211567 representing NM_011249
Red=Cloning site Green=Tags(s) MFEDEPHAEGAAAVAAAREALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVIHWLACSLYVACRKS IIPTVGKGVMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKFE PIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIF ANAIMCPNRRDLLNPSFKGLPSDFHAPDFKAAEEPPCIIAVLCDLHDGLLVEAKGIKEHYFKPYISKLFD KKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTADTPFGKLTSQAS VECNLQQHFEKKRSFAPSTPLTGRRYLQEKEAVTTPVASATQSVSRLQSIVAGLKSAPSEQLLNIFESCM RNPMGNIIKIVKGIGETFCQHYTQSTDKQPGSHIDFAVNRLKLAEILYYKILETIMVQETRRLHGMDMSV LLEQDIFHKSLMACCLEIVLFAYSSPRTFPWIIEVLDLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEE QILESLAWTNNSALWEALHASANRVPSCEEVIFPNNFEIGNGGNVQGHLPMMPMSPIIHPRVKEVRTDSG SLRQDMQPLSPISVHERYSSPAAGSAKRRLFGDDPPKDTLMDKIMAEGTKLKIAPSSVTAESLSISPGQA LLTMATTTVTGTTGRKVTVPLHGIANDAGEITLVPISMNPTQESTAESPVSLTAQSLIGTSPKQTHLTKA QDAHLTGVSKPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLD QLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPGGVVVYNGDCEMTDGDIEDATKT PNCSSEPVKEERGDLIKFYNTVYVGRVKSFALKYDLSNQDHIMDAPPLSPFPHIKQQPGSPRRISQQHSL YVSPHKNGAGLTPRSALLYKFNGSPSKSLKDINNMIRQGEQKTKKRVIAISGDADSPAKRLCQENDDVLL KRLQDVVSERANH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011249 |
| ORF Size | 3189 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011249.2, NP_035379.2 |
| RefSeq Size | 4909 bp |
| RefSeq ORF | 3192 bp |
| Locus ID | 19650 |
| UniProt ID | Q64701 |
| MW | 119.9 kDa |
| Gene Summary | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211567 | Rbl1 (tGFP-tagged) - Mouse retinoblastoma-like 1 (p107) (Rbl1) |
CNY 11,690.00 |
|
| MR211567L3 | Lenti ORF clone of Rbl1 (Myc-DDK-tagged) - Mouse retinoblastoma-like 1 (p107) (Rbl1), transcript variant 1 |
CNY 12,540.00 |
|
| MR211567L4 | Lenti ORF clone of Rbl1 (mGFP-tagged) - Mouse retinoblastoma-like 1 (p107) (Rbl1), transcript variant 1 |
CNY 12,540.00 |
